PubMed Health⌕ Search

Biomedical subjects

M E Milham

Publications and source records attributed to M E Milham.

6 recordsLinked to original sources

Optical properties of Erwinia herbicola bacteria at 0.190-2.50 microm.

We measure the complex index of refraction of Erwina herbicola (also known as Enterobacter agglomerans or Pantoea agglomerans) bacteria (ATTC 33243) over the spectral region from 0.190 to 2.50 microm (4000-52,632 cm(-1)). Transmission measurements are made on solid films of E. herbicola and on suspensions of the bacteria in water. These measurements, combined with spectral reflectance and Kramers-Krönig analysis, allow the determination of the real and imaginary parts over the entire wavelength interval. Accurate and consistent results are obtained for this complex and difficult to measure material. This is part of a continuing series of measurements of the optical constants of representative biological materials that are applicable to the development of methods for detection of airborne biological contaminants, where the material under study is used as a surrogate for a pathogenic agent.

Bacteria↗

Optical properties of ovalbumin in 0.130-2.50 microm spectral region.

In our continuing series of measurements of the complex index of refraction for representative samples of biological materials, we measured ovalbumin (egg albumin) over the spectral region from 0.130 (76,923 cm(-1)) to 2.50 microm (4000 cm(-1)). Films of ovalbumin suitable for optical analyses were prepared and measured in addition to solutions of ovalbumin in water. We show several examples of how the methods used in this study produced accurate results for this complex and difficult to measure material. The present work is applicable to quantitative optical studies involving ovalbumin and other serpin proteins, as well as the study of proteinaceous toxins.

Animals↗

[Not Available].

Explore the source record for details and available documents.

Bibliographies as Topic↗