[Transformation of mitochondria in sebaceous gland cells of the hare Lepus timidus L].
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Biomedical subjects
Publications and source records attributed to M E Kriukova.
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Histology and ultrastructure of sebaceous glands of the integumental inguinal gland of the hare were studied during the periods of low and high reproductive activity (in winter and in spring breeding season). It was established that the glands were functioning continually (cells in acini of all the animals used contained numerous secretory granules). The expansion of agranular endoplasmic reticulum and Golgi complex in secretory cells of sexually active animals, especially of males, shows an increased intensity of gland secretory process during the breeding season.
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Dominant autosomal mutation l(2)M66 DCS induced in Drosophila melanogaster by ethyl-methane-sulfonate was studied. Electron-microscopic studies of asynchronous (fibrillar) and synchronous (tubular) muscles in 24-hour old mutants showed pathological changes in their fine structure. All systems were affected: the fragmentation of the Z-lines, disappearance of protofibrils, degenerative changes of mitochondria, sarcoplasmic reticulum, and the T-system, the appearance of membrane aggregates and lysosomes, the presence of a large amount of glycogen were detected. These changes in the ultrastructure of the flight muscles in mutants are similar to those observed in the process of physiological degeneration of insect muscles.
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Ultrastructure of indirect flight muscles was analyzed in Drosophila pupae heterozygous for the mutation l(2)MhcM66, which is an allele of the locus Mhc (Myosin heavy chain). It is demonstrated that the initial stages of muscle fiber differentiation in mutants proceed normally. First visible changes (elimination of individual thick protofibrils) are observed when myofibrils attain a certain size. Disintegration of the contractile apparatus progresses with growth of myofibrils. Mechanisms responsible for onset and development of these destructive processes during ontogeny are discussed.
The ultrastructure of indirect flight muscle was studied in flies (imago) heterozygous for a new allele of the gene of heavy myosin chain. Analysis of the observed defects of myofibrils suggests that in the mutants completion of differentiation of the flight muscle is disturbed.