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M Furuki

Publications and source records attributed to M Furuki.

7 recordsLinked to original sources

Femtosecond near-field scanning optical microscopy study of molecular thin films.

A near-field scanning optical microscope has been combined with a two-colour time-resolved pump-probe measurement system. It has a noise-equivalent transmittance change of 5.0 x 10-5 for a probe pulse with an intensity of 30 nW. The system has been used for evaluating molecular thin films that have a domain structure, particularly for observing a gate action of the single domains. The results include key features to understand an origin of the domains and suggest that the film composition is uniform over a distance of several micrometres.

Journal Article↗

A missense mutation of the gene for Na+,K(+)-ATPase alpha-subunit causes abnormal feeding behavior in Caenorhabditis elegans.

The Na+,K(+)-ATPase activity of membranes from a behavioral mutant of C. elegans was found to be about one-third that of the wild-type. The levels of mRNA and polypeptide of Na+,K(+)-ATPase alpha-subunit in the mutant were as high as those in the wild-type, but the level of the phosphorylated intermediate of the Na+,K(+)-ATPase in the mutant worm was 80% lower than that in the wild-type. A single predicted amino acid replacement (Leu to Phe) was found in a highly conserved region in the alpha-subunit that is involved in the formation of phosphorylated intermediate. The abnormal feeding behavior of the mutant worm may be attributed to this missense mutation.

Amino Acid Sequence↗

Cloning, characterization and functional analysis of groEL-like gene from thermophilic cyanobacterium Synechococcus vulcanus, which does not form an operon with groES.

A gene encoding 57 102 Da polypeptide homologous to groEL of Escherichia coli but accompanying no groES, has been cloned and sequenced from a thermophilic cyanobacterium, Synechococcus vulcanus. The amount of the gene transcript increased several folds by heat shock. The gene was expressed as a minor component of two types of HSP60, and designated as groEL2. Although expressed and induced well upon heat shock treatment in the E. coli, introduction of the cloned groEL2 gene of S. vulcanus into an E. coli groEL-less mutant did not result in the complementation of heat sensitivity.

Amino Acid Sequence↗

Interferometric measurement of femtosecond optical pulses emitted from a fibre probe.

An interferometric technique is applied to a temporal analysis of the far-field radiation from a near-field optical fibre probe. A balanced homodyne scheme combined with lock-in detection at dual harmonics allows one to evaluate the temporal form of weak signal pulses, even in the presence of some environmental vibrations that disturb the optical interference. The transmission properties of a small aperture are formulated theoretically. It is shown that the sub-wavelength aperture should not distort the femtosecond optical pulses temporally, which is consistent with the experimental observation.

Journal Article↗