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M F Cohen

Publications and source records attributed to M F Cohen.

5 recordsLinked to original sources
PubMed1992

Dynamic frequency change among stimulus components: effects of coherence on detectability.

Experiments were performed to determine the effect of coherent dynamic frequency change among stimulus components on detection of one of those components. Detectability of a frequency glide signal, centered at 660 Hz, was measured in the presence of two additional frequency glides centered at 220 and 440 Hz. Results show that the signal is most difficult to detect when it is changing coherently with the other stimulus components, and that detectability gradually improves as the frequency change among stimulus components becomes less coherent. A control experiment indicated that detectability of the signal is influenced somewhat by the average spectral distance between the signal and the other stimulus components. In order to separate the effects of dynamic frequency change from those of altering harmonic relationships, the experiment was repeated with stimulus components not harmonically related. Results are similar in pattern, though with somewhat smaller shifts. A final experiment was performed to determine if the observed shifts in detectability might be due to changes in the maximum spectral distance between signal and masker when their frequencies are changing incoherently. Results of this experiment indicate that detectability improves when frequency change of signal and masker is not coherent, even when maximum spectral distance does not change. These data indicate the likelihood that the auditory system is able to use coherent dynamic frequency change among stimulus components.

Adult↗
PubMed1991

Comodulation masking release and the masking-level difference.

An experiment was performed to determine if the mechanism that mediates comodulation masking release (CMR) is associated with that used to improve detection by the masking-level difference (MLD). The experiment consisted of first improving detectability of a masked diotic tone burst by adding a synchronous noise band at another frequency region (CMR), and then measuring an MLD in the usual manner, by inverting the tone-burst signal to one ear. Results indicate that a substantial MLD can be measured for a signal whose detectability has already been improved by CMR. However, that MLD (9 dB) is smaller than that measured in random noise (14 dB). Put another way, a small CMR (4 dB) can be produced even when the detectability of a stimulus has already been improved due to the MLD. These data are in general agreement with those of Hall et al. [J. Acoust. Soc. Am. 83, 1839-1845 (1988)] and Schooneveldt and Moore [J. Acoust. Soc. Am. 85, 262-272 (1989)].

Adolescent↗
PubMed1990

The cytoskeletal system of mammalian primitive erythrocytes: studies in developing marsupials.

Seeking to resolve conflicting literature on cytoskeletal structure in mammalian "primitive" generation erythrocytes, we have utilized the circulating blood of developing marsupials. In young of the Tammar Wallaby (Macropus eugenii) and the Gray Short-tailed Opossum (Monodelphis domestica), relatively large, nucleated primitive erythrocytes constituted nearly 100% of the circulating population at birth (= day 0) and in fetuses (Tammar) several days before birth. These cells were discoidal or elliptical, and flattened except for a nuclear bulge. Their cytoskeletal system, consisting of a marginal band of microtubules enclosed within a cell surface-associated network (membrane skeleton), closely resembled that of non-mammalian vertebrate erythrocytes. By day 2 or 3, much smaller anucleate erythrocytes of "definitive" morphology, lacking marginal bands, appeared in abundance. These accounted for greater than 90% of the circulating population of both species by day 6-8. Non-nucleated erythrocytes of a different type, constituting 1-6% of the cells in most blood samples up to day 7, were identified as anucleate primitives on the basis of size, shape, and presence of a marginal band. Thus, loss of erythrocyte nuclei in mammals appears to begin earlier than generally recognized, i.e., in the primitive generation. Counts of these anucleate primitives in young of various ages implicated nucleated primitives as their probable source. Pointed erythrocytes, occasionally found in younger neonates of both species, occurred in greatest number in fetuses (Tammar) prior to birth. This is in accord with previous work on non-mammalian vertebrates suggesting that such cells are morphogenetic intermediates. The results confirm the long-suspected similarity between mammalian primitive erythrocytes and the nucleated erythrocytes of all non-mammalian vertebrates.

Animals↗
PubMedDate not supplied

Direct regulation of Na(+)-dependent myo-inositol transport by sugars in retinal pigment epithelium: role of phorbol ester and staurosporin.

An Na(+)-dependent active process for myo-inositol (MI) uptake, sharing a common carrier system with glucose and sensitive to phlorizin, was previously established in primary cultures of bovine retinal pigment epithelial (RPE) cells (26, 32). The present report further examines the nature of glucose-induced inhibition of MI transport in primary cultures of RPE cells. RPE cells were grown in supplemented Dulbecco's modification of Eagle's medium (DMEM) containing 5 mM D-glucose (basic growth media) or 40 mM D-glucose or its nonmetabolizable analogue, alpha-methyl-D-glucoside (alpha MG); 1-5 mM nonradioactive MI, pyruvate, or lactate; or 0.2-20 microM phorbol 12-myristate 13-acetate (TPA) or straurosporin (modified growth media), for up to 4 weeks. The capacity of RPE cells to accumulate 3H-MI (ratios of intracellular transported radioactive MI, [MI]i, to external free MI concentration, [MI]i/[MI]o) decreased by up to 41% or 34% when cells were grown for 10 days or longer with 40 mM D-glucose or 40 mM alpha MG, respectively, compared to cells grown in basic growth media. The rate of uptake of 3H-MI also was reduced to 63 +/- 15% or 48 +/- 8% of the control values when cells were fed 1 or 5 mM nonradioactive MI, respectively. In addition, cellular capacity to bind to [3H]phlorizin was reduced to 52 +/- 7%, 61 +/- 5%, or 38 +/- 6% of the controls when RPE cells were fed 40 mM D-glucose, 40 mM alpha MG, or 5 mM nonradioactive MI, respectively. Growth media containing either pyruvate or lactate, the glucose metabolites, did not suppress the ability of RPE cells to accumulate MI. An 18 +/- 8% reduction in [3H]thymidine incorporation into DNA occurred when cells were grown in 40 mM glucose for 12-14 days, compared to cells grown with 5 mM glucose. Chronic treatment (12-14 days) of the cells with phorbol ester, an activator of protein kinase C, caused up to twofold increase in MI uptake, [3H]phlorizin binding, cell number, and DNA synthesis. However, when the rates of MI uptake into cells grown in basic growth media or TPA-treated media were normalized to cell number, no significant difference in MI uptake was found between the treated and untreated cells. Addition of staurosporin, a protein kinase C inhibitor, together with TPA, in the growth media reversed the phorbol-induced increase of MI uptake.(ABSTRACT TRUNCATED AT 400 WORDS)

Alkaloids↗