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M G Gallo

Publications and source records attributed to M G Gallo.

29 records · Page 2Linked to original sources
PubMed1982

The morphologic pathway of exocytosis of the vesicular stomatitis virus G protein in cultured fibroblasts.

Cultured fibroblasts were infected with vesicular stomatitis virus (VSV) and the pathway of exocytosis of G protein, the transmembrane glycoprotein of VSV, was followed by immunofluorescence and electron microscopy. G protein was detected within the endoplasmic reticulum, within smooth vesicles and stacks in the Golgi region and on the cell surface. No G protein was detected in the coated regions of the Golgi. Our data are consistent with the hypothesis that coated regions of the Golgi are involved in transfer of lysosomal enzymes and other substances to lysosomes and not in exocytosis.

Animals↗
PubMed1981

Intracellular localization of actin in cultured fibroblasts by electron microscopic immunocytochemistry.

Antibodies to skeletal muscle actin were produced in rabbits and purified by affinity chromatography. Direct labeling of SDS-PAGE gels of whole cell homogenates from mouse fibroblast cells showed that actin was the only protein detected by these antibodies. Using this immunospecific reagent, we localized actin in cultured fibroblasts using the EGS fixation-permeabilization procedure with the ferritin bridge labeling technique. Swiss 3T3-4 mouse fibroblasts were chosen as an example of highly adherent untransformed cells with prominent microfilament bundles, and L929 mouse fibroblasts were chosen as an example of poorly adherent, rounded, transformed cells with prominent microvilli. Using these two cell types, we have characterized the intracellular distribution of action. Actin was only detected in locations in which morphologically recognizable 60 A microfilaments were found. By both fluorescence and electron microscopy, actin was found in surface ruffles, microvilli, microfilament bundles, the microfilament mat, and the leading lamellae of Swiss 3T3 and L929 cells. In addition, actin was found surrounding micropinosomes and macropinosomes. On the other hand, there was no actin detected around the base of coated pits. Morphometric quantitation showed that almost all the actin was localized in microfilamentous structures. Our results suggest that actin has an important role in cell motility and adhesion, and in the endocytosis of pinosomes, but that actin may not be involved in intracellular processes such as saltatory motion of intracellular organelles.

Actins↗
PubMed1981

Receptor-mediated endocytosis in cultured fibroblasts: cryptic coated pits and the formation of receptosomes.

Concentrative receptor-mediated endocytosis of many specific ligands by cultured fibroblasts occurs through the coated pit-receptosome pathway. The formation of receptosomes was studied using two impermeant electron-dense labels for the cell surface, ruthenium red and concanavalin A-horseradish peroxidase. These studies show that at 4 degrees C, virtually all coated structures near the plasma membrane are in communication with the cell surface, and are not isolated coated vesicles. On warming cells to 37 degrees C for only 1 minute, a major portion of these structures become cryptic, that is, not labeled by these surface markers. However, on cooling cells immediately back to 4 degrees C, virtually all of these structures are again in communication with the surface. Many images showed that membrane of these cryptic pits to be continuous with the cell surface when caught in the appropriate plane of section; often there was a very narrow entrance that excluded extracellular label. At 37 degrees C, receptosomes could be occasionally seen forming as an invagination of membrane adjacent to the coated region. Mechanisms by which receptosomes may form and other evidence demonstrating the failure of coated pits to pinch off to form isolated coated vesicles during endocytosis are discussed.

Animals↗
PubMed1978

[Dissemination of Fasciola Hepatica in cattle of Novara Province].

The diffusion of F. hepatica in the cattle of the Novara Province was studied by means of a necroscopic survey on 2184 regularly slaughtered animals and a copro-microscopic survey on 5063 feaces samples picked up from 162 breedings farms. The 28.40% of the slaughtered animals resulted infected with F. hepatica. The parasite also resulted present in 52.74% of the examined breeding farms. A large diffusion of Paramphistomum sp. was shown, too. The diffusion areas of these Trematodes may be seen in the figures 1, 3, 6 and 7.

Animals↗
PubMedDate not supplied

Dermatophytes from cases of skin disease in cats and dogs in Turin, Italy.

In urban environments, pet animals such as dogs and cats may be largely responsible for the progressive increase of zoophilic over anthropophilic dermatophytes in the aetiology of human dermatophytoses. Mapping, in each city, of the natural foci of zoophilic dermatophytes may thus be important for understanding the epidemiology of human dermatophytoses, and for planning preventive measures. We have surveyed dogs and cats suspected of carrying dermatophytic lesions in the Turin area. Hairs, skin scrapings and the contents of pustules or vesicles were inoculated on Dermasel agar supplemented with choramphenicol and cycloheximide. The mating type of the isolates was checked, testing their compatibility with the two mating types of Arthroderma simii Stockdale, Mackenzie and Austwick. We isolated dermatophytes from about 40% of the cases examined. Microsporum canis Bodin was the only dermatophyte found in the lesions. In the cats the lesions were more frequent, in the dogs more severe. Animals less than a year old and male dogs were most often affected. Some cases of transmission of the infection between animals and from animals to man are described. All the isolates of M. canis, tested for mating behaviour, were non-reactive.

Age Factors↗
PubMedDate not supplied

[Seasonal variations in microfilarema and effects of ambient temperature in dogs parasitized by Dirofilaria repens].

1 - Dogs infected with Dirofilaria repens from Italy were found to show seasonal variations in the number of microfilariae in the peripheral blood. 2 - The rise in the number of microfilariae occured in August and September and is coincidental with the higher probability of trasmission. 3 - Rise and fall of microfilaraemia was induced experimentally by exposure of the host to high and low temperatures respectively. 4 - The importance of such variations in microfilaraemia is stressed in relation to the evaluation of the infection from a clinical, pharmacological and epizoological point of view.

Animals↗
PubMedDate not supplied

[Presence of pathogenic yeasts in the feces of the semi-domesticated pigeon (Columba livia, Gmelin 1789, urban type) from the city of Turin].

The results of a survey on the presence of pathogenic yeasts in pigeon droppings collected in Turin, are shown. The study was carried out in 8 densely populated areas, where human-animal contact is highest. A total of 427 pigeon dropping samples, most of which fresh, were collected. 550 yeast colonies, clinically interesting or at least pathologically significant, were isolated by the identification routine methods. The yeasts belong to the following species: Candida albicans, C. humicola, C. krusei, C. guillermondii, C. lypolitica, C. lambica, C. parapsilosis, C. tropicalis, C. rugosa, C. zeylanoides, Cryptococcus albidus, C. laurentii, C. neoformans, Hansenula anomala, Geotrichum sp., Kloekera apiculata, Rhodotorula glutinis, R. rubra, Saccharomyces cerevisiae, Torulopsis candida, T. glabrata, Trichosporon beigelii, T. capitatum, T. cutaneum, T. pullulans.

Animals↗
PubMedDate not supplied

[Distribution of nematode parasites of the digestive system in sheep (Ovis aries) and goats (Capra hircus) of the Piedmontese and Valdostano Alpine arc].

A survey, carried out on gastro-intestinal nematodes of sheep and goats of Piemonte and of Valle d'Aosta (87 sheep and 12 goats) has shown the presence of the following species in sheep, Bunostomum trigonocephalum, Chabertia ovina, Cooperia curticei, Haemonchus contortus, Marshallagia marshalli, Nematodirus abnormalis, Nematodirus filicollis, Nematodirus helvetianus, Nematodirus spathiger, Oesophagostomum venulosum, Ostertagia circumcincta, Ostertagia lyrata, Ostertagia trifurcata, Skrjabinema ovis, Trichostrongylus axei, Trichostronglus colubriformis, Trichostronglus vitrinus, Trichuris ovis and Trichuris skrjabini; in goats, Bunostomum trigonocephalum, Chabertia ovina, Haemonchus contortus, Nematodirus filicollis, Nematodirus helvetianus, Oesophagostomum venulosum, Ostertagia circumcincta, Ostertagia ostertagi, Ostertagia trifurcata, Trichostrongylus axei, Trichostrongylus colubriformis and Trichostrongylus vitrinus. The percentage of each species in the two host is given in the text table.

Animals↗
PubMedDate not supplied

[Diffusion of helminth parasites of the digestive system and respiratory system in deer (Cervus elaphus) of the La Mandria Regional Park (Piedmont)].

A study was carried out on the diffusion of helminth parasites of liver, gastrointestinal system and bronchial system in 68 red deer (Cervus elaphus) from Regional Park "La Mandria" (Piedmont, Italy). The following species of nematodes were identified: Trichostrongylus axei, Spiculopteragia spiculoptera, Rinadia mathevossiani, Cooperia oncophora, Cooperia pectinata, Cooperia punctata, Cooperia zurnabada, Oesophagostomum venulosum, Dictyocaulus eckerti, Varestrongylus sagittatus. Studies on some nematodes, belonging to the genera Spiculopteragia and Ostertagia, are still in progress. A large diffusion of Fascioloides magna was also present (only two young specimens resulted negative).

Animals↗