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Biomedical subjects

M F Zhang

Publications and source records attributed to M F Zhang.

32 records · Page 2Linked to original sources
PubMed1990

[Studies on sensor for L-ascorbic acid using biotissue slice].

Bioselective membrane electrode for L-ascorbic acid has been made with tissue slices. It is made from porcine liver tissue slice covering an oxygen electrode. The influence of buffer mediator, pH, temperature, antiseptics, slice thickness, etc. on the response characteristics of the electrode was examined. In the presence of copper ions, a linear relationship between dynamic response of the electrode and the logarithm of concentration of L-ascorbic acid was observed for the range of 21.14 micrograms/ml to 352.3 micrograms/ml, with a correlation coefficient of 0.9996 and the Michaelis constant of 0.8 mmol/L. The electrode has been used to determine L-ascorbic acid in drugs, the analytical results were satisfactory.

Animals↗
PubMed1989

Treatment of typhoid fever with ofloxacin.

Ofloxacin, 300 mg 12-hourly, was given orally to 64 patients with typhoid fever, all with positive blood cultures. Almost 80% of the isolates were resistant to chloramphenicol, ampicillin and co-trimoxazole, but sensitive to ofloxacin and norfloxacin. Fever subsided within five days in most patients (mean 3.2 +/- 1.1), rates of clinical effectiveness and bacteriological cure both being 100%. Thirty patients were followed for 1-3 months after the completion of therapy with no occurrence of relapse.

Adolescent↗
PubMed1989

A study on tea-pigment in prevention of atherosclerosis.

Since Lou Fu-qing et al first reported that tea had effect on the prevention of atherosclerosis (AS) in the 1960s, we have conducted experimental and clinical studies, and found that tea-pigment (TP) could reduce blood coagulability, increase fibrinolysis, prevent platelet adhesion and aggregation, and decrease cholesterol content in aortic walls in vivo (P less than 0.01). In addition, TP was shown to inhibit the proliferation of cultured human aortic smooth muscle cells in normal and elevated LDL circumstance in vitro (P less than 0.01), and decreased the level of T x B2 in platelet-rich plasma and remote myocardial infarction patients (P less than 0.01). TP can be used in the prevention of AS.

Animals↗
PubMed1989

[Efficacy of ofloxacin in treating typhoid fever].

We studied 75 patients (36 males and 39 females), suffering from typhoid fever. 64 patients were treated with ofloxacin and 11 with amikacin, dosage regimens of the two drugs were 300 mg and 300-400 mg twice daily. Clinical effective rate was 100% with ofloxacin and 36.4% with amikacin. A total of 72 strains of salmonella typhi was isolated from all the patients of both groups. Bacteriological elimination rate was 100% with ofloxacin after treatment. Sensitive rates for S. Typhi isolated was 100% with ofloxacin, norfloxacin and ceftriaxone, 98.6% with amikacin and 20-21.4% with chloramphenicol, ampicillin and sulfamethoxazole Co. There were fewer adverse reactions and better acceptance, one had skin rash and one had gastrointestinal disturbance. In amikacin group, abnormality of urine routine and serum creatinine was observed. Ofloxacin was well absorbed orally. Its high bioavailability, satisfactory therapeutic efficacy excellent tolerability and convenience for use make it a very useful medication in the therapy of typhoid fever resulted from multiresistant strains.

Adolescent↗
PubMed1989

[Antidiarrheal and anti-inflammatory effects of berberine].

Berberine sulfate (Ber) 40 and 80 mg/kg ig reduced the purging effects of castor oil or Cassia angustifolia leaf in mice, but did not affect the gastrointestinal transport function of Chinese ink in normal mice. Ber 60 mg/kg ig significantly inhibited the increased vascular permeability induced by ip 0.7% acetic acid in mice. Ber 20 and 50 mg/kg sc markedly inhibited the increased vascular permeability induced by histamine 100 micrograms/0.1 ml ic in rats. Ber 4 and 8 mg/kg sc produced obvious inhibition in the xylene-induced swelling of mouse ear. The anti-inflammatory effects were enhanced in a dose-dependent manner. It is suggested that the antidiarrheal effect of Ber is relative to its restriction against exudative inflammation to a certain extent.

Animals↗
PubMedDate not supplied

Enantiomeric determination of the phenylmorpholinol metabolite of bupropion in human plasma using coupled achiral-chiral liquid chromatography.

A coupled achiral-chiral stationary phase liquid chromatographic technique was developed to separate and quantitate the enantiomers of the phenylmorpholinol metabolite (2) of the antidepressant bupropion (1) in human plasma. At the retention time of 2, a switching valve loaded a portion of the eluting compound onto a protein-bonded chiral stationary phase which resolved 2 into the (+) and (-) stereoisomers using an aqueous mobile phase of potassium phosphate (pH = 6.25) and 5% 2-propanol. All eluting compounds were monitored using UV detection at 214 nm, and no plasma endogenous material or other commonly used psychotropic drugs were found to interfere. Within-day and between-day variation were less than 6% over the expected concentration range, and a limit of quantitation of about 125 ng/mL of 2 was observed. Steady-state plasma samples from 17 patients receiving 1 were found to contain the (-) enantiomer to the extent of about 96% of total 2. The potential clinical implications of these results are not known since all previous pharmacological studies were carried out with the racemic 2.

Antidepressive Agents, Second-Generation↗
PubMedDate not supplied

Sequence analysis of the VP2 hypervariable region of nine infectious bursal disease virus isolates from mainland China.

The VP2 hypervariable region of infectious bursal disease virus (IBDV) from nine Mainland Chinese strains was amplified by reverse transcriptase/nested polymerase chain reaction and cloned into pGEM-T vector. The nine isolates, which were from the center (HN3), the north (Bj-1, B2/28, HD96), the east (JS-18 and AH-2), the northeast (D11-2, C4-2), and the west (Ts) of China, were sequenced and compared with each other and with six reference IBDV sequences. Clustering analysis separated the nine isolate into two groups. The six virulent isolates, propagated in bursae, formed the first group. They revealed only one to three amino acid changes from the very virulent (vv) European and Japanese isolates, suggesting that they might have the same origin as European and Japanese vvIBDV strains. On the basis of their distinct geographic origins, extensive dissemination of vvIBDV in China was indicated. (The other three chicken embryo fibroblast cell cultured isolates with mild pathogenicity were placed in the second group.) Their sequences correlated closely with those of the culture-adapted strains (Cu-1 (4) and Cj-801). None of the nine isolates showed very close sequence relationship with the antigenic variant strains from the USA. Although antigenic variants have been reported in China, the reverse transcriptase/polymerase chain reaction-restriction endonuclease analyses of the nine viruses tested herein were not similar to any U.S.A. variant strains on the basis of computer software analysis. Our results and conclusions agree with a previous molecular study of IBDV isolates from the south of China.

Amino Acid Sequence↗