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Biomedical subjects

M G Koenig

Publications and source records attributed to M G Koenig.

At least 19 recordsLinked to original sources
PubMed1974

Clearance of Candida albicans from the bloodstream of rabbits.

Candida albicans injected intravenously into rabbits were rapidly cleared from the bloodstream. Organisms injected into a peripheral vein were predominantly cleared by the lungs; organisms injected into a mesenteric vein were largely cleared by the liver. The isolated, perfused rabbit liver avidly cleared candida organisms from buffer perfusate, but clearance was enhanced when the organisms were suspended in heated normal rabbit serum and was most complete when fresh normal rabbit serum was used as the perfusate.

Candida albicans↗
PubMed1972

Antibiotic susceptibility testing of Bacteroides.

Seventy Bacteroides strains isolated from clinical materials were tested in vitro against nine antibiotics by means of modified disc diffusion and agar dilution tests. Correlating the agar dilution susceptibility with the concentrations achievable in serum for each drug tested, we found that at least 90% of the strains were susceptible to clindamycin, chloramphenicol, carbenicillin, and lincomycin. Only 40% were susceptible to tetracycline. Except for tetracycline and chloramphenicol, the disc diffusion technique exhibited inadequate predictive value in terms of "susceptibility" and "resistance" for the Bacteroides strains tested.

Agar↗
PubMed1971

Media exchange method for demonstrating killing of L-phase variants in solid media by normal human serum.

Normal human serum has been shown to kill L-phase variants in a fluid system by the action of antibody plus complement. Previous studies, however, failed to demonstrate such killing in solid media. This failure probably resulted from inhibition of complement by medium components such as agar and NaCl. A method was developed which circumvents the problems of the anticomplementary properties of agar media and the requirement of some L-phase variants for concentrations of salt that inhibit complement. Using this method, we have demonstrated in solid media the killing by normal human serum of the L-phase variants of Staphylococcus aureus, Proteus mirabilis, Streptococcus pyogenes, S. faecalis, Pseudomonas aeruginosa, and Salmonella typhi. This method provides a relatively simple and graphic means for studying host humoral factors lethal for L-phase variants.

Antibodies, Bacterial↗
PubMed1971

Serum factors and the reticuloendothelial uptake of Staphylococcus aureus. II. Role of a zymosan-adsorbable serum opsonin.

A heat-stable factor in the serum of normal rabbits adsorbed by zymosan at 16 C in the presence of ethylenediaminetetraacetic acid has been found to enhance the uptake of an encapsulated strain of Staphylococcus aureus (Smith diffuse) by the isolated perfused rabbit liver. This opsonin does not appear to be a rate-limiting component of the hemolytic complement system, properdin, immunoglobulins G or M. It does, however, require the presence of a heat-labile cofactor(s) for expression of its activity.

Adsorption↗