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M F Barile

Publications and source records attributed to M F Barile.

At least 91 records · Page 5Linked to original sources
PubMed1972

Isolation and characterization of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis.

Fourteen conjunctival strains of mycoplasma were isolated from infected sheep and goats in two separate naturally occurring outbreaks of keratoconjunctivitis (pink-eye). The biological and serological properties and cell protein patterns of these strains are presented in this report. These strains were related to each other and were unrelated to 40 recognized Mycoplasma and Acholeplasma species and serotypes. We are proposing that this group of mycoplasmas be named M. conjunctivae. The role of M. conjunctivae in the etiology of keratoconjunctivitis in sheep and goats remains to be determined.

Animals↗
PubMed1971

United States standard diphtheria toxin for the Schick text and the erythema potency assay for the Schick text dose.

A diphtheria toxin shown to have high toxicity and avidity and to combine with antitoxin in multiple proportions was selected for the U.S. standard diphtheria toxin for the Schick test and freeze-dried. Assayed values per vial were 1.09 L(f), 1.09 L(r), 1,090 Schick test doses (STD), 33 LD(80), 38 LD(50), and 43,000 minimum skin reactive doses. One STD (L(r)/1000) is slightly more toxic than one unit of the international standard (L(f)/1,000). Experiments showed that the potency assay of Schick test toxin by guinea pig erythema toxicity, determined relative to the toxicity of the standard, was highly reproducible and significantly more reproducible than the lethal (minimum lethal dose) test and that the STD, defined as one L(r)/1000, was equivalent to approximately 1/50 minimum lethal dose. The erythema potency assay was prescribed in the U.S. standards for Schick test toxin effective in 1969.

Animals↗
PubMed1971

Proposal for classifying human strain navel and related simian mycoplasmas as Mycoplasma primatum sp. n.

Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the established Mycoplasma and Acholeplasma species of the class Mollicutes. It is proposed that strains with the properties described be named Mycoplasma primatum.

Animals↗
PubMed1970

Immunization against neonatal tetanus in New Guinea.

THIS PAPER DESCRIBES: (1) a continuation of the study reported in 1965 of tetanus antitoxin titres among women after primary immunization with plain, AlPO(4)-adsorbed or oil adjuvant toxoids; (2) the effect of age and of abscess formation due to oil adjuvants on antitoxin response; and (3) a comparative study of titres in some women of the study groups who received either a plain or an AlPO(4) toxoid booster injection in pregnancy.The results support our previous recommendation to use aluminium adjuvant toxoid in the prevention of neonatal tetanus. Mean maternal protective antitoxin levels were maintained for 40 months but not for 54 months after 2 primary injections and booster response was higher in those immunized with this toxoid than in those immunized with plain toxoid. Also, the use of AlPO(4) toxoid as booster stimulated a higher antitoxin response than did the use of plain toxoid. Mean protective levels induced by oil adjuvant toxoids (1 injection) persisted for the same time as for the adsorbed toxoid (2 injections). Age did not significantly affect the level of antitoxin response, whereas abscess formation was associated with a higher level.Pregnancy appeared to favour the incidence of antitoxin responders to adsorbed toxoids. Failure to respond to primary immunization with 2 injections of adsorbed toxoid occurred in 7% of women while 100% responded to the oil adjuvant toxoids. Induction of abscess formation precludes use of the latter toxoids, however. Additional studies are needed on the influence of larger antigen doses on the primary response in different ethnic and ecological situations and on the influence of smaller antigen doses on the booster response where repeated booster injections are indicated.

Adjuvants, Immunologic↗
PubMed1970

Immunization against neonatal tetanus in New Guinea. 3. The toxin-neutralization test and the response of guinea-pigs to the toxoids as used in the immunization schedules in New Guinea.

The mouse toxin-neutralization test procedure described has been used for antitoxin titration of sera of New Guinea women following primary immunization with plain and adjuvant tetanus toxoids and following booster immunization, and for titration of guinea-pig sera.The responses of guinea-pigs immunized with the same toxoids and immunization schedule to simulate the human field trial studies were observed for 6 months. The relative antitoxin responses of guinea-pigs to the different toxoids were similar to those of the women.

Animals↗
PubMed1970

Immunization against neonatal tetanus in New Guinea. 4. Comparison of tetanus antitoxin titres obtained by haemagglutination and toxin neutralization in mice.

Haemagglutination (HA) has been used frequently for the titration of tetanus antitoxin but published results have varied in relation to the "unitage" determined by the mouse toxin-neutralization test (TN). This report gives the results of the titration of a group of sera by haemagglutination and compares them with the results obtained by toxin neutralization. Although there was marked variation between HA and TN titres of individual sera, results indicated that haemagglutination is a useful procedure for the over-all evaluation of the antitoxin responses to tetanus toxoids in field studies.

Animals↗
PubMed1970

Immunization against neonatal tetanus in New Guinea. 5. Laboratory assayed potency of tetanus toxoids and relationship to human antitoxin response.

Previous papers in this series have shown that plain toxoids induced early primary antitoxin levels in women in New Guinea that were not significantly different from those induced by adsorbed toxoids but that at the end of 1 year the antitoxin levels differed significantly. Protective levels (not less than 0.01 unit/ml) induced by adsorbed toxoids persisted for more than 3 years. Results of laboratory assays of the toxoids reported in this paper show that per total human immunizing dose, the plain toxoids had 72 or less international units (IU) whereas the adsorbed toxoids had approximately 200 IU. The international "unitage" of these toxoids reflected the persistence of the human protective antitoxin level but not the early primary response. The assay results were in agreement with findings of other workers that the mouse as well as the guinea-pig may be satisfactory for potency assay of adsorbed toxoids. The need for determination of the international unitage of tetanus toxoids used in human studies and the confirmation of relationship of this value to persistence of antitoxin levels is emphasized.

Animals↗