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Biomedical subjects

M Franke

Publications and source records attributed to M Franke.

At least 55 records · Page 3Linked to original sources
PubMed1987

Results of a phase-II clinical trial on treatment of rheumatoid arthritis with recombinant interferon-gamma.

In an open, non-randomized clinical trial conducted at multiple centres, 49 patients with rheumatoid arthritis were treated with recombinant interferon-gamma for 20 days. The study was carried out in two sub-studies. In the first, the total daily dose of interferon-gamma was 50 micrograms; in the second, 100 micrograms. Of the 49 cases, 40 were evaluable for statistical analysis; 24 of these patients (60%) responded to therapy, according to the criteria of a successful outcome laid down in the study protocol, and were classified as responders. In responders, the clinical parameters investigated improved with both dosages. The lower dosage differed from the higher one in having a markedly lower incidence of side-effects. The results lead to the conclusion that a randomized double-blind phase-III clinical trial should be performed.

Adult↗
PubMed1987

Azodisalicylate (azodisal sodium) causes intestinal secretion. Comparative study of the effect of azodisalicylate, sulfasalazine, 5-aminosalicylic acid and sulfapyridine on the water and electrolyte transfer and the morphology of the rat ileum and colon in vivo.

Azodisalicylate (ADS) is one of the possible successors of sulfasalazine in the treatment of ulcerative colitis. The following results were obtained when comparing the influence of ADS on net water and electrolyte transfer in tied-off loops of the rat ileum and colon in vivo with sulfsalazine, 5-aminosalicylic acid, and sulfapyridine. (1) Sulfasalazine, 5-aminosalicylic acid and sulfapyridine had no effect on water and electrolyte transfer of the healthy intestinal mucosa in concentrations up to 400 mg%. (2) ADS showed a concentration-dependent inhibition effect on net water, sodium and chloride absorption and stimulated secretion at concentrations higher than 100 mg%. (3) No morphological alterations of the mucosa could be observed by light and transmission electron microscopy. The observed effect of ADS might have some clinical significance in patients with a decreased absorptive capacity of the colon in ulcerative colitis, in contrast to healthy volunteers, where the high absorptive capacity of the colon might compensate the decreased absorption or stimulated secretion in the small intestine.

Aminosalicylic Acids↗
PubMed1986

[A test for the detection of fibrin in the plasma].

The article reports on measurements of D dimer, a terminal plasmic lysis product of crosslinked fibrin, with an enzyme immunoassay (ELISA) employing recently developed specific monoclonal antibodies. Due to its sensitivity the test can be used on plasma samples. The D dimer concentrations in patients with deep vein thrombosis diagnosed by laboratory apparatus were significantly increased compared to a control group; in one patient with additional pulmonary embolism, the level was even higher. Moderately elevated concentrations of D dimer were observed in the hypercoagulable state of pregnancy, puerperium and during the postoperative course. This reduces the specificity of the test with regard to the recognition of thromboembolic episodes under these conditions. Obstetric patients with disseminated intravascular coagulation (DIC) showed excessively increased levels of D dimer. Hence, a marker function with regard to the recognition of thromboembolic disease can be attributed to the D dimer; the diagnosis of DIC can be confirmed if very high concentrations are detected.

Adult↗
PubMed1986

Characterisation of nucleolar proteins as autoantigens using human autoimmune sera.

Sera from 271 patients with systemic rheumatic diseases were screened for antinucleolar antibodies using immunofluorescence. Antinucleolar antibodies were found in the sera of 73% of patients with progressive systemic sclerosis, 4% of patients positive for autoantibodies with rheumatoid arthritis, and 8% of patients with rheumatoid arthritis associated with sicca complex, but not in patients with systemic lupus erythematosus, Sjögren's syndrome, undifferentiated connective tissue disease, or in healthy donors. The antinucleolar sera (n = 20) were analysed by immunoblotting techniques. In four sera antibodies against nucleolar proteins with molecular weights of 35 kD, 37 kD, 69 kD, 92-93 kD, and 93 kD could be immunodetected. The nucleolar autoantigens were extractable from the nucleoli together with the preribosomal particle fraction and could be solubilised by ribonuclease (RNAse) treatment. Their presence in the nucleolus was sensitive to actinomycin D treatment of cells. The 37 kD autoantigen could be identified as nucleolar phosphoprotein B23.

Animals↗
PubMed1986

[Renal dipeptidylpeptidase IV excretion in drug-induced kidney changes].

The course of the excretion of dipeptidyl-peptidase IV (DP IV)-alanine aminopeptidase, beta-glucuronidase and total protein with the urine was investigated during the treatment of 11 patients with pyelonephritis with gentamicin, after application of a renal radiographic contrast medium in 7 patients with arterial hypertension and after regional perfusion of an extremity in 10 patients with malignant melanoma. In the reference group in male test persons with 147.0 nmol/s X l a higher DP IV activity in the urine was recognized than in the female test persons (100.0 nmol/s X l). After application of the drugs a rhythmically intermitting increased excretion of all enzymes mentioned develops. The study confirms the usuability of the DP IV-activity for enzymological investigations of the urine.

Acute Kidney Injury↗
PubMed1982

Enzyme immunoassay of human urinary kallikrein. Determination of human urinary kallikrein, III.

An enzyme immunoassay for the determination of human urinary kallikrein has been developed and is compared with other human urinary kallikrein assays such as radioimmunoassay, dog blood pressure assay, rat uterus test after kinin liberation and synthetic substrate(20) tests (AcPheArgOEt and S-2266). The usable range of the standard curve is from 0.05 to 12 ng kallikrein per test. The intraassay coefficient of variation is between 2 and 4%, the interassay coefficient of variation is between 4 and 12%, and the recovery of authentic kallikrein added to urine samples is 95%. Human saliva and human pancreatic kallikrein show the same binding curves as purified human urinary kellikrein. Kallikrein from urine of rats, dogs and rabbits as well as boar acrosin and pig pancreatic kallikrein, bovine trypsin and chymotrypsin show no cross-reactivity.

Animals↗
PubMed1982

[A contribution to the standardization of the diagnosis and therapy of sarcoidosis I and II].

UNLABELLED: 485 cases of sarcoidosis of a hospital for lung diseases within the time from 1960 to 1971 were retrospectively studied by examining, if diagnostics and therapy till now practised are sufficient. In most cases diagnosis was due to clinical signs only in cases of doubt biopsy was used. The standard treatment with 750 mg Prednisolon was carried one. The course of disease and the results of therapy were chequed by documents and radiography. By a consultation of the Chest Clinics charged with the care after therapy the results were completed showing that the degree of right diagnosis and therapeutic effects were positive. RESULTS: diagnostics suffice (histological diagnosis: only 13.9% of the cases), 6 wrong diagnoses (1.31%), but not fatal. 27.56% recidivs, which equal those of higher and longer methods of therapy, therefore, the paper is a contribution to the rationalization of the therapy.

Adult↗
PubMed1980

[Inhibition of propionibacteria in the sebaceous gland duct using photochemotherapy and 8-methoxypsoralen].

It was shown by in vitro studies that 8-methoxypsoralen (= 8 MOP) alone and UVA irradiation alone produces relatively little inhibition of the growth of Propionibacterium acnes and Propionibacterium granulosum, whereas a combination leads to an intense suppression of bacterial growth. It was further demonstrated by in vivo studies on 14 subjects that local treatment with 8 MOP and UVA significantly reduces the propionibacteria in the pilosebaceous duct. These results afford a possible explanation for the well known favorable effect of photochemotherapy on acne vulgaris. In addition, they contribute to explaining the drier appearance of the skin after photochemotherapy.

Acne Vulgaris↗
PubMed1978

Effect of topical application of dexamethasone on Propionibacteria in the pilosebaceous duct.

A preparation of dexamethasone 0.02% in Eutanol G (n octyldodecanol) was applied once daily for three weeks, to the right side of the forehead of 25 male healthy test subjects. Eutanol G without dexamethasone was applied in the same way to the left side of the forehead. Before and at the end of the treatment period bacteria were removed from the pilo-sebaceous ducts by the method of Holland et al. [8]. Bacteria that grew under anaerobic conditions were evaluated quantitatively. Dexamethasone induced a significant increase in P. acnes, in all Propionibacteria and in the total bacterial count. The importance of these findings is discussed in relation to the development of steroid acne.

Administration, Topical↗
PubMed1978

On the propionibacteria in the pilosebaceous ducts of uninvolved skin of acne patients.

In 24 persons with a severe inflammatory acne and 48 control subjects, bacteria were sampled from the sebaceous gland exretory ducts with the glass sampling head method according to Holland et al. After anaerobic culture bacterial counts were performed. The total numbers of P. acnes and the frequency of detection of P. granulosum were determined. The bacterial counts of P. acnes were almost idendical in the two collectives. P. granulosum was detected at a significantly higher frequence in the acne patients than in the control subjects. It can be assumed on the basis of the results that an increase in the bacterial counts of P. acnes is not to be regarded as a pathogenetic factor in the inflammatory acne of older patients. On the other hand, they suggest that P. granulosum may have an appreciable role in acne.

Acne Vulgaris↗