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Biomedical subjects

M Fukaya

Publications and source records attributed to M Fukaya.

At least 55 records · Page 3Linked to original sources
PubMed1989

Nucleotide sequence of the membrane-bound aldehyde dehydrogenase gene from Acetobacter polyoxogenes.

The nucleotide sequence of the membrane-bound aldehyde dehydrogenase (ALDH) gene from an industrial vinegar producer, Acetobacter polyoxogenes, was determined. Comparison of the sequence with the NH2-terminal amino acid sequence of the mature ALDH and determination of the actual translational initiation codon by means of in vitro manipulation of the upstream and proximal regions of the cloned gene showed that ALDH was primarily translated as a 773-amino-acid protein and that the 44-amino-acid sequence at the NH2-terminus, which probably serves as a signal peptide, was processed during maturation and localization in the membrane. When ALDH was expressed in a large quantity in Escherichia coli cells after the coding region had been placed downstream of the lac promoter, the ALDH protein, which still contained the signal peptide and had no ALDH activity, was localized in the membrane fraction.

Acetobacter↗
PubMed1989

Cloning of the Membrane-Bound Aldehyde Dehydrogenase Gene of Acetobacter polyoxogenes and Improvement of Acetic Acid Production by Use of the Cloned Gene.

A genomic clone bank of Acetobacter polyoxogenes NBI1028 constructed in Escherichia coli by use of the expression vector pUC18 was screened with antibody raised against membrane-bound aldehyde dehydrogenase (ALDH; 75 kilodaltons [kDa]) from A. polyoxogenes NBI1028. A clone that synthesized a 41-kDa protein cross-reactive with anti-ALDH antibody was isolated. For cloning of the full-length ALDH structural gene, a cosmid gene bank was screened by Southern blot hybridization with the cloned DNA as a probe, and subcloning from the positive cosmid clone was performed with shuttle vector pMV24. Plasmid pAL25, containing the full-length ALDH structural gene, was isolated and expressed in both E. coli and Acetobacter aceti to produce a fused protein (78 kDa) with a short NH(2)-terminal beta-galactosidase peptide. pAL25 conferred ALDH production on a mutant of A. aceti lacking the enzyme activity. Transformation of A. aceti subsp. xylinum NBI2099 with pAL25 caused 2- and 1.4-fold increases in the production rate and in the maximum concentration of acetic acid in submerged fermentation, respectively.

Journal Article↗
PubMed1989

[A case of lupus pneumonia treated by pulse therapies and plasma exchange therapy].

A case of interstitial pneumonia accompanying SLE (lupus pneumonia) was reported. Shortness of breath in this patient was aggravating very rapidly even during methyl prednisolone pulse therapy, but the patient was relieved from this symptom soon after double filtration plasma exchange was carried out. We concluded that some cases which are resistant to pulse therapy and whose prognosis is very poor might be saved by plasma exchange.

Betamethasone↗
PubMed1989

[Salivary calculus of the parotid gland: report of a case].

We reported a case of parotid sialolithiasis. A man, aged 72, complained of swelling of the left buccal region accompanied by a sensation of inflammation. On diagnosis of sialolithiasis of the parotid gland, a surgical operation was performed with a small incision in the left cheek region. About one month after the operation, a salivary calculus was discharged spontaneously from the left buccal region. He recovered satisfactory without the formation of a parotid salivary fistula. Morphological observation on the salivary calculus was carried out using the scanning electron microscope (SEM).

Aged↗
PubMed1989

Effects of Nd: YAG low power laser irradiation on the ulnar nerve.

In our previous study, we have shown that low power laser irradiation of the stellate ganglion produces a rise in temperature in the bilateral facial skin. To clarify the mechanism behind this phenomenon, irradiation was performed on the ulnar nerve at the elbow joint. The sensation felt during irradiation was reported by the subjects. Changes in temperature of the hand dorsum were thermographically recorded. The evoked peripheral autonomic surface potentials (PASP) were also recorded. 77.3% of the subjects reported sensation changes in the hand; the temperature rose in both hands in 3 out of 5 subjects. The PASPs did not show marked changes in latency or amplitude. It is suggested that the effects of the irradiation are brought about mainly through the stimulation of the central nervous system.

Action Potentials↗
PubMed1989

Effect on the action potential of the low power Nd: YAG laser as irradiated directly to the nerve.

The present paper reports our studies of changes in the compound action potential after Nd: YAG laser irradiation of the rat's sciatic nerve. The nerve on one side was exposed and irradiated by Nd: YAG laser directly. The temperature of the irradiated nerve was also measured. 1) Under 100 mW irradiation the compound action potential and the temperature of the surface of the sciatic nerve did not show a detectable change. 2) Under 300 mW irradiation the compound action potential showed an increase in amplitude, and shortening in latency. A negative after-potential appeared within 30 secs of irradiation. The temperature on the sciatic nerve rose by 3 degrees C during the irradiation. 3) Under 500 mW irradiation the compound action potential decreased gradually in amplitude and ultimately disappeared. The temperature on the sciatic nerve reached more than 47 degrees C.

Action Potentials↗
PubMed1989

Holoprosencephaly: report of a case.

This is a case report of a patient suffering from holoprosencephaly associated with median cleft lip and palate who was treated in our clinic. The present patient was introduced to our hospital at the age of three months. We suspected holoprosencephaly on the basis of the peculiar facial structure. Lip plasty was performed because the holoprosencephaly of the patient would be of the least severe type capable of surviving a long time, and because of the parents' concern about the esthetic aspect. The modified Tennison's method was used. The patient is under follow-up observation at our hospital.

Abnormalities, Multiple↗
PubMed1989

Structural analysis of phleboliths and salivary calculi.

Three phleboliths and ten salivary calculi in the submandibular duct were studied with scanning electron microscopy (SEM) and computer aided microanalyser (CMA). The surface of the phleboliths was rather even with some irregularity. According to their surface structure the salivary calculi were divided into three types: the rock-like type, the granular type, and the globular type. The phleboliths were classified into two types according to their cut surface: the calcified-core type, and the uncalcified-core type. The core structure was similar to the surface structure in the calcified-core type of phleboliths, but in the salivary calculi the core structure was different from the surface structure in that the cut surface of the core was an accumulation of circular or polygonal structures forming a honey-comb pattern which was surrounded by small projections distributed radially. On the basis of these results some etiological factors of phleboliths and salivary calculi are discussed.

Electron Probe Microanalysis↗
PubMed1987

A clinicopathological study of ameloblastomas.

72 cases of ameloblastoma were obtained from the files of the Department of Pathology, School of Dentistry, Aichi-Gakuin University for the years January 1970 through December 1983. The cases were analyzed with respect to sex, age, duration, site histopathology, and treatment. Of 72 patients, 63 had no previous therapy, while 9 received their initial treatment elsewhere. There were 38 males and 34 females, a ratio of 1.2: 1. At the time of diagnosis, the ages of all patients ranged from 11 to 71 years, with an average of 36.6 years. About 65% of patients were in the 2nd, 3rd, and 4th decades of life. The duration of symptoms varied from 2 days to 5 years, with an average of 12.6 months. 69 cases occurred in the mandible, with the molar-ramus region being the most frequent site of involvement. Only 3 were found in the maxilla. The left side of the mandible was affected 1.6 times more frequently than the right. Histopathologically, 44 cases were of the plexiform type, 15 the follicular, 10 the acanthomatous, and 2 the basal cell type. Only 1 case was of the granular cell type. Most of the findings in the present study agreed with previous available data from the literature on ameloblastomas.

Adolescent↗