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Biomedical subjects

M Furmanowa

Publications and source records attributed to M Furmanowa.

18 recordsLinked to original sources
PubMed2004

Immunomodulatory effect of shikonin derivatives isolated from Lithospermum canescens on cellular and humoral immunity in Balb/c mice.

The immunomodulatory activity of acetylshikonin (ACS) and isobutyrylshikonin (IBS) was studied in female and male inbred Balb/c mice, and in F1 hybrids (Balb/c x C3H). ACS and IBS were isolated from Lithospermum canescens Lehm. (Boraginaceae) roots. Splenocytes from mice fed 40 microg of ACS had higher proliferative potential in cultures with PHA than corresponding controls and also higher migratory in vitro activity than splenocytes obtained from control animals. ACS at a 40 microg daily dose stimulated G-v-H reaction but inhibited it at a 200 microg dose. IBS at a 40 microg dose significantly increased humoral response.

Adjuvants, Immunologic↗
PubMed2003

Detrimental effects of water extracts from surface and interior of Taxus baccata leaves on the two-spotted spider mite (Tetranychus urticae Koch).

Surface deposits on Taxus baccata needles removed by dipping in water of 96, 60 or 40 degrees C for 5 s caused changes in life history components of mites. Paclitaxel was among other peaks present in the removed fractions in concentrations between 0.017 and 0.170 microg/g of fresh weight (f.w.) increasing with temperature. Long extraction for 60 min at only 40 degrees C did not increase removable paclitaxel, but at 60 degrees C extraction rate was the highest (1.326 microg/g) suggesting that leakage from an interior of needles occurred. Mortality, developmental time, total fecundity, oviposition period and life history parameters of Tetranychus urticae Koch. were detrimentally affected.

Animals↗
PubMed2002

Antimicrobial activity of Polyscias filicifolia cell biomass extracts.

Antibacterial activity of extracts of Polyscias filicifolia biomass from bioreactor and callus was determined using the agar disc-diffusion method. The microorganisms Staphylococcus aureus (three strains) showed the highest sensitivity to extracts of P. filicifolia biomass from a bioreactor. The values were comparable with nitrofurantoine used as a standard. Micrococcus flavus, Sreptococcus pyogenes and S. agalatiae were less sensitive. The effect of P. filicifolia callus extract on the above bacteria was less pronounced than that of extracts of biomass from a bioreactor.

Anti-Bacterial Agents↗
PubMed2001

In vitro propagation of Withania somnifera and isolation of withanolides with immunosuppressive activity.

Withania somnifera plantlets were produced in vitro from the shoot-tip of aseptically germinated seedlings. Culture conditions were optimized using different plant growth regulators which gave rise to 120 shoots from a single bud. The plantlets were then transferred to pots and maintained in greenhouse for 4 months. 90% of these in vitro propagated plantlets survived and showed normal growth. Leaves from these plants were used for isolation of the withanolides. Methanolic extract of leaves from plantlets growing in tissue culture and those transferred to the greenhouse were evaluated for immunomodulatory activity. While the extract from greenhouse samples showed potent immunosuppressive activity, those from tissue cultures samples did not show any activity. Fractionation and characterization of withanolides, using HPLC, NMR, MS methods revealed the presence of withaferin A in the greenhouse samples. Our results indicate that Withania species may require longer time and better differentiation and also natural environment for the production of withaferin A.

Adjuvants, Immunologic↗
PubMed2000

Polyprenols in hairy roots of Coluria geoides.

Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75% (w/v) glucose and at 22 degrees C. Dry samples of roots were extracted and lipid content was analysed by HPLC. According to our estimation, polyprenols are accumulated in roots of C. geoides cultivated in vitro as a mixture of several prenologues with the dominating prenol composed of 16 isoprenoid units. The content of polyprenols in tissue was approx. 300 microg/g of dry weight.

Culture Techniques↗
PubMed1996

Sample preparation for taxol and cephalomannine determination in various organs of Taxus sp.

Solid-phase extraction and preparative thin-layer chromatography were applied as sample preparation techniques for the purification of crude extracts from twigs and needles of various Taxus species as well as for the isolation of taxol and cephalomannine for further reversed phase high performance liquid chromatography analysis. Significant differences in the contents of taxanes examined were found. The preparative chromatographic methods used were compared and evaluated as routine and reproducible procedures for the rapid isolation and determination of taxol and cephalomannine in plant extracts.

Antineoplastic Agents, Phytogenic↗
PubMed1992

[Searching for cytostatic substances in plant tissue of Maytenus molina by in vitro culture. III. Release of substances from active biological fractions from the callus extract of Maytenus wallichiana R. and B].

Chromatographic studies controlled by biological evaluation (Tetrahymena test) showed some colour substances among them tingenone and beta-sitosterol. In order to identify the substances extraction and biologically controlled chromatography was carried out. Two substances were isolated, their properties corresponding with tingenone and beta-sitosterol.

Microbial Sensitivity Tests↗
PubMed1990

[Investigating cytostatic substances in tissue of plants Maytenus Molina in in-vitro cultures. II. chromatographic test of extracts from callus of Maytenus wallichiana R. et B].

Maytenus wallichiana callus tissue was extracted. The extract was fractionated to achieve maytansine containing residue. In none of the fractions maytansine corresponding substance was found. However evaluation by Tetrahymena test indicated strong cytotoxicity (ID50 1.8 micrograms/cm3). Chromatography of biologically active fractions demonstrated the presence of colour chinone methide triterpenes (tingenone).

Antineoplastic Agents↗
PubMed1989

[The search for cytostatic substances in the tissues of plants of the genus Maytenus molina in in vitro culture. I. Callas culture and biological studies of its extracts].

Plant tissue cultures of Maytenus wallichiana Raju et Babu and Maytenus emarginata Ding Hou were initiated. Growth conditions of the callus and the optimum medium composition have been established. Increments of callus wet mass and dynamics of callus growth were determined. Morphological and microscopic observations were also performed. The most efficient growth of the callus, resulting in increments of its wet mass up to 6460%, was obtained on the modified Murashige and Skoog medium. Extracts of the callus were found to be inactive against microorganisms, but proved cytotoxic for lymphocytic leukaemia L 5178Y (ED50 18-48 micrograms/cm3) and the protozoon T. pyriformis (ID50 43-53 micrograms/cm3).

Animals↗
PubMed1983

Mutagenic effects of aqueous extracts of Symphytum officinale L. and of its alkaloidal fractions.

Symphytum officinale L. (Boraginaceae) is a medicinal plant widely used in therapy. It roots, described in the Polish Pharmacopoeia as Radix symphyti, are recommended as expectorants, especially for children. Aqueous solutions of three alkaloid fractions obtained from infusions of Symphytum officinale L. root were tested for their antimitotic and mutagenic activity in meristematic cells of the lateral roots of Vicia faba L., var minor. Lasiocarpine, a proven carcinogen, served as a positive control. Mutagenic effects were induced by lasiocarpine, by the alkaloidal fraction I and by diluted infusions from Radix symphyti. Fraction III had only antimitotic effect. The biological activity of the tested solutions is discussed in relation to the relevant literature.

Alkaloids↗
PubMed1979

[Not Available].

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Botany↗