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Biomedical subjects

M E Fonseca

Publications and source records attributed to M E Fonseca.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Feto-placentary pathology in human parvovirus B19 infection.

In view of the scarce references concerning the histological data in congenital parvovirus human B19 infection, we intend to provide a description of the pathological features observed in six autopsies. The virus was detected by DNA hybridization (ISH-DBH), PCR and electronmicroscopy (EM) in paraffin-embedded feto-placentary tissues. These cases constitute a subset from 86 Non Immunologic Hydrops Fetalis (NIHF) cases, in which a systemic complex of inflammatory/degenerative lesions of unknown etiology was visualized by optical microscopy. In one case a syphilitic process was detected, typefying a double infection. All fetuses showed a similar pathology--hydrops, hepato-splenomegaly, lung hypoplasia and erythroblastemia, the specific histological feature being the presence of intranuclear inclusions in the erythroid progenitors, in the erythropoietic visceral tissue and in blood marrow. Complex cardiopathy allied to abnormal lung lobulation and polisplenia were observed once; in 2 cases endocardial fibroelastosis was diagnosed. The pulmonary lesions were represented by dysmaturity allied to interstitial mononuclear infiltration. The hepatic consisted of cholestasis, portal fibrosis, canalicular proliferation, hemossiderosis, focal necroses and giant cell transformation. The central nervous system lesions were predominantly anoxic although the autolysis impaired a correct diagnosis.

Erythrocyte Inclusions↗
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Morphological and virological studies in six autopsies of children with adenovirus pneumonia.

Pulmonary lesions compatible with adenovirus infection were detected by gross and microscopic examination of autopsy tissues from children aged from 5 to 34 months. Hepatic lesions indicative of systemic infection were also found in four of the children. The viral etiology was confirmed in three cases by in-situ hybridization, electron-microscopy and immunofluorescence performed in paraffin-embedded tissues, and in one case by cell culture isolation of adenovirus type 2 from nasopharyngeal exudate. Routine testing by methods additional to conventional light microscopy would probably have revealed a larger number of adenovirus infections among the 1,103 autopsy records analyzed in this study.

Adenovirus Infections, Human↗
PubMedDate not supplied

Human papillomavirus detection in genital lesions by in situ hybridization and ultrastructural observations.

Detection of papillomavirus DNA in situ hybridization technique was performed in 29 symptomatic patients (6 males and 23 females) during the period of 1989-1991 at the Clinic for Sexually Transmitted Diseases, Universidade Federal Fluminense, State of Rio de Janeiro. All the male patients had condyloma acuminata. Only HPV 6/11 were found in these lesions. Clinical features in the female patients included vulvar condyloma acuminata, bowenoid papulosis, flat cervical condyloma, cervical condyloma acuminatum and cervical intraepithelial neoplasia grade II (CIN II). We also found cases of condyloma acuminata associated to vulvar intraepithelial neoplasia grade III (VIN III), as well as to vaginal invasive carcinoma. HPV 6/11 and 16/18 were found in vulvar condyloma acuminata. Mixed infection by 6/11-16/18 HPV were also seen in these lesions as well as in the patient who had cervical condyloma acuminatum. HPV 16/18 were found in the condyloma acuminatum plus VIN III and in the CIN II lesions. We have found HPV 31/33/51 in the specimen of condyloma acuminatum plus invasive carcinoma. In order to investigate the ultrastructural aspects of HPV infection in genital tissue, the biopsies of three female patients were observed under electron microscope. Mature virus particles were found in the cells of a condyloma acuminatum as well as in the condyloma acuminatum plus invasive carcinoma case. In another sample, chromosome breakages were found in the nuclei of the infected cells although no viral particles were observed.

Adult↗
PubMedDate not supplied

Prevalence of human papillomavirus DNA in female cervical lesions from Rio de Janeiro, Brazil.

A hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (HPV) types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. The data were compared with histologic diagnosis. Eighty-eight (55%) biopsies contained HPV DNA sequences. In low grade cervical intraepithelial neoplasias (CIN I), HPV infection was detected in 78.7% of the cases, the benign HPV 6 was the most prevalent type. HPV DNA was detected in 58% of CIN II and CIN III cases and in 41.8% of squamous cell carcinomas (SCC). Histologically normal women presented 20% of HPV infection. Oncogenic HPV was found in 10% of these cases, what may indicate a higher risk of developing CINs and cancer. Twenty-five percent of the infected tissues contained mixed infections. HPV 16 was the most common type infecting the cervix and its prevalence raised significantly with the severity of the lesions, pointing its role in cancer pathogenesis. White women presented twice the cervical lesions of mulatto and African origin women, although HPV infection rates were nearly the same for the three groups (approximately 50%). Our results showed that HPV typing by in situ hybridization is a useful tool for distinguishing between low and high risk cervical lesions. Further studies are required to elucidate risk factors associated with HPV infection and progression to malignancy in Brazilian population.

Brazil↗
PubMedDate not supplied

Studies on the replication of Mayaro virus grown in interferon treated cells.

Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.

Aedes↗
PubMedDate not supplied

Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil.

Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1982, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease.

Brazil↗
PubMedDate not supplied

[Decrease in circulating androgens in men during senescence].

It has been reported that circulating androgens exhibit a decrement with aging in men; however, there are some controversial results. We have carried out a descriptive study in a group of men aged 60-70 years. In order to determine levels of androgens In serum, the results were compared with those obtained in another group in normal men 20-44 years of age. Measurements included circulating levels of testosterone(T), dehydroepiandrosterone sulfate (DHEA-S), LH, FSH, estradiol (E), ACTH, prolactin (PRL), IGF-1, and SHBG. The group of older men group showed a significative decrement of T and DHEA-S, as well as E. An increment of FSH and LH without changes of ACTH, cortisol and SHBG was demonstrated. Collaterally, PRL and IGF-1 decreased in aging men. The present data demonstrated a significant decrement of circulating androgens, both T and DHEA-S coinciding with elevation of gonadotropins. It is concluded that aging is accompanied by changes of a decrement of androgens, of gonads, and adrenal origin. Further studies will investigate the effect of testosterone replacement in aging men.

Adult↗
PubMedDate not supplied

[Helicobacter pylori infection in a group of HIV/AIDS patients].

The prevalence of H. pylori infection in AIDS patients has been controversial. A. controlled prospective and descriptive study was conducted in a group of 64 HIV-infested patients. H. pylori detection was carried out by endoscopic study during which two biopsy samples of gastric antrum and body were taken for the histologic study and other 2 samples of gastric antrum and body for urease testing. H.pylori infection prevalence was 56.3%. The comparison group was composed by 110 serologically HIV-negative patients, with an infection prevalence rate of 77.2%. In the HIV-AIDS group, the frequency of gastric ulcer was 1,5% and no duodenal ulcer was found whereas the comparison group showed a frequency of gastric and duodenal ulcers of 1.8 and 15.5% respectively. HIV+/AIDS patients in category I exhibited an H. pylori infection prevalence of 68.7%, category II, 39.2% and category III, 21,0%. A reversed association was observed between the level of immune deterioration and the prevalence of H. pylori infection.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Molecular heterogeneity of prolactin and gonadotropins in some forms of sterility].

In this paper are presented some chromatographic studies for partition of different molecular forms of prolactin (PRL), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in infertile women. Serum from three patients with prolactinoma and six with premature ovarian failure were fractionated in Sephadex G-100, and eighty fractions were collected, where PRL or FSH/LH were measured by radioimmunoassay, according with each case. Patients with prolactinoma showed two different chromatographic patterns for PRL. Two women with secondary amenorrhea and galactorrhea showed predominance of "little" PRL. In contrast, the patients with primary amenorrhea and galactorrhea showed only "big" PRL and "big-big" PRL. Among the patients with premature ovarian failure, two had chromosomal mosaicism, two had refractory ovarian syndrome and the other two had idiopathic premature ovarian failure. In these patients were found the presence of several molecular forms of FSH and LH, with different chromatographic profiles to those observed in normal young women and postmenopausal women. These results are indicating the presence of several molecular forms of pituitary hormones in patients with prolactinoma and premature ovarian failure.

Adult↗