Biomedical subjects

M E Beker

Publications and source records attributed to M E Beker.

20 records · Page 2Linked to original sources

PubMedDate not supplied

[Protein yield in the rehydration of dehydrated Saccharomyces cerevisiae yeast cells].

The release of proteins was studied during rehydration of Saccharomyces cerevisiae cells dehydrated using two techniques which were characterized by different survival rate. A correlation was established between the loss of proteins and the survival rate of cells after dehydration and rehydration. Proteins with a molecular mass from 6000 to 70,000 daltons (low molecular weight proteins predominating) were found in the rehydration medium by the method of disc-electrophoresis in polyacrylamide gel in the presence of sodium dodecylsulfate. Moreover, the rehydration medium exhibited the activity of certain phosphohydrolases and dehydrogenases; this finding suggests that proteins with a higher molecular weight are present in the medium.

Desiccation↗

PubMedDate not supplied

[Yeast anhydrobiosis: permeability of the cytoplasmic membrane].

When studying the dehydration effect of the eukaryotic cells on the structural and functional characteristics of the cytoplasmic membrane, it was established that serious rearrangements of its molecular organization showing up in a drastic increase in the permeability properties occurred in the yeast when the range of residual humidities of the order of 20-30% was reached. These changes were linked to the beginning of losing some bound water by the cells. It was shown that these rearrangements were reparable, and the normal membrane structure could be restored in the process of slow and gradual rehydration of dehydrated cells in the water vapors.

Adaptation, Physiological↗