GROWTH CHARACTERISTICS OF THE BLUE-GREEN ALGAL VIRUS LPP-1.
Safferman, Robert S. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), and Mary-Ellen Morris. Growth characteristics of the blue-green algal virus LPP- 1. J. Bacteriol. 88:771-775. 1964.-The blue-green algal (BGA) virus, strain LPP-1, formed two distinct plaque variants. During subsequent propagation, each of the isolated variants eventually reverted to a mixture of both plaque types. The large-plaque-former, multiplying at a somewhat faster rate, showed maximal virus production within 60 hr after infection; maximal titer of the small-plaque mutant was reached after a 76-hr incubation period. No differences were observed in either their host spectra or their pH and thermal stability. Evidence was presented which demonstrated that BGA virus samples could be assayed with reasonable accuracy from plaque counts. Variations resulting from this plating technique were within the range of experimental error that has been generally reported for such a system.