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Biomedical subjects

M E Pochtar'

Publications and source records attributed to M E Pochtar'.

8 recordsLinked to original sources
PubMed2002

[Cytokine system in patients with chronic hepatitis C during treatment with interferon-alfa].

AIM: To study changes in serum levels of interleukine-1 beta (IL-1b), IL-6, TNF-alpha (TNFa), HM-HMF and TFR-1 beta (TFR-1b), expression of surface antigens CD14 and CD95 on blood monocytes from patients with chronic hepatitis C (CHC) treated with interferon-alpha (INFa). MATERIAL AND METHODS: Examinations covered 25 CHC patients and 25 healthy controls. Concentrations of proinflammatory cytokines and growth factors in blood serum were measured with ELISA (kits by "R&D systems", USA). CD14 and CD95 antigen expression on monocytes of venous blood were studied using flow cytoflowmeter (Partes, USA) before and after a 12-week course of INFa. RESULTS: Before INFa treatment CHC patients had significantly elevated serum concentrations of TNFa, HM-KSF and TFR-1b. Coexpression of antigens CD14+ and CD95+ was found on 61% of blood monocytes. Three-month INFa treatment lowered levels of TNFa, GM-KSF and CD95+ expression on monocytes as well as TFR-1b concentration in the serum which correlated with a positive trend in the standard clinicolaboratory and virusological indices in the examinees. CONCLUSION: Changes in serum indices of proinflammatory cytokines and growth factors, in expression of CD95 on blood monocytes from CHC patients treated with INFa show an important role of cytokines system activation and mechanisms of programmed cell death in pathogenesis of chronic HCV infection.

Adolescent↗
PubMed2001

[Cytokine production in patients with chronic viral hepatitis C during treatment with interferon-alpha].

Serum content of proinflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) and growth factors (GM-CSF, TGF-1 beta) and expression of CD14 and CD95 antigens on peripheral blood monocytes before and after 12-day therapy with alpha-interferon were studied in 25 patients with chronic viral hepatitis C (VHC). The concentrations of TNF alpha, GM-CSF, and TGF-1 beta were significantly increased (p < 0.05) and coexpression of CD14+ and CD95+ antigens on monocytes was increased by 61% in VHC patients in comparison with the control. After 3 months of therapy with alpha-interferon, the content of TNF alpha, GM-CSF, and TGF-1 beta essentially decreased and that of IL-6 increased; this was paralleled by improvement of clinical and laboratory parameters and decrease of coexpression of CD14+ and CD95+ antigens on blood monocytes. Modulation of the functions of immunocompetent cells and changed production of cytokines are apparently one of the mechanisms of inhibitory effect of alpha-interferon on HCV infection. Study of proinflammatory cytokines and growth factors in the serum and expression of CD14 and CD 95 antigens on monocytes can serve as additional tests for evaluating the efficiency of interferon therapy in patients with VHC.

Adolescent↗
PubMed1996

[The prognostic criteria in the primary myelodysplastic syndrome].

Basing on computer processing of 126 primary clinical and laboratory parameters obtained from 92 patients with myelodysplasia and using multifactorial regression analysis, the authors have developed prognostic models of life span and probability of transformation into acute leukemia. The model of life span enabled recognition of 3 prognostic groups of myelodysplasia patients: of high (median 10 months), moderate (median 22 months) and low (median 35 months) risk. This makes it possible to prognosticate the disease and assume optimal therapeutic policy.

Adult↗
PubMedDate not supplied

[A comparative evaluation of the differential count of peripheral blood leukocytes using the Cell-Dyn 3500 and NE-7000 hematology analyzers].

Two blood analyzers, Cell-Dyn 3500 (Abbott) and NE-7000 (Sysmex) are compared as regards their use in clinics of a general profile. Both devices can differentiate the leukocytic formula by 5 parameters (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) and possess a system of alarm signals indicating changes in the leukocytic formula. A total of 165 peripheral blood samples stabilized with K3-EDTA were examined. Visual assessment of stained preparations, 200 cells per smear, was the reference method. Both devices yielded similar results of leukocyte counting, were in good correlation with the visual method in assessment of neutrophils, lymphocytes, and eosinophils. Both devices proved to be inaccurate in counting monocytes, which was due to a great variety of morphologic forms of these cells. In addition, the number of false-positive and false-negative results was assessed. No abnormalities were detected in the 20 samples which were assessed as "negative" by the analyzers. Study of the peripheral blood by automated blood analyzers Cell-Dyn 3500 and NE-7000 helps detect a pathological leukocytic formula in 100% cases, although the type of shifts is not always precisely assessed by these devices. Therefore, morphological control is needed in case of alarm signals.

Conductometry↗