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M F Braña

Publications and source records attributed to M F Braña.

26 records · Page 2Linked to original sources
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The pharmacokinetics, tissue distribution, and biotransformation of a new class of antitumor agents: mitonafide and pinafide.

The pharmacokinetics, biotransformation, protein binding and tissue distribution of mitonafide and pinafide were studied after single i.v. and oral administration of each drug (20 mg/Kg) in female rats. In pregnant rats a study of cross placental-barrier after i.v. administration of the two drugs was also performed. The drugs were absorbed fairly rapidly with a mean peak plasma level of 7.63 +/- 0.70 micrograms eq/ml for the 3H-mitonafide and with 6.16 +/- 0.77 micrograms eq/ml for the 3H-pinafide between 30 minutes and 1 hour after oral dosing. For both drugs, the pharmacokinetics can be described by a two-compartment open model. The mean elimination half-lives were 17.8 h and 47.5 h for 3H-mitonafide and 3H-pinafide, respectively. Two metabolites for each compound as well as unchanged drugs were identified in urine by TLC and GC/MS by comparison of their chromatographic properties with a number of reference compounds. Approximately 30% of the radioactive drug was excreted over a 48 h period for 3H-mitonafide and 24% for 3H-pinafide in urine, after i.v. administration. The cross placental-barrier studies showed that both 3H-mitonafide and 3H-pinafide were present in the 14-day fetuses.

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PubMedDate not supplied

Fluorescence of chromatin DNA induced by antitumoral naphthalimides.

Treatment of chicken blood smears and semithin sections from Epon-embedded mouse tissues with aqueous solutions of the 3-aminonaphthalimides FA-142, FA-2043, and FA-2143 induced a strong green-yellow fluorescence of chromatin under violet or violet-blue excitation. Chromatin emission was abolished by previous DNase or hot TCA treatment. The use of 3-methoxy (FA-655) and 3-nitro derivatives (M-4212 and M-12210) resulted in very weak fluorescence of chromatin. Absorption maxima at 346 and 408 nm and an emission peak at 570 nm were observed for the free compound FA-142. Fluorescence properties open new and interesting applications for some of these antitumoral and DNA-intercalating naphthalimides.

Animals↗