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M Furusawa

Publications and source records attributed to M Furusawa.

At least 109 records · Page 6Linked to original sources
PubMed1987

A study of familial non-polypotic colorectal cancer.

The family history of colorectal cancer was examined in 601 patients with non-polypotic colorectal cancer. 33 (5.5 per cent) of these patients were found to have a family history. Colorectal cancer was found significantly more often in the families of those patients with the disease rather than in those with gastric cancer (5.5 per cent vs 3.0 per cent). The mean age was younger by five years in the patients with a family history than in those with no family history. The incidence of young patients, multiple cancers, and other cancers in relatives was significantly higher in the patients with a family history than in those without. No significant difference in cancer sites between the two groups was seen, however, there was a preponderance of right colon cancers in the young patients with a family history. There was one particular patient with seven affected relatives in his family. In this patient, an early age of onset, the presence of multiple cancers, including a right colon cancer, the occurrence of metachronous other cancers and the fact that one of his relatives was accidentally found to have an occult colon cancer were noteworthy. It is important to survey the family members of patients, especially the young ones, where there is a family history of colorectal cancer.

Adult↗
PubMed1987

Hyperproduction of polyhedrin-IGF II fusion protein in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrosis virus.

A gene coding for insulin-like growth factor II (IGF II) was constructed from 16 oligodeoxynucleotides synthesized chemically and cloned into EcoRI-SalI sites of pBR322. In this gene at ATG codon for methionine was introduced for cleavage by CNBr at the beginning of mature IGF II. For expressing foreign genes, a new host-vector system, with Bombyx mori silkworm larvae as the host and B. mori nuclear polyhedrosis virus (BmNPV) as the vector, has been developed. BmNPV genomic DNA codes polyhedrin which is a major protein of inclusion bodies and is mass-produced in infected silkworm larvae. We employed this polyhedrin production system to obtain a large yield of a foreign gene product. The coding region of the carboxy-terminal half of polyhedrin was removed and the remainder was ligated with the IGF II gene in phase to create a fusion protein gene consisting of the coding region of the amino-terminal half of polyhedrin and the IGF II gene. This fusion protein gene was combined in a plasmid with the promoter and 5' and 3' flanking regions of the polyhedrin gene. The resulting plasmid and the wild-type BmNPV genomic DNA were cotransfected into BM-N cells, and a recombinant virus was isolated by the limiting dilution method. The silkworm larvae infected with the recombinant virus produced 3.6 mg of the fusion protein per larva and the infected BM-N cells produced 0.3 mg per ml of culture. IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed.

Animals↗
PubMed1987

Lymphotoxin activity of regional lymph nodes of cancer patients and its relation to stages of cancer and the regulatory role of macrophages.

Lymphotoxin (LT) activity of regional lymph nodes from patients with uterine cervical cancer and patients with gastric cancer was studied. LT release from PHA-stimulated lymph node cells was decreased slightly in stage I of uterine cervical cancer and gastric cancer. Decrease of LT release was pronounced in advanced stages of both types of cancer. Spontaneous LT release by unstimulated lymph node cells was high in 8 of 20 internal iliac node of uterine cervical cancer in stage I. However, LT release was decreased in advanced stages. Similar tendency was observed in gastric cancer. Relation of LT release to the activity of macrophages was examined. Reverse correlation was observed between LT release and the cytostatic activity of adherent cells. This result suggests that macrophages play a regulatory role on LT production and release by regional lymph nodes in human cancer.

Adult↗
PubMed1987

[Intraoperative radiotherapy].

Intraoperative radiotherapy, which delivers a large single dose of electrons to a tumor intraoperatively, is applied for locally advanced and/or difficult to cure malignancies which are rather difficult to treat by conventional external beams. Although this method was initially developed in Japan, increasing numbers of institutes have recently been working with IORT on a world-wide basis because of the increased availability of medical accelerators and the better results obtained. The malignancies for which IORT is indicated are cancers of the stomach, liver, biliary tract, pancreas, colo-rectum, urinary bladder, prostate and uterus, which are surrounded by normal tissues or organs with low tolerance doses, and also recurrent or difficult to cure malignancies of the brain, lung, mediastinum, bone and soft tissues are becoming candidates for IORT treatment.

Female↗
PubMed1986

Local recurrence after curative anterior resection for carcinoma of the rectum.

From 1972 to 1985, 60 patients with rectal carcinoma underwent curative anterior resections. There was a local recurrence in three (5 per cent). Pelvic recurrence was seen in two patients and there was a suture line recurrence in one. The length of the distal margin of the normal bowel or lymph node involvement did not appear to be a significant determining factor of the local recurrence. As for the factor responsible for the recurrence, tumor implantation into the pelvic cavity or into the suture line was suspected. Care should be taken to avoid implantation of tumor cells during operative procedures. There were no local recurrences in patients with lower rectal carcinoma. This finding might be related to the selection of patients with carcinoma of stage I and II.

Adenocarcinoma↗
PubMed1986

Significance of measurement of a carbohydrate antigenic determinant 19-9 (CA 19-9) for monitoring colorectal cancer patients.

The significance of measurement of serum CA 19-9 levels and the correlation between the serum levels of CA 19-9 and CEA in 61 patients with colorectal cancer were investigated. CA 19-9 levels above 37 U/ml were found in 24.6 per cent, while CEA levels above 5 ng/ml were observed in 34.4 per cent. Elevation of one or both of two antigens was found in 44.3 per cent. In patients with elevated CA 19-9 levels, the CA 19-9 levels returned to normal after curative resections, whereas patients with normal levels of this antigen showed only slight or no differences after curative resections. Measurements of this antigen were useful for the evaluation of the effect of surgery, especially in patients with normal CEA levels and elevated CA 19-9 levels. Combination assays of these antigens may be more useful than CEA alone for monitoring colorectal cancer patients.

Antigens, Neoplasm↗
PubMed1986

Macrophage activity of regional lymph nodes of cancer patients and its relation to stages of cancer.

Macrophage activity of regional lymph nodes from patients with uterine cervical cancer and patients with gastric cancer was studied. Enhanced spreading of macrophages was observed in an early stage of these cancer patients, but the rate was decreased in advanced stage. In metastatic nodes, enhanced spreading was observed in many cases. Cytostatic activity of macrophages was elevated in stage II of uterine cervical cancer and in stage I of gastric cancer. Significant relation between the rate of spreading and the cytostatic activity was observed in these patients. The reasons for change of the macrophage activity in relation to tumor burden were discussed.

Adult↗
PubMed1985

Sexual and urinary dysfunction following surgery for sigmoid colon cancer.

We investigated sexual and urinary dysfunction following surgery for sigmoid colon cancer. Thirteen (46.4 per cent) of the 28 male patients with a normal sexual life prior to surgery could no longer ejaculate, but all could maintain erection. With the extension of lymph node dissection, the incidence of a disappearance of ejaculation tended to increase. In particular, in patients undergoing an extended lymph node dissection, the incidence was 53.8 per cent. Urinary dysfunction occurred in 7 (10.3 per cent) of the 68 patients (37 men and 31 women), but was slight to mild. There were no significant differences between the extent of lymph node dissection and urinary dysfunction. In surgery for sigmoid colon cancer, care should be taken to preserve the hypogastric nerves.

Adult↗
PubMed1985

Comparative studies on (2'-5')oligoadenylate-related enzyme systems and the antiviral effect of interferon in two mouse cell lines which differ in (2'-5')oligoadenylate sensitivity of their protein synthesizing system.

Effect of (2'-5')oligoadenylate (2-5A) on cellular and viral protein and RNA syntheses was investigated with two mouse cell lines, L929 and Lz (a subclone of L929). The oligonucleotide was introduced into the cells either by using calcium phosphate coprecipitation technique or by microinjection method. In L929 cells protein and viral RNA syntheses were severely inhibited by 2-5A, whereas in Lz cells, both were only slightly inhibited. The activities of 2-5A synthetase and double-stranded (ds)RNA-dependent protein kinase were enhanced by interferon (IFN) treatment roughly to the same extent and there was no significant difference in the level of 2'-5' phosphodiesterase activity either. On the other hand, 2-5A-dependent RNase (RNase L) activity in Lz cells was low, being about 10-20% of that of L929 cells. It was increased twofold after IFN treatment, but protein synthesis of Lz cells was not as sensitive to 2-5A as that of L929 cells even after IFN treatment. L929 and Lz cells were sensitive to the antiviral effect of mouse IFN against vesicular stomatitis virus (VSV) and Mengovirus. In contrast, however, Lz cells were relatively insensitive to the antiviral effect of IFN on vaccinia virus, whereas L929 cells were sensitive.

2',5'-Oligoadenylate Synthetase↗
PubMed1985

Tubular adenoma with focal squamous metaplasia of the ascending colon.

A case of tubular adenoma with focal squamous metaplasia of the ascending colon in a 66-year-old male is reported. The tumor was a pedunculated polyp with a size of 1.5 X 1.4 X 0.9 cm. Histologically, the tumor showed tubular adenoma with moderate to severe atypia, was accompanied by focal squamous metaplasia. It is thought that squamous epithelial polyp may be derived from colonic adenoma with squamous metaplasia, and that the malignant change of colonic adenoma with squamous metaplasia may lead to squamous cell carcinoma, adenosquamous carcinoma, and mucoepidermoid carcinoma. Only seven cases of colonic adenoma with squamous metaplasia have been reported previously in the literature, and it is yet necessary to discuss the histogenesis, significance, and natural history of colonic adenoma with squamous metaplasia.

Aged↗
PubMed1985

[Radiotherapy of pancreatic carcinoma].

During the 10-year period between 1974 and 1984, we treated 34 pancreatic carcinomas by radiotherapy as follows: Eight by intraoperative radiotherapy (IOR), five by external radiotherapy (ERT), and 21 by IOR in combination with ERT. The mean survival time of the eight patients treated by IOR was 5.6 months, and that of the 21 receiving the combination therapy 7.2 months. Although it is not statistically significant, patients treated by the combined regimen survived longer than those treated by IOR alone.

Aged↗
PubMed1984

Levamisole in postoperative adjuvant immunochemotherapy for gastric cancer. A randomized controlled study of the MMC + Tegafur regimen with or without levamisole. Report I.

The usefulness of LMS in postoperative immunochemotherapy of gastric cancer was investigated. In compliance with the protocol, MMC was given at a dose of 20 mg on the day of gastrectomy, and an additional 10 mg on the next day IV. The patients receiving 600 mg Tegafur daily were then divided into two groups according to whether LMS was also given or not. LMS was administered for 3 days before the operation in a daily dose of 150 mg and for 1 year or more after operation according to a schedule of 3 days' administration followed by an 11-day interval. The 2-year follow-up demonstrated that in stage III patients, the LMS (+) regimen was superior to the LMS (-) regimen, since the former prolonged the relapse-free interval significantly. The survival rate for stage III disease was also significantly higher in the LMS (+) than in the LMS (-) group. There was no significant difference in the incidence of subjective or objective side-effects between two groups. The incidence of agranulocytosis was comparable in the two groups.

Adult↗