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Biomedical subjects

M F Jayle

Publications and source records attributed to M F Jayle.

At least 55 records · Page 3Linked to original sources
PubMed1975

[Excretion of 7 estrogens during last days of human pregnancy].

The excretion of seven urinary estrogens was studied during the last two or three weeks of five normal pregnant women. A study of the urinary excretion curves demonstrated that 16 alpha-hydroxyestrone and 16-oxo-estradiol followed the same pattern with the particularity of presenting two excretion peaks which were persistently observed on the 8th or 7th day prior to delivery for the first one, and around the 4th or 3rd day prior to delivery for the second one. It is possible that there is a relation between this phenomenon and the onset of delivery.

Estrogens↗
PubMed1975

Surface behaviour of oestradiol-17beta.

The absorption of [3H]oestradiol-17beta from its aqueous solutions has been measured in the range 0-10mug/ml. It is found that the first adsorbed molecules are parallel to the interface and occupy 100 A2. Those adsorbed in the range 6-10 mug/ml occupy 21 A2. They are presumably associated. When the adsorption occurs in the presence of a synthetic lecithin monolayer, the molecular area is equal to 16 A2. Surface tension measurements of the solutions of oestradiol-17beta and a parallel study of their fluorescence have been performed. No association of the hormone molecules has been observed in bulk. It is concluded that surfaces and liquid monolayers may favour molecular association of the oestradiol-17beta.

Estradiol↗
PubMed1975

[An immunonephelometric determination of rabbit haptoglobin].

An immunonephelemetric determination of rabbit haptoglobin was carried out. At first, pure haptoglobin was isolated and then monospecific immunserums were obtained from rats. This method completes the regular determination of haptoglobin in all cases where the peroxydasic activity of the haptoglobin-hemoglobin complex is inhibited either by an hemolytic process, as is the case for rabbits, or by some inhibitors of the peroxydasic reaction.

Animals↗
PubMed1975

[Gamma-glutamyltransferase activity in the human red blood cell membrane (author's transl)].

A gamma-glutamyltransferase activity is found in the human red blood cell membrane. Membrane isolation was carried out according to the method of Dodge et al. (Dodge, J. T., Mitchell, C. and Hanahan, J. (1963) Arch. Biochem. Biophys. 100, 119-130) (modified) and proteins were solubilized either with 1% sodium deoxycholate or 5 mM EDTA or 10 mM of its disodium salt, under various conditions of time and temperature. The gamma-glutamyltransferase activity of the membrane preparations was investigated using two substrates, gamma-L-glutamyl-p-nitroanilide and gamma-L-glutamyl-alpha-naphthylamide. The specific enzymatic activities of the various preparations, expressed in m units per mg of protein, were found to have similar values under similar technical conditions. The chelating agents seem to allow a more specific isolation than the detergent. The presence of a gamma-glutamyltransferase activity in the erythrocyte membrane is discussed in relation to the membrane association of this enzyme in other tissues.

Cell Fractionation↗
PubMed1975

[Biosynthetis in vitro of estrogens by an adrenal cortex tumor].

A polysecretory cortico-adrenal tumor from a 46 year old woman was incubated in presence of tritiated pregnenolone and progesterone. Nine estrogens were isolated and identified by radio-gas-chromatography. This outstanding synthesis ability was in line with the high rates of urinary estrogens found in this patient.

Adrenal Cortex Neoplasms↗
PubMed1975

[Semi-automated determination of urinary 17-oxosteroids in a continuous flow system (author's transl)].

A semi-automated determination of 17-oxosteroids in urinary extracts is described using a modified Zimmerman reaction in aqueous phase according to Epstein. In order to eliminate the interfering chromogens a double manifold and a double-beam colorimeter are used in continuous flow. The spectra and the chromogenecity of seven different 17-oxosteroids are presented. The specificity and the reproducibility of this technique are good and the comparison with gas-liquid chromatography and manual Zimmerman reaction shows a good correlation.

17-Ketosteroids↗
PubMed1975

Gas chromatography profile of estrogens: application to pregnancy urine.

A method for the simultaneous quantitation of 7 estrogens in pregnancy urine is described. It involves enzymatic hydrolysis extraction of free steroids, ion-exchange column chromatography and quantitation of the trimethylsilyl derivatives by gas chromatography on OV 1. Data obtained from normal and twin pregnancies and from women with anencephalic fetus or intra uterine fetal death are analysed. The sensibility of the method is about 40 mug of each estrogen by liter of urine.

Chromatography, Gas↗
PubMed1975

Plasma diethylstilboestrol binding proteins of rat, mouse and man in the course of development: relations with the binding of estradiol.

High diethylstilboestrol (DES) binding has been demonstrated in fetal and adult sera from man, rat and mouse by equilibrium dialysis and electrophoretic techniques. In the adults of the three species and in the human fetus only albumin shows an elevated binding capacity for DES. By contrast, in the case of rat and mouse embryos there are two proteins, namely albumin and alpha-fetoprotein, which afford major and quantatively similar contributions to the binding. Human alpha-fetoprotein does not bind DES. These phenomena are analysed in relation to the estrogen binding characteristics of the alpha-fetoproteins of the three species.

Aging↗
PubMed1975

[Isolation and physical chemical properties of rat hemopexin].

Rat hemopexin was purified by a procedure involving three different steps : ammonium sulfate precipitation, rivanol precipitation and DEAE-cellulose chromatography with concave gradient of molarity. Purity of the preparation was checked by three different methods : analytical ultracentrifugation, immunoelectrophoresis and acrylamide gel electrophoresis. The principal physical properties were studied. The amino acid and carbohydrate composition was determined and compared with that of human and rabbit hemopexin.

Amino Acids↗
PubMed1975

The comparative specificity of 3 oestradiol-binding proteins. Rat alpha-foetoprotein, rat liver 17beta-hydroxy steroid dehydrogenase and anti-(oestradiol-6-carboxymethyloxime-bovine serum albumin) antiserum.

1. The specificity of 3 oestradiol-binding proteins was studied. Two of these proteins are naturally occurring (rat alpha-foetoprotein and rat liver microsomal 17beta-hydroxy steroid dehydrogenase) and the third is an artificially induced model, anti-(oestradiol-6-carboxymethyloxime-bovine serum albumin) gamma-globulins. 2. A specific binding procedure for each protein model permitted a determination of its affinity for oestradiol and for 30 other steroids. 3. The results obtained have brought to light the different areas of the steroid molecule that are important for its recognition by each of the three proteins. The two naturally occurring proteins (alpha-foetoprotein and 17beta-hydroxy steroid dehydrogenase) recognize the edge of the steroid defined by C-4, C-6, C-8 and C-15. On the other hand, the gamma-globulins recognize the opposite edge, i.e. that defined by C-2, C-10, C-11 and C-17. 4. Diethylstilboestrol, whose structure is analogous to that of a steroid, is only recognized by the two naturally occurring proteins.

Animals↗
PubMed1975

Biochemical criteria for the evaluation of drug efficiency on adjuvant arthritis and nephrotoxic serum nephritis in the rat: studies with phenylbutazone, L-Asparaginase, colchicine, lysine acetylsalicylate, and pyridinol carbamate.

The levels of serum orosomucoid, haptoglobin, and seromucoid were evaluated as possible quantitative criteria for the estimation of drug efficiency in adjuvant arthritis and nephrotoxic serum nephritis. In adjuvant arthritis, haptoglobin, seromucoid, and chiefly orosomucoid serum levels were generally very sensitive to anti-inflammatory agents such as phenylbutazone and pyridinol carbamate, and to immunosuppressive agents such as L-asparaginase. There was a significant correlation between the serum levels of these glycoproteins and the arthritis scores. In nephrotoxic serum nephritis, seromucoid levels were correlated with the proteinuria of the autologous phase and were found to be a good complementary criterion for the analysis of the efficiency of pyridinol carbamate, colchicine, iysine acetylsalicylate, and L-asparaginase.

Animals↗
PubMed1975

[Haptoglobin assay using the LKB 8600 reaction rate analyzer].

The reaction rate analyzer LKB 8 600 is used in a new haptoglobin evaluation method. This technique involves formation of peroxidatic complex between haptoglobin and horse or human hemoglobin (human hemoglobin is easier to prepare), then oxidation of gaiacol with registering of optical density rise. Results fit completely with those of the classical Technicon autoanalyzer technique. The method is accurate, fast, easy to perform, and appliable to large series, while necessitating only 10 mul of serum.

Animals↗
PubMed1975

[Secretion into the plasma of testosterone and estradiol 17 beta by the human testicle. Value of the study in male sterility].

The levels of oestradiol and of testosterone were estimated in the spermatic and in the perpheral veins in two series of 13 subjects. The one was a control group and the other stimulated with 10,000 International Units of chorionic gonadontrophir (II.C.G). The follwing conclusions can be drawn. --the first is that the testis secretes simultaneously testosterone and oestradiol 17 beta --the second is that the level of oestradiol is significantly raised after the administration of II.C.G. whereas the level of testosterone hardly alters. This leads on to the supposition that oestradiol does not arise from the same source in the testis as testosterone.

Chorionic Gonadotropin↗
PubMed1975

[Method of rapid separation of free, sulfoconjugated and glucuronoconjugated fractions of plasma steroids].

A method for the separation of serum conjugated steroids, sulfates and glucuronides, is described. It is based on the fact that methylene blue forms complexes with ester-sulfates which can be quantitatively and specifically extracted by chloroform from aqueous phase. The quantitative determination of the conjugated fractions thus obtained can be done after solvolysis followed by hydrolysis by beta-glucuronidase from Helix pomatia.

Chemical Fractionation↗