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M Fahnestock

Publications and source records attributed to M Fahnestock.

43 records · Page 3Linked to original sources
PubMed1988

The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex.

Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. Mastomys HMW NGF sediments as a 5S species, does not exhibit esterase activity, and is more difficult to dissociate at acid pH than mouse 7S NGF. The biological activity could be isolated as a purified Mastomys beta NGF protein identical in size and charge with that purified from male mice. The N-terminal amino acid sequence of the first 20 residues was determined and found to differ from that of mouse only at residue 8. Western blotting of Mastomys 5S NGF using antiserum against mouse beta NGF indicates that the beta NGF subunit of Mastomys is very similar to that of the mouse. Southern blots using a mouse kallikrein probe also demonstrate the presence of a large kallikrein family in Mastomys similar to that in mouse, and Northern blots verify transcription of kallikreins in Mastomys submaxillary gland. SDS-PAGE and isoelectric focusing gels reveal a Mastomys subunit that comigrates with mouse alpha subunit. However, neither oligonucleotide probes directed against mouse alpha subunit RNA nor antibodies directed against mouse alpha NGF cross-react strongly with the Mastomys material. This indicates that the second subunit of the Mastomys complex is not very similar to the mouse alpha subunit.

Animals↗
PubMed1988

The NGF and kallikrein genes of mouse, the African rat Mastomys natalensis and man: their distribution and mode of expression in the salivary gland.

The kallikrein genes and their expression in the salivary glands of mouse, the African rat Mastomys natalensis and human were compared. The Mastomys kallikrein genes comprise a family of genes similar to those of mouse. Androgen markedly enhances transcription of glandular nerve growth factor (NGF) and kallikrein in both male and female Mastomys suggesting the presence of testosterone regulated kallikrein genes for growth factor precursor-processing in both sexes. In contrast, although a kallikrein transcript was detected in human salivary glands of the same size as the mouse or Mastomys transcript no difference in the amount of transcript was seen in adult male or female. The absence of kallikrein genes regulated by testosterone and of NGF transcripts in the human implies that there is no human equivalent of the mouse salivary 7S NGF complex.

Animals↗
PubMed1987

Iodination of the progesterone receptor from hen oviduct spares the DNA-binding domain.

The progesterone receptor from hen oviduct is isolated as a complex of two subunits, A and B. The A protein binds one molecule of progesterone and also binds to DNA with high affinity. The native A protein can be labeled with iodine with no loss of DNA-binding activity. Limited Staphylococcus aureus V8 protease digestion of the labeled preparation results in a number of DNA-binding and non-DNA-binding fragments of the receptor. The progesterone-binding domain contains iodine label. However, two low-molecular-weight DNA-binding fragments do not contain iodine label, indicating a lack of susceptible tyrosine residues near the DNA-binding site of the native receptor. The labeled receptor and its fragments will facilitate studies of the isolated DNA-binding and progesterone-binding domains of the hen A protein as well as of the activity of the native receptor in the presence and absence of hormone.

Animals↗
PubMed1986

The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland.

Mouse submaxillary gland contains many proteolytic enzymes, the most widely studied of which are the kallikreins. This gland also contains high levels of nerve growth factor (NGF), which is isolated as a complex of three subunits, alpha, beta, and gamma. We report here the cloning and sequence analysis of a novel kallikrein from mouse submaxillary gland. Antibodies directed against the alpha subunit precipitate the product of this clone, but do not precipitate the homologous gamma subunit. This new kallikrein is therefore closely related to alpha NGF, yet in contrast to the alpha subunit, its sequence suggests it has proteolytic activity.

Amino Acid Sequence↗
PubMed1979

Control of the receptor for galactose taxis in Salmonella typhimurium.

The chemotactic response to galactose in wild-type Salmonella typhimurium is not inducible by galactose, but is inducible by fucose, a non-metabolizable analog. In a galactokinase mutant, however, the galactose receptor is inducible by galactose. These data indicate that the concentration of free galactose in the cell controls the levels of the galactose receptor. The intensities of the chemotactic responses were found to vary in proportion to the concentration of galactose receptors. In bacteria with higher levels of galactose receptors, the ribose response is inhibited by galactose. This supports the model in which the ribose and galactose receptors compete for a common component of the signaling system.

Chemotaxis↗