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Biomedical subjects

M E Peetermans

Publications and source records attributed to M E Peetermans.

At least 55 records · Page 3Linked to original sources
PubMed1986

Flow cytometric analysis of hairy cell leukemia using right-angle light scatter.

We report on an easy and reliable method for the enumeration of the typical cells present in the blood, bone marrow, and spleen of patients with hairy cell leukemia. Samples of five patients were analyzed. In three patients, the hairy cells were very accurately followed during treatment with alpha-interferon, using right-angle light scatter to differentiate them from other leukocytes present in total blood. Excellent correlation was obtained with the microscopic hairy cell enumeration. Additional verification by cell sorting and immunofluorescence studies confirmed these findings.

Cell Count↗
PubMed1986

Treatment of hairy-cell leukemia with recombinant alpha 2-interferon.

Eleven patients with hairy-cell leukemia (eight with progressive and three with non-progressive disease) were treated with low dose recombinant human alpha 2-interferon. After a 3-month treatment period, nine patients showed an improvement and one patient a partial remission. By then, transfusions were not required any more and serious infections were no longer encountered. Four patients were further treated: three for a total period of 9 months and one for 6 months; all of them reached a partial or complete remission. The treatment was equally effective in patients with both progressive and non-progressive disease. Previous absence of response to splenectomy did not preclude a positive effect of IFN therapy. In two patients, IFN dose reduction was necessary due to unremitting flu-like symptoms.

Humans↗
PubMed1986

Flow cytometric analysis of erythrocytic D antigen density profile.

Red blood cells (RBC) were studied with flow cytometry (FCM) after staining with a directly fluoresceinated anti-D antiserum. The immunofluorescence distribution profiles were slightly asymmetrical, due to a significant, yet small skewness. This skewness was not associated specifically with D antigen positivity, making a homogeneous, normal D antigen distribution on the RBC very likely. With the exception of Du, D-positive RBC could always be distinguished from D-negative ones. FCM was successful in bringing the following phenomena to light: difference in mean fluorescence intensity between the different rhesus phenotypes, the D gene dosage effect, the depressing effect of C antigen on D antigenicity, the nonspecific attachment of IgG on (D-negative) RBC and rhesus mosaicism. The absolute number of D antigen sites could be calculated, using an absolute fluorescence standard.

Erythrocytes↗
PubMed1986

Purpura fulminans in pneumococcal sepsis.

Two cases of pneumococcal sepsis in splenectomized patients were complicated by purpura fulminans. In addition, acute renal failure developed in both patients, and myolysis in one. Immunological findings in the patient with myolysis suggest a possible role of pneumococcal antigen-containing circulating immune complexes in the pathogenesis of these complications.

Acute Kidney Injury↗
PubMed1985

A myelodysplastic syndrome preceding acute lymphoblastic leukaemia.

The bone marrow of a patient with pancytopenia showed dyserythropoiesis, dysmegakaryocytopoiesis and 13% blasts. The patient was hypertransfused and the pancytopenia resolved completely for 1 month, while the blastic infiltration in the bone marrow remained. Three months later a frank acute lymphoblastic leukaemia developed.

Bone Marrow↗
PubMed1983

Usefulness of apheresis to extract microfilarias in management of loiasis.

Two patients with loiasis in whom treatment with diethylcarbamazine had to be interrupted because of serious side effects underwent apheresis to extract microfilarias from the blood. After three apheresis sessions the number of microfilarias in the blood had dropped from 8980 to 3412/ml in one patient and from 2950 to 1840/ml in the other. Between 40 and 45 X 10(6) microfilarias were removed from each of the patients. After reduction of the parasitaemia the two patients were given diethylcarbamazine; no appreciable side effects occurred, and the patients were cured.

Adult↗
PubMed1982

HLA-DR and monoclonal gammopathy.

The frequencies of the HLA-DR antigens were compared in 3 groups: a group of 33 patients with multiple myeloma (MM) or Morbus Waldenström (MW): a group of 34 persons with benign monoclonal gammopathy (BMG); and a group of 118 healthy controls. No statistically significant associations were observed.

Adult↗
PubMed1979

Practical immunoevaluation in anesthesia.

Postoperative infection and cancer metastasis suppose weakened immunological resistance. Immunoevaluation is difficult for technical reasons and for individual variability. Proposed tests are hematology, cytochemistry, immunoglobulin and complement assays, membrane and cytoplasmic fluorescence, rosettes, lymphocyte transformation tests, skin tests, tests for granulocyte and monocyte function. Material and methods should be well standardized. Frozen serum and lymphocytes are peremptory for cellular and humoral evaluation during the different phases of anesthesia.

Anesthesia↗
PubMed1979

Immunological investigation in adenine phosphoribosyl transferase (APRT) deficiency.

In a family with partial or total adenine phosphoribosyl transferase (APRT) deficiency, T and B cell function was evaluated by counting rosette-forming cells, by investigating lymphocyte transformation to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), by assaying serum immunoglobulins and determining lymphocyte membrane immunoglobulins by immunofluorescence. Delayed cutaneous hypersensitivity was also assayed. The absence of gross immunological deficiency in the homozygotes, as well as in the heterozygotes, suggests that the APRT-mediated pathway of purine metabolism is relatively unimportant for lymphocyte function.

Adenine Phosphoribosyltransferase↗