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Biomedical subjects

M G Menozzi

Publications and source records attributed to M G Menozzi.

36 records · Page 2Linked to original sources
PubMed1983

Penicillin-binding proteins in Bacteroides fragilis and their affinities for several new cephalosporins.

The penicillin-binding protein (PBP) composition in two strains of Bacteroides fragilis has been investigated using 14C-cefotaxime. This microorganism is unique among the bacteria so far investigated, which show a number of PBPs ranging from 5 to 8 in possessing only three PBPs. It lacks the PBP with molecular weight (MW) lower than 50,000 daltons, which, at least in Escherichia coli, has been demonstrated not to be essential for cell growth and division. The affinities of several cephalosporins including cefoxitin, latamoxef (moxalactam), cefoperazone and cefotaxime for the Bact. fragilis PBPs have been determined by in-vitro competition experiments. The results obtained suggest that further studies with competition experiments performed using intact, growing cells may be useful to ascertain the killing targets of beta-lactam antibiotics in bacteria.

Bacterial Proteins↗
PubMed1981

[Reliability of the bacterial identification tests and of antibiotic sensitivity tests made with automatic methods].

A "MS-2 Microbiology System" (Abbott) has been used to identify and to assess the susceptibility patterns of clinically isolated bacteria from highly compromised patients. Automatically obtained results by the analysis of 300 specimens were compared to those of traditional methods. The automated system was in good agreement with API (81%) while in 4% of the strains there were major discrepancies, and 5% of the experiments were carried out with slow growing bacteria which normally give poor results in automatic systems. Antimicrobial susceptibility testing was satisfactory in 84% of the specimens with 7% of minor discrepancies and 9% of major ones. The evaluation of MIC for slow growing organisms like Haemophilus, Diplococcus and anaerobes belonging to the Bacteroides genus, was the same using either automatic or standard procedures.

Anti-Bacterial Agents↗
PubMed1979

[Applications of gas chromatography in the diagnosis of anaerobic infections].

The isolation and identification of anaerobic bacteria by biochemical tests take long time, so it is very useful the gas-chromatography to detect the volatile fatty acids produced from their metabolism. This technique can give rapidly a presumptive diagnosis of anaerobic infection so that it is possible to start immediately a correct antibiotic therapy. In this study we have examined many samples of pus and exudate, and the results have been compared with those obtained from the culture. We have found a good correlation between the detection by gas-chromatography of isobutyric and butyric acids and the isolation in the culture of anaerobic flora, and particularly of Bacteroides fragilis when the gas-chromatography detected isobutyric, butyric and succinic acids.

Anaerobiosis↗
PubMed1976

[Bacteriuria detection by dip-slide test (author's transl)].

A new dip-inoculum method markated as Microstix for detecting bacteriuria was studied to evaluate its usefulness as a screening procedure. This test was found to be reliable when compared with the standard pour plate method. However, antimicrobial agents often present in the urine samples rendered this procedure unsuitable for indiscriminate application in large hospital laboratories. This technique might conceivably be employed in mass screening programs where urine specimens are less likely contamined by antimicrobial drugs.

Bacteriuria↗
PubMedDate not supplied

A multicentre study to evaluate the effect of sulbactam/ampicillin combination on anaerobic micro-organisms.

Ampicillin combined with the beta-lactamase inhibitor sulbactam was compared with ampicillin alone, cefoxitin and metronidazole against 569 clinical strains of anaerobic organisms. The strains included 289 species of Bacteroides, 160 strains of Clostridium and 120 strains of various species of Streptococcus/Peptostreptococus, Fusobacterium, Veillonella, Eubacterium, Bifidobacterium, Actinomyces and Propionibacterium. Sulbactam/ampicillin was as effective as cefoxitin and metronidazole against all anaerobic species tested, inhibiting more than 90% of strains at the breakpoints (16, 32 and 32 mg/l, respectively). Sulbactam/ampicillin was also significantly more active than ampicillin against strains of Bacteroides, the minimal inhibitory concentration being at least four-fold lower. In contrast, the activity of the combination did not differ from that of ampicillin alone against Fusobacterium species and Gram-positive rods and cocci.

Ampicillin↗
PubMedDate not supplied

[Yersinia enterocolitica infection in thalassemia. Report of one case (author's transl)].

The Authors describe a rare case of Yersinia Enterocolitica (Y.E.) infection in a child affected by thalassemia. The onset of the disease was that of an acute enteritis with diarrhea, fever, vomiting and abdominal pain which subsequently evolved in a picture consistent with an acute appendicitis. Laparotomy was then performed and showed a marked suppurative mesenterial lymphadenitis with mild appendicular inflammation and Y.E. infection was suspected. Culture from lymphonodes confirmed the presence of Y.E. sensitive to tobramicin and CTM. The use of these chemiotherapic agents has been followed by a rapid clinical improvement. Our recent experience could suggest some practical considerations: 1) Culture of Y.E. should be routinely performed in all children affected by acute gastroenteritis and particularly in those, above 5 years of age, in which the infection can simulate acute appendicitis. 2) Special attention should be carried out in children affected by thalassemia who can easily present more serious disease often complicated by septicemia. 3) Therapy depends on the form and severity of the disease and should be always guided by in vitro sensitivity test because of the possibility of resistence of Y.E. against the most frequently used antibiotics in septicemia.

Ampicillin↗
PubMedDate not supplied

[Further researches on antimicrobial agents involvement in urine bacteriological analysis (author's transl)].

A further investigation on about 2700 urine samples for testing the presence of antimicrobial drugs (spot-test) has been carried out. Bacteriostatic agents were present in 35% of all samples analysed, Of these, 47% were urines producing no growth on culturing, 10% had a bacterial count of 10(4) and 43% of 10(5) or more. The frequency of the spot-test positive samples was correlated to the origin from the different Hospital Departments. Gram-positive bacteria were the most represented in the samples without antimicrobial agents, while the frequency of Pseudomonas aeruginosa rised in samples with antimicrobial drugs. Under both conditions no significant variation in the antibiotic-sensitivity pattern was noted in organisms isolated from the urines. Only indole-positive Proteus appeared to be more sensitive when isolated from samples containing no antimicrobial agents.

Anti-Bacterial Agents↗