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Biomedical subjects

M Filip

Publications and source records attributed to M Filip.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

Increased production of collagen in vivo by hepatocytes and nonparenchymal cells in rats with carbon tetrachloride-induced hepatic fibrosis.

We have shown, using the proline:ornithine dual label method, that in normal rats, hepatocytes contribute in vivo about 80 to 90% of the newly synthesized hepatic collagen. In order to quantify the contribution of hepatocytes and nonparenchymal cells to collagen synthesis in vivo in hepatic fibrogenesis, rats with CCl4-induced liver fibrosis were given [5(3H)]proline and [14C]ornithine intraperitoneally. About 80% of the 14C in albumin and transferrin was present as arginine, following conversion of [14C]ornithine via the urea cycle. In contrast to hepatocyte proteins, in nonparenchymal cells and serum a negligible percentage of the radioactivity was present as [14C]arginine. These combined findings indicate that, in spite of the hepatocellular damage, the labeling of hepatocyte proteins was efficient and specific, validating the use of the proline:ornithine method in this experimental model of hepatic fibrosis. We calculated the [3H]proline/[14C]arginine ratio in hepatic collagen (after correcting for the relative frequencies of amino acids) as a percentage of the same ratio in either albumin or transferrin, the index hepatocyte proteins. In this experimental model, during active fibrogenesis, both hepatocytes and nonparenchymal cells increase their production of collagen 2-fold when compared to normal animals, and hepatocytes produce the majority of the newly synthesized hepatic collagen.

Animals↗
PubMedDate not supplied

The anxiolytic-like effects of 5-hydroxytryptamine3 (5-HT3) receptor antagonists.

The effect of six 5-HT3 receptor antagonists: ondansetron (0.01-3 mg/kg ip), granisetron (0.01-1 mg/kg ip), zacopride (0.01-3 mg/kg ip), tropisetron (0.001-0.1 mg/kg ip), MDL 72222 (0.01-3 mg/kg ip) and DAU 6215 (0.01-3 mg/kg sc) were examined in the conflict drinking test (Vogel test) and in the elevated plus-maze test in rats. Ondansetron (0.1-0.3 or 1 mg/kg), zacopride (0.1-1 mg/kg) and tropisetron (0.01 mg/kg) increased the punished responding in the Vogel test and showed anxiolytic effects in the elevated plus-maze test. Their effects were limited to a narrow dose range and were not dose-dependent. Granisetron (0.1 mg/kg) exhibited an anti-conflict activity, but was ineffective in the elevated plus-maze test. MDL 72222 and DAU 6215 were ineffective in both those tests. On the other hand, diazepam (2.5-10 mg/kg), used as a reference drug, was active in either procedure and its effects were dose-dependent. These results indicate that an anxiolytic-like activity is not a common characteristic of 5-HT3 receptor antagonists. Moreover, even the anxiolytic action of drugs which were active in the experimental models used should be accepted with caution.

Animals↗
PubMedDate not supplied

[Sympathetic ophthalmia with an atypical onset].

Authors report the case of a patient in whom a sympathetic ophthalmia with posterior onset under the aspect of a massive CRSC was diagnosed. The clinical and therapeutic aspects are described in detail.

Adult↗
PubMedDate not supplied

The action of the couple ascorbic acid--Cu(II) on NAD(+)-coenzymatic function.

The coenzymatic capacity of NAD+ decreases by exposing it to the action of ascorbic acid--Cu(II) system, a generator of free radicals. The participation of the radicals in degrading NAD+ depends on the nature of the buffer, incubation medium pH, temperature, incubation time. Thiourea, IK, GSH, cysteine, mannitol, albumine reduce to different extents the noxious action of the radicals. The decrease of the coenzymatic function of NAD+ is caused by the break of esther and anhydride links, evidenced by releasing th cian-sensitive compounds--nicotinamide riboside and nicotinamide ribonucleotide. Also the presence of ortophosphate was demonstrated.

Ascorbic Acid↗
PubMedDate not supplied

[The reasons for failure in retinal detachment].

Detachment of the retina is still a very serious ocular disease which poses many problems to the ophthalmologist. The study shows the causes of the treatment failure in this disease on the basis of the authors' experience and the specially literature.

Adult↗
PubMedDate not supplied

[Pseudophakic glaucoma].

Pseudophakia generates a new pathology, sometimes atypically solved, insufficiently codified yet. The paper presents the experience on quite a large group of cataract-operated patients with implantation of pseudofac, in which cases of ocular hypertonia appeared.

Glaucoma↗
PubMedDate not supplied

[Etiology, therapeutic management and functional results in traumatic cataract in children. A 5-year study].

The paper reports on the author's own experience in the surgical treatment of the traumatic cataract in children. The authors consider that implantation of the artificial crystalline must not be a general implantation method, in any eye. It has to be made discriminatingly and take into consideration the posttraumatic inflammatory reactions and the local state of each eye.

Adolescent↗
PubMedDate not supplied

[The action of a fibric acid derivative on lipid metabolism].

The action of N-2 (p-chlorophenoxy isobutiril)-N-morpholino-methylureea on some serum lipids parameters in rats with normolipemia and tritonic hyperlipidemia was studied. In the animals with normolipidemia, the compound induces hypocholesterolemia beginning with the 5th day of treatment, hypotriglyceridemia during the entire investigation interval and an increase of phospholipid levels in the 9th day of treatment. In the rats with tritonic hyperlipidemia, the substance induces hypocholesterolemia, hypotriglyceridemia, increase of HDL-cholesterol levels, decrease of (VLDL + LDL)--cholesterol levels and the return to normal values of Glueck ratio.

Animals↗
PubMedDate not supplied

[The cutaneous bioavailability of alpha-chymotrypsin from ointments].

In view of improving the cutaneous bioavailability of alpha-chymotrypsin from ointments, the stability of this enzyme in two hydrophile ointment bases, macrogoli and carbopol, was tested. The cutaneous bioavailability of alpha-chymotrypsin in ointments, estimated by determining the anti-inflammatory action, underlines the important role played both by the ointment base and by the enzyme activating substances. By correlating the determinations of alpha-chymotrypsin enzymatic activity with the anti-inflammatory action a correlation between the manifested activity and the active substance stability was noticed.

Animals↗