[Hemoglobin heterogeneity in rats].
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Animals↗
Biomedical subjects
M F Starodub
Publications and source records attributed to M F Starodub.
[Desoxyribonuclease activity of the spinal cord].
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Animals↗
[Immunosensors: sources of origin, achievements and perspectives].
The analysis of the recent data in the literature and results of investigations in the field of the development and study of function efficiency of different types of immune sensors, that are performed at the Department of Biochemistry Sensory and Regulatory Systems of the A. V. Palladin Institute of Biochemistry of Ukrainian National Academy of Sciences are presented. Sources of origination and perspectives of the devlopment of biosensors are discussed as well. The paper also gives an overview of main research projects at the Department, mainly in the filed of biosensors. They include development of the scientific bases for the creation of a new generation of chemo- and biosensors for their application in medicine and ecology. Multi-immune, multi-enzyme and combined multi-parametrical sensors can provide express analyses in laboratory and field conditions with the purpose to perform immune chemical diagnostics of diabet, kidney diseases, immune defficiencies, autoimmune, allergic, pre-infarction and pre-tumor states as well as to control total toxicity of the environment and identification of main types of toxic elements in it. The investigations are based on the latest achivements in the field of physics, chemistry, information technology and electronics with the use of different types of planar electrodes, ion sensitive field effect transistors (ISFETs), semiconductor capacitive structures, termistors, optrodes, piezocrystalls and application of such methods and effects as laser correlation spectroscopy, chemiluminescence, fluorescence, surface plasmon resonance, photoluminescence of porous silicon, interferometry, evanescent wave technique, nonemmiting energy transfer and holography.
Allergy and Immunology↗
[Enzymatic sensor for determing triglycerides].
An enzymatic sensor based on ion selective field effect transistors was developed for the determination of triacylglyceroles in the range of concentrations from 0.1 to 2 mM. It was show that the sensitivity of the sensor was equal to 0.1 mM and time of one measurement was 2 min. The optimal environment for the triacylglyceroles registration is 1 mM sodium phosphate buffer (pH 7.3).
Biosensing Techniques↗
[Thermobiosensors: features of design and function and prospects for their use].
Thermobiosensors are biosensors, which utilize thermal sensors (thermistors, thermocouple) for measuring changes of heat arising during ferment, immune or metabolic reactions. Thermosensors apply to an estimation of a condition of ferment systems, interactions of immune components, kinetics of a biocatalysis, and also for application in biochemical diagnostics, at definition of quality of products, monitoring of an environment and control of biotechnological processes. In the review the data, concerning of the major principle of development and creation mini- and microthermobiosensors, are given on the basis of modern achievement in the field of technology of micromanufacturing. The various types mini-, micro- and multithermobiosensors, and also their application in separate areas of the biochemical and clinical analysis and monitoring of an environment are surveyed. The development and modern achievement in creation of separate variants thermosensors is in detail described on the basis of thermistors and thermocouples. The place thermosensors in general development of biosensor controls is allocated and the attention to prospect of their further improvement and application is given.
Biosensing Techniques↗
[Immobilization of immunoglobulins using lectins with an immune sensor method based on surface plasmon resonance].
The effect of different lectins upon the response of immune sensor based on surface plasmon resonance (SPR) was investigated. Lectins affinity to carbohydrates of the IgG can be used to increase the density and orientation of IgG molecules at their immobilisation on the sensor surface. Conditions were elaborated for enhancement of immune sensor response in comparison with that one for bare or preliminary treated with dodecanthiol thin gold sensor surface. It was shown that human IgG revealed maximal affinity to wheat germ lectin and mouse monoclonal antibodies and rabbit IgG--to helix pomatia lectin. Pig antibodies, similar to human IgG, showed the greatest affinity to wheat germ lectin.
Animals↗
[Express diagnostics of bovine leucosis by immune sensor based on surface plasmon resonance].
An immune sensor based on the surface plasmon resonance (SPR) was developed for express diagnostics of bovine leucosis. The sensor was used for detection of the level of antibodies against bovine leukaemia virus (BLV) in the blood serum. The industrially manufactured BLV antigen for screening test in the agar gel immunodiffusion (AGID) required the additional purification in order to be used in immune sensor analysis. It was shown that immune sensor analysis was more sensitive, rapid and simple in comparison with the traditional AGID test. It was stated that the developed immune sensor was capable to be used for performance of bovine leucosis screening at the farms and the minimal dilution of the serum should be 1:500.
Agar↗
[Application of chemiluminescent technique for determination of Daphnia magna sensitivity to various types of toxic agents].
The character and intensity of activated chemiluminescence of Daphnia living medium are investigated depending on the effect on them of various concentration different in nature toxic substances, such, as potassium bichromate, methomyl and tween-80. It is shown, that the toxic influence of these agents can be defined by registration of stimulating effect of Daphnia's exometabolites on the process of chemiluminescence. The sensitivity of the method makes: for potassium chromate--0.005 mg/l, methomyl--0.0013 mg/l and tween-80--0.1 mg/l.
Animals↗
[The effect of hypoxic hypoxia on the physicochemical and functional properties of hemoglobin in rats].
The effect of intermittent altitude chamber hypoxia is established to cause an increase in solvability of laboratory rat hemoglobin. Results of immunochemical and fluorescent analysis of the samples of hemoglobin and its component are presented and discussed. They prove that changes in solvability of hemoglobin are determined by the conformational reconstructions of the respiration protein as a result of formation of the complexes with internally erythrocytic metabolites.
Adaptation, Physiological↗
[Disk electrophoresis of hemoglobin in polyacrylamide gel].
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Electrophoresis, Disc↗
[Development of enzyme biosensor based on trypsin and conductometric thin-film electrodes for protein and artificial substrates determination].
Two analytical systems based on the soluble and immobilized trypsin and conductometric thin-film electrodes were developed to measure concentration of artificial substrate and protein in solution. It was shown that these systems allow one to determine concentrations of Ha-benzoyl-L-arginin-ethyl-ester in the range of 0.1-1.0 mM and concentrations of HSA: 0.1-2.0 mg/ml with soluble and 0.1-0.8 mg/ml with immobilized trypsin.
Arginine↗