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M G Longlands

Publications and source records attributed to M G Longlands.

7 recordsLinked to original sources
PubMed1982

Minimisation of salicylate interference in the Glynn and Kendal paracetamol procedure.

The Glynn and Kendal technique for plasma paracetamol assay was investigated in an attempt to eliminate its susceptibility to interference from salicylate. Modification of the nitrating reaction so as to produce milder conditions by omission of hydrochloric acid, reduction of nitrite concentration, and limiting the time of reaction to 2 minutes resulted in far less interference. A further improvement was achieved by reading at 450 nm. The proposed modification reduces the interference from a salicylate concentration of 100 mg/dl to the equivalent paracetamol concentration of 12 mg/l, compared with about 70 mg/l in the unmodified method.

Acetaminophen↗
PubMed1978

Anomalous behaviour of control sera in automated versions of the Kind and King alkaline phosphatase method.

Differences in alkaline phosphatase activity of some commerical control sera were observed when comparing manual with AutoAnalyzer versions of the Kind and King procedure. This phenomenon was found to be associated with sera boosted with exogenous alkaline phosphatase and to be owing mainly to inadequate provision of buffer in some automated methods. It is suggested that a modification to the SMA procedure may lessen the problem, but the constraints of the system necessitate a compromise. There was better precision after the modification had been introduced. Awareness of this phenomenon is of paramount importance if commerical sera are to be used in any way in the calibration of automated systems for alkaline phosphatase assay.

Alkaline Phosphatase↗