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Biomedical subjects

M E Lane

Publications and source records attributed to M E Lane.

At least 37 records · Page 2Linked to original sources
PubMed1983

Undergraduate curricula in PM&R: implications for resident recruitment and general image of the specialty.

Comprehensive data on undergraduate curricula in Physical Medicine and Rehabilitation were obtained in a 1980 questionnaire survey of approved residency programs. Survey parameters included curriculum content (type and duration per academic year), teaching format and educational methodologies, academic exposure and student enrollment, faculty characteristics, and curriculum impact on resident recruitment. Of 53 responding programs 44 reported undergraduate education activities in PM&R. Curriculum content was predominantly clinical in all programs. The teaching format used by 70% of respondents consisted of a combination of lectures, clinical case presentations, conferences, demonstrations and clinical clerkships. Seventy-two percent of this sample were either compulsory or contained a compulsory component. An academic exposure to PM&R of two or more years and a curriculum duration of more than four weeks per academic year was reported by 81% and 75% of respondents respectively. Compulsory programs were offered most frequently in the first two years, while electives were offered mostly in the last two years of study. A low enrollment in electives (less than 50% of class) is reported, regardless of year of study. A teaching faculty of more than five was reported by 63% of programs. A positive curriculum impact on resident recruitment was reported by 72% of the respondents. Positive and negative implications of these findings for the PM&R specialty image and for a career choice in PM&R are discussed.

Attitude↗
PubMedDate not supplied

Glutamine synthetase: a key enzyme for intestinal epithelial differentiation?

BACKGROUND: We have previously shown that glutamine synthetase protein and mRNA are concentrated in the crypt region of the rat small intestine and that the activity of this enzyme is highest around the time of weaning. This anatomical location and time of peak activity are sites and periods of active enterocyte differentiation. This led to our current hypothesis that glutamine synthetase is important in the differentiation of enterocytes. METHODS: To test our hypothesis, we treated Caco-2 cells with physiologic (0.6 mM) glutamine concentrations in cell culture medium. The experimental group was treated with methionine sulfoximine, an irreversible glutamine synthetase inhibitor, and the control group with phosphate buffered saline. Three standard and well-defined markers of intestinal differentiation-sucrase-isomaltase activity, microvillus formation, and electrical impedance in transwell plates-were compared between the two groups. RESULTS: The methionine-sulfoximine-inhibited group was found to have lower sucrase-isomaltase activity, a lower density of microvilli, and lower electrical impedance values over time compared with the control group. CONCLUSION: The experimental group was found to be less differentiated by all three markers of differentiation. Therefore, glutamine synthetase is important for Caco-2 cell differentiation.

Caco-2 Cells↗
PubMedDate not supplied

Successful use of the diaphragm and jelly by a young population: report of a clinical study.

In the largest contemporary study of diaphragm use in the United States, the authors examine the experience of 2,168 women who selected this method of contraception at the Margaret Sanger Research Bureau over a recent two-year period. Eight in 10 of the study group were younger than 30 years and three in 10 were aged 21-24. Seven in 10 had never been married and the same proportion had never been pregnant. Accidental pregnancies in the first 12 months of use ranged from a low of 1.9 per 100 users younger than 18 years old to a high of 3.0 among 30-34-year-olds; and more than eight in 10 were still using the diaphragm at the year's end. These rates compare favorably with those reported for the pill and IUD in other clinical studies.

Adolescent↗