PubMed Health⌕ Search

Biomedical subjects

M Farriol

Publications and source records attributed to M Farriol.

At least 19 recordsLinked to original sources
PubMed1995

Protein synthesis in specific tissues during sepsis.

BACKGROUND: The hypothesis that fractional protein synthesis rates (Ks) are tissue-specific and bidirectional during sepsis was tested in an animal model. MATERIAL AND METHODS: Ks in liver, triceps muscle, and diaphragm were measured in septic (n = 27) and control rats (n = 26). Sepsis was induced by a reproducible model established in our laboratory (intraperitoneal injection of sterile NaOH 0.75 N at 0.075 ml/100 g of body weight). Ks were measured using the flooding-dose method in tissue obtained from the diaphragm, liver, and from the triceps muscle. RESULTS: In hepatic and diaphragmatic tissue, Ks were significantly higher in the septic animals (Ks: 112.2 +/- 8 and 5.4 +/- 1.9, respectively) than in control animals (Ks: 78.5 +/- 13 and 2.9 +/- 1.7, respectively). In the triceps, Ks were significantly lower in septic animals (Ks: 2.9 +/- 1.4) than in control animals (Ks: 5 +/- 1.8). CONCLUSION: The results suggest that in septic animals the rate of protein synthesis is enhanced in tissues of priority, such as the liver, and varies in response to differences in muscle activity.

Animals↗
PubMed1995

Addition of glutamine does not improve protein synthesis and jejunal mucosa morphology in non-hypercatabolic stress.

To investigate the effect of glutamine-enriched total parenteral nutrition (TPN) on the protein synthesis and morphology of jejunal mucosa in non-hypercatabolic stress, sixty-two male Sprague-Dawley rats were subjected to surgical stress by femoral fracture. The rats were divided into 3 groups and received TPN for 8 days. One group received a standard amino acid solution without glutamine, the second group a standard solution enriched with glycine and glutamic acid, and the third group a standard solution enriched with glycyl-glutamine. All regimens were isocaloric and isonitrogenous-nitrogen (2.2 g/kg.day), glucose (150 Kcal/kg.day), and lipids (150 Kcal/kg.day). There were no statistically significant differences in jejunal mucosal thickness, DNA content, protein content, fractional synthesis rate or absolute protein synthesis among the groups after eight days of parenteral nutrition. In conclusion, the addition of glutamine to TPN did not influence either protein metabolism or morphology of the jejunal mucosa in non-hypercatabolic surgical stress.

Animals↗
PubMed1994

Epidermal growth factor excretion in burned rats.

Evidence for epidermal growth factor (EGF) involvement in the physiological response to burns was sought from urinary levels of EGF, urea and creatinine in male rats using a standardized thermal skin injury model (25 per cent body surface) and treated with fluid resuscitation. Postmortem, the skin lesions were studied by microscopy to guarantee the absence of inflammatory complications. Statistically significant differences were observed in body weight, urea and creatinine excretion when compared to the basal values. When EGF excretion results were evaluated as raw data (expressed as ng/mg of creatinine or ng/day) compared to basal levels, no statistically significant differences were observed. However, when the results were expressed as percentage increases with respect to the basal values, a statistically significant increase was found over the first 7 days postinjury (P = 0.029).

Animals↗
PubMed1994

Effect of medium chain triglycerides (MCT) on jejunal mucosa mass and protein synthesis.

The effects of medium chain triglycerides (MCT) on jejunal mucosa mass and protein synthesis were compared with results from previous experiments with rats fed by parenteral nutrition or enteral nutrition. Other published studies have also been analysed. Three experimental models were studied. In the traumatic model, production of a femoral fracture was followed by Kirschner pin insertion into the medullary canal of both fragments at reduction. (Forty ras were fed enteral nutrition and 93 were given parenteral nutrition.) A second model entailed resection under ether anaesthesia using the technique described by Higgins. (Fifty five rats were fed enteral nutrition and 28 with parenteral nutrition.) A third model entailed a terminolateral portocaval shunt under anaesthesia with pentobarbital. (Sixty nine rats were treated this way and then given enteral nutrition.) Proportions of medium chain/long chain triglycerides (LCT) were as follows: 0/100, 20/80, 40/60, 50/50, and 92/8 for enteral nutrition and 0/100, 30/70, 50/50, and 70/30 for parenteral nutrition. Faecal losses of alpha amino nitrogen, protein, total fats, and free fatty acids were analysed together with the quantitative intake, weight gain of the rats, jejunal mucosal mass, and protein synthesis in relation to the MCT proportion ingested or given by enteral nutrition or parenteral nutrition. From analysis of our results and those of others, several conclusions could be drawn. Firstly, the route of administration of MCT is extremely important and enterocytes might be considered one of the main target sites. Secondly, a high proportion of MCT (more than 80%) offers no advantage for jejunal mucosa and produces undesirable side effects. Thirdly, the effect of MCT on jejunal mucosal protein synthesis depends on the metabolic state. Finally, an increase in jejunal mucosal mass directly correlated with MCT concentrations, but no correlation was found between mass and protein synthesis. A positive correlation, however, between MCT proportion and enzyme activity (alkaline phosphatase and sucrase) in the brush border membrane was seen as well as a positive correlation with the concentration of phospholipids in the microvilli.

Animals↗
PubMed1994

Effect of vitamin C and vitamin E analog on aged fibroblasts.

Human dermal fibroblasts were cultured and aged in vitro. Survival of young and aged fibroblasts was determined in the presence and absence of different concentrations of two vitamins. Vit C at doses of 5, 12.5, 25 and 50 mumol/L and water-soluble Vit E (Trolox) at 1, 5, 10 and 50 mg/L, were added 30 minutes before oxidative stress, consisting of exposure to 5 mM hydrogen peroxide for 30 minutes. A non-radioactive cell proliferation cytotoxicity assay (MTT) was used to determine the protective effect of the vitamins studied. Vit C produced a clear cytoprotective effect on aged cells over the entire range of doses applied. The protection provided by Vit E, was less pronounced.

Aging↗
PubMed1993

[The effect of sepsis on liver protein synthesis].

A study was made of the possible influence of sepsis on liver protein synthesis in septic and control rats, fed either parenterally (TPN) or with water and feed available "ad libitum". Liver protein synthesis was determined using the single massive dose method. In experiment I, the septic and control animals received TPN for twenty-four hours: in experiment II water and food was available to them "ad libitum". Twenty-four hours after the sepsis occurred, the animals were injected with a massive dose of 14C-labelled leucine, and slaughtered by decapitation. The septic animals with water and feed available "ad libitum" were fasted voluntarily without any intake. This group of fasted septic animals was the only one which lost weight. Liver protein synthesis increased in septic animals in comparison with the control animals, whether receiving TPN (112.2 +/- 8/78.5 +/- 13) or with "ad libitum" nutrition (97.2 +/- 15/60.8 +/- 8). The increased liver protein synthesis in the septic animals was modified (although with reduced significance) by parenteral nutrition (112.2 +/- 8/97.2 +/- 15). Our results suggest that sepsis increases liver protein synthesis, and that this protein synthesis may be enhanced by parenteral nutrition.

Animals↗
PubMed1993

Inhibition of visceral protein synthesis by certain amino acid supplements.

This study was focused on the effects produced by diets with different amino acid proportions on visceral protein synthesis (liver and jejunal mucosa). Eight groups of rats received an enteral modular diet supplemented with different amino acids (Group 1: Ile, Leu, Val; Group 2: Phe, Met, Gly; Group 3: Glu, Arg, Gly; Group 4: Gly, Group 5: Orn-Asp, Cys, Pro; Group 6: Lys, Ser, Thr; Group 7: Tyr, His, Ala). Group 8 was the control group. Rats were fed for four days. At the end of this period a flooding dose of 14C-Leu was injected and animals were killed. Liver and jejunal mucosa were removed and protein synthesis rate was determined. Results show a decreased liver protein synthesis in group fed with aromatic amino acids (53.8 +/- 8.4 vs. 88.6 +/- 12.1) and Glu-Arg (68.6 +/- 10.9). In jejunal mucosa there was a decrease of protein synthesis in groups fed with aromatic amino acids (98.7 +/- 16 vs 160.5 +/- 49). These changes seem to be related to the intracellular amino acid pool size and its influence on protein metabolism.

Amino Acids↗
PubMed1991

Effect of different MCT/LCT ratios on protein synthesis in injured rats fed parenterally.

The effect of the lipidic composition of the diet on the proteic synthesis of the male Sprague-Dawley rats fed parenterally for four days has been studied. All animals received identical nitrogen and caloric intake, but the ratio of medium to long-chain triglycerides (MCT/LCT) varied: group 1, control (0/100); group 2 (30/70); group 3 (50/50) and group 4 (70/30). Hepatic and jejunal protein synthesis were determined with L-(1-14C)-leucine. In groups 1, 3 and 4 muscle protein synthesis was measured with L-(3,4-3H)-phenylalanine. In liver, there were no significant differences in the fractional synthesis rate among the groups. In jejunum, the control group showed a higher fractional synthesis rate with statistically significant differences among the groups. In muscle, group 3 presented the highest fractional synthesis rate with statistically significant differences (p less than 0.05).

Animals↗
PubMed1990

Influence of fat emulsions in parenteral nutrition on visceral protein synthesis: study in hepatectomized rats.

The aim of this study was to evaluate the effect of total parenteral nutrition (TPN) with 100% long-chain triglyceride (LCT) (group A) and 50/50% medium/long-chain triglyceride (MCT/LCT) (group B) fat emulsions on visceral protein synthesis in 70% hepatectomized rats. The rats were fed TPN continuously for 7 days posthepatectomy. Protein synthesis was measured in the liver and jejunal mucosa with the flooding dose method. All rats received the same caloric and nitrogen intake, without statistically significant differences. The only difference between the two groups was the proportion of MCT to LCT in the diets. Hepatic and jejunal mucosa protein synthesis were significantly higher in group B, fed 50/50% MCT/LCT. These results suggest that fat emulsions with 50/50% MCT/LCT significantly enhance visceral protein synthesis after partial hepatectomy.

Animals↗
PubMed1987

Influence of the qualitative caloric composition of enteral diets on liver protein synthesis in normal and operated rats.

Protein synthesis L-(l-14C) leucine in liver was studied. Normal (n = 40) and operated (n = 40) Sprague-Dawley rats with an initial body weight of 161.18 +/- 12 g were divided into four groups (n = 10), respectively. Each group, subjected to the same conditions of temperature, light, noise and movement received a different commercial formula for human enteral nutrition and water for 8 days (normal rats) and six days (operated rats). All rats received the same caloric and protein intake per ml (l kcal; 6-7.10(-3) g protein), with no statistically significant differences among groups. The differences in diets were merely qualitative. The tolerance to all diets was similar, with rats attaining the expected weight. Losses of protein, alpha aminic nitrogen, total fat and non sterified fatty acid in faeces were negligible. In normal rats, different results in liver protein synthesis were obtained. No mass/synthesis rate correlation was found. Under these conditions it would appear that these results are due to the different diets used. In operated rats, a significantly different liver protein synthesis per gram of body weight was obtained, but not per gram of organ weight. These results suggest that the influence of the qualitative caloric intake, in the stress phase, is less than in the normal state.

Animals↗
PubMed1985

N tau-methylhistidine: inhibitor of hepatic protein synthesis?

Protein synthesis in isolated perfused rat livers has been studied. Aminoacids at physiological rat concentration (U-14C)-Leucine 0.065 muCi/ml and N Tau-Methylhistidine His (Tau-Me) were added to the perfusion medium. His (Tau-Me) from 0.01 to 2.0 mM causes a reduction between 40-60% in the rate of secreted proteins, independently of the His (Tau-Me) concentration.

Animals↗
PubMed1984

Valuation of protein metabolism and albumin in patients submitted to peripheral parenteral nutrition (PPN).

A protein breakdown study was carried out in ten post-operative patients, 24 hours after operation and after five days of peripheral parenteral nutrition; five of these patients were in a good nutritive condition (group A) and five were suffering from malnutrition and were critically ill (group B). Results indicate that the administration of amino-acids achieved a decrease in the degree of protein breakdown in the two groups under study, and also it was observed that better use was made of the nitrogen administered, particularly by the liver when the nitrogenous supply is completed with carbohydrates such as glucose. The difference obtained in values between the oxidized leucine and the leucine administered suggests that there is an endogenous donor pool, and the differences existing between breakdown and leucine oxidation values indicate that in group A, nourished solely with amino-acids, breakdown is lesser and oxidation greater than in group B, nourished with amino-acids and glucose, which suggests that there is more stimulation to re-use the amino-acids in this group.

Amino Acids↗
PubMed1983

Intralipid and free plasmatic tryptophan in vitro.

In an attempt to investigate the role of the lipidic emulsion Intralipid in the development of metabolic encephalopathy in a patient showing high free tryptophan levels, the relationship between lipidic emulsion and free tryptophan was examined in in vitro experiments. The addition of intralipid to normal serum produces an immediate increase in non-esterified fatty acids and a parallel rise in free tryptophan. Moreover, when serum with intralipid is incubated at 37 degrees C, the lipases release new non-esterified fatty acids and the free tryptophan increases proportionally. The non-esterified fatty acid content of intralipid was found to be 12 +/- 2 mEq X 1(-1). An inverse correlation was seen between free tryptophan and different serum albumin concentrations. It is concluded that intralipid causes an increase in free tryptophan levels. It is known that in vivo free tryptophan modulates 5-hydroxytryptamine synthesis and thus may be considered a possible causal agent for encephalopathy.

Bilirubin↗