PubMed Health⌕ Search

Biomedical subjects

M G Heulin

Publications and source records attributed to M G Heulin.

16 recordsLinked to original sources
PubMed1990

[Evolution of in vivo and in vitro tests during specific immunotherapy against hymenoptera venoms].

In this study, on a population of 26 patients with systemic reactions after hymenoptera stings, different immunobiological tests have been used to confirm the sensitivity to hymenoptera venom. These 26 patients were divided into two groups according to the stage of their immunotherapy: group 1 (n = 8) included all assessments from day 0 (before IT) and group 2 (n = 16) the assessments at 1 and 2 years during IT. In general, there was a good correlation between skin tests, RAST and histamine release. The results show that the IgE level seems to be more informative than histamine release and that measurement of the specific IgE level is more sensitive with Allerbio allergen compared with other allergens. However these preliminary results must be confirmed on a statistically significant number of patients. HL by Agf is less sensitive than that done with Ags.

Bee Venoms↗
PubMed1989

[Significance of anti-gluten IgA levels for detection and monitoring of celiac disease].

Diagnosis of coeliac disease rests in jejunal biopsy. This is recognised as a traumatic examination which today may perhaps be replaced by a biological test produced by Pharmacia in which the anti-gluten IgA is measured by the ELISA method. The sensitivity of the test reaches 100% and the specificity to 96%. This biological test makes it possible to follow the development of the coeliac illness under diet, since the anti-gluten IgA diminishes significantly, often to the normal level.

Adult↗
PubMed1989

[Role of food allergy in atopic dermatitis].

Food allergy is one of the main causes of atopic dermatitis. Its frequency was established at 41.3% in our study. Eggs, cows milk and wheat were the foods most often responsible for DA. Proof by elimination-reintroduction is the best diagnostic criterion in food allergy, leading to suppression of the food if its nutritional importance permits this. This food suppression leads to 84% clinical improvement of DA.

Adolescent↗
PubMed1988

[Antihistaminic or anti-degranulating activity of pregnancy serum].

From observation of women who had anaphylactic accidents to hymenoptera venoms, with positive histamine release, we have noticed that these same patients lose their histamine release during pregnancy. Measurement of histamine release to pollens confirms the existence of a histaminase, which develops from the third month of the pregnancy and which is the origin of the loss of histamine release. This study makes eliminates a possible anti-degranulation ability of pregnancy sera.

Amine Oxidase (Copper-Containing)↗
PubMed1988

[Comparative study of ELISA and RIA technics for the detection of specific IgG4].

The assay of IgG4 was done by an ELISA method that has been perfected in our laboratory over two years, or by the RIA (Pharmacia technique). Assay of IgG4 is an objective element in the control of specific immunotherapy (IT) to hymenoptera venoms and grass pollens. There is an excellent correlation between the two techniques of ELISA and RIA, that shows an increase in IgG4 titre after around 3 to 4 years of IT.

Antibody Specificity↗
PubMed1988

[Local and general experimental anti-anaphylactic effect of 554 L, a synthetic antihistaminic].

554 L, a synthetic histamine depressant, was first made in September 1956. In spite of the age of this compound, it seemed to us that it may be of interest to examine it for possible experimental general and localised bronchial antianaphylactic activity. In the first experiments, the results showed an anti-anaphylactic effect in guinea pigs that had been sensitised to cow milk. The second study proved that there was an anti-anaphylactic effect locally on the bronchii by an aerosol of 554 L or Hypostamine on guinea pigs that were sensitive to OVA. These results may produce development of this old compound in delayed release and aerosol presentations.

Animals↗
PubMed1988

[Cell-mediated immunity using anti-influenza vaccine (AIV) and an immunostimulant (R)].

21 infected asthmatics were involved in this study, divided into three groups: Group 1 (G1): 7 subjects who received Ribomunyl. Group 2 (G2): 7 subjects who received anti-influenza vaccine, replaced on the 8th day by Ribomunyl. Group 3 (G3): 7 subjects who received the anti-influenza vaccine. Every patient had a IMC assessment on days 0, 8 and 30 that was composed of: 1. An RE test to show the Ly T11 receptors. 2. A study of the lymphocyte sub-populations CD3, CD4 and CD8, that respectively exhibit the LyT, T-helper and T-suppressor receptors. 3. Lymphocyte function was studied by stimulation of the lymphocytes by lectins, PHA (Ly T), Con A (Ly Ts) PKWM (Ly 8): by TB co-operation). 4. Measurement of total IgE. Only the lymphocyte stimulation tests by lectins showed a significantly raised response in the subjects of G1, who received Ribomunyl. IAV and Ribomunyl (G2) or IAV alone (G3) showed lymphocytes responses that were statistically less than G1. It seemed as though the IAV retarded the Ribomunyl response in IMC. The RE test and the study of the sub-populations did not show any significant differences between the groups. No differences between the total IgE levels could be seen in the groups. This study indicates the possibilities in the treatment of super-infected asthmatics with Ribomunyl alone, since the anti-influenza vaccine had a tendency to retard cell-mediated immunity.

Adjuvants, Immunologic↗
PubMed1988

[Cell-mediated immunity in antibiotic therapy].

Should antibiotic therapy required in the treatment of acute infection be administered frequently in patients at risk with clinical conditions involving recurrent superinfection? The results of our ongoing investigations initiated in 1967 and the present study indicate that macrolides, particularly erythromycin depress the host defense system. In contrast in a study performed in subjects treated for 8 days using cell-immunity tests, we found that cefaclor and amoxicillin may have an enhancing effect on host defenses.

Amoxicillin↗
PubMed1988

[Determination of specific IgE by chemiluminescence. The IgE DHS-CLA system].

The DHS-CLA IgE system (MAST immunosystems) allows simultaneous determination of specific IgE for 35 different allergens. The method here described is compared with RAST and results are in good agreement with allergic etiology. CLA testing appears to be a good alternative when anamnesis does not permit a clear diagnostic orientation.

Animals↗
PubMed1987

[Determination of specific IgG4 in allergy].

Measurement of the titre of specific IgG4 by the ELISA method may provide objective assistance to the control of specific immunotherapy (IS) to hymenoptera venom and grass pollens. A marked increase of the specific IgG4 titre in the first 3 months of IS or a titre already raised before the start of IS correlated with poor clinical results. Specific IgG4 can not be considered to be a diagnostic indicator in food allergy since we have frequently found this type of antibody in control subjects. Specific IgG4 in food allergy expresses a mucosal marker rather than a pathological reaction.

Adolescent↗
PubMed1987

[Determination of histamine].

Introduction of the radio-immunological technique (IMMUNOTECH, Marseille) for the measurement of histamine will in the future facilitate measurement of this mediator and this will be specially interesting during provocation tests for food and drug allergies and for histamine release in anaphylactic accidents caused by hymenoptera venom. The technique is more sensitive than that of the automated fluorimetric analysis.

Adult↗
PubMed1987

[Determination of specific serum IgG4 by an enzyme immunoassay].

Specific series IgG4 titrate is realized now with a enzymo-immunologic using "double sandwich" method with allergen and patient serum in presence of a conjugate with peroxidase monoclonal Anti IgG4. Calibration obtains with a standard curve, is realized with a monoclonal IgG4 concentration is known. The first tests are in favor of correlation between specific IgG4 specific, IgE and in another way, the increase of specific IgE and specific IgG4 in a venom immunotherapy.

Animals↗
PubMed1987

[ImmunoComb--reliability and reproducibility].

ImmunoComb is a solid phase in vitro immunological test for the measurement of serum or plasma total IgE. The technique was compared with PRIST for measurement of total IgE. The study also included reproducibility, on a test serum dilution both on a complete plate and plate sections. Statistically the conclusion is that ImmunoComb is a colourimetric test that gives the exact total IgE titre with good reproducibility. Use of a photometer greatly improves the sensitivity of assessing the results.

Humans↗
PubMed1987

[Identification of common antigens of Phleum pollen and wheat. Preliminary study].

The typing of common Phleol Pollen and wheat Antigen has been realized with these Antigens Fractions from PASTEUR Institute using FPLC PHARMACIA Chromatograph. The discover of commun Antigen has been obtained with RAST Inhibition technic. It exist common Antigen fractions between phleol and wheat, but no between wheat an phleol. The results are in favor of less allergic quality of wheat.

Allergens↗
PubMed1986

[Level of total serum IgG4 using ELISA].

We report on an indirect competitive immunoenzymatic assay (ELISA) for the determination of serum total IgG4 levels. This procedure allows accurate and sensitive measurements. Normal values vary according to age and sex, with mean normal levels of 22.3 +/- 14.6 mg/dl in adults. Serum IgG4 levels observed in atopic subjects are not significantly different from those in age and sex matched normal subjects. However, IgG4 levels are significantly increased in hay fever and honey bee sensitization. Measurement of serum total IgG4 levels clearly needs to be combined with the evaluation of specific anti-allergen antibodies of this subclass in investigations of the possible reaginic or blocking activity of IgG4 antibodies.

Adult↗