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Biomedical subjects

M Fuke

Publications and source records attributed to M Fuke.

At least 37 records · Page 2Linked to original sources
PubMed1977

Fidelity of ribosomal ribonucleic acid synthesis by nucleoli and nucleolar chromatin.

Isolated nucleoli, nucleolar chromatin, and nucleolar DNA were used as templates for DNA synthesis in appropriately supplemented systems in which RNA polymerases other than RNA polymerase I were blocked by alpha-amanitin. With the aid of nucleotide analysis, DNA-RNA hybridization, and homochromatography fingerprinting, it was found that isolated nucleoli and nucleolar chromatin serve primarily as templates for synthesis of rRNA. However, the products formed with purified nucleolar DNA as a template do not contain the specific rRNA oligonucleotides nor are they appreciably hybridized to the rDNA region on cesium chloride gradients. These results indicate that whole nucleoli and nucleolar chromatin contain control mechanisms that restrict readouts by RNA polymerase I of nucleolar DNA to rDNA.

Animals↗
PubMed1977

Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.

The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucleotide kinase and gamma-(32p)-ATP. The (32p)-labeled oligoribonucleotide is subjected to partial endonucleolytic digestion and fractionated by two-dimensional homochromatography fingerprinting. The nucleotide sequence is determined by following mobility shifts of the labeled and partially digested oligoribonucleotides in homochromatography fingerprinting.

Animals↗
PubMed1976

Evolutionary trends in 18S ribosomal RNA nucleotide sequences of rat, mouse, hamster and man.

The large T1 ribonuclease fragments of 18S ribosomal RNA from four mammalian species, rat, mouse, hamster and man, were compared by two-dimensional homochromatography fingerprinting. The nucleotide sequences of the large T1 ribonuclease fragments, polypyrimidines and polypurines which were different among the four mammalian species were determined and compared. The method used for determining nucleotide sequences utilizes 32p-labeling of oligonucleotides at their 5'-termini by polynucleotide kinase, partial digestion by ribonucleases and analysis of labeled spots by homochromatography-fingerprinting. Several examples of point mutations were detected. It was of interest that the 18S rRNA of Chinese hamster has more oligonucleotide sequences in common with those of man that rat or mouse.

Animals↗
PubMed1976

Allocation of 15 RNase T1-resistant large oligonucleotides of MS2 RNA.

Four large MS2 RNA fragments with the original 5'-end were obtained by limited RNase T1 digestion. The lengths of the fragments were 36, 30, 22, and 19% of the whole molecule. Whole MS2 RNA and the four fragments were completely digested with RNase T1 and analyzed by the two-dimensional "homochromatography fingerprint" technique. Sixteen oligonucleotides of MS2 RNA were separated and the oligonucleotides were assigned to the fragments. Fifteen of the sixteen oligonucleotides could be classified into three regions of the cistrons of bacteriophage MS2.

Base Sequence↗
PubMed1972

Electron microscopic studies of replicating and catenated colicin factor E1 DNA isolated from minicells (DNA replication).

Replicating and catenated DNA molecules of colicin factor E1 that were isolated from minicells were observed by electron microscopy. Two new replicating structures that have been found include: molecules that contain two untwisted replicated branches of the same length and a twisted unreplicated branch, and a twisted circular molecule with a long tail. Other molecular forms identified include circular dimers, three types of catenated dimers (open-open, open-twisted, and twisted-twisted), catenated dimers in which one of the linked molecules is replicating, catenated trimers, and a tetamer.

Centrifugation, Density Gradient↗
PubMed1970

Replicating DNA: structure of colicin factor E1.

Replicating circular molecules of colicin factor E1 have been isolated from minicells produced by a colicinogenic derivative of Escherichia coli P678-54. The replicating structures observed in electron micrographs are circular molecules with two branched points. Several structures found among the replicating molecules are interpretable as replicating rolling circles.

Colicins↗