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Biomedical subjects

M G Rinaldi

Publications and source records attributed to M G Rinaldi.

At least 235 records · Page 13Linked to original sources
PubMed1986

Effect of medium composition on results of macrobroth dilution antifungal susceptibility testing of yeasts.

A total of 62 different clinical yeast isolates were examined for susceptibility to four antifungal agents, amphotericin B, 5-fluorocytosine (5-FC), ketoconazole, and miconazole, using synthetic amino acid medium-fungi (SAAM-F), buffered yeast nitrogen broth (BYNB), Kimmig broth, casein-yeast-glucose broth (CYG), antibiotic medium 3-FDA (ANTI-3), and tryptic soy broth (TSB). A macrobroth dilution format was used with MICs determined after incubation for 24 and 48 h. All analyses were performed in duplicate. In general, MICs were more reproducible after 48 h of incubation. Furthermore, with certain medium-antifungal agent combinations, MICs determined after incubation for 48 h were significantly higher than those determined after 24 h. For instance, with 5-FC irrespective of the medium used, greater than 25% of all 48-h MICs were more than one twofold dilution higher than the corresponding MICs determined after incubation for 24 h. Similar observations were made with amphotericin B when tested with BYNB and CYG and with the imidazoles when tested in all of the media except CYG. The actual MICs obtained with the different antifungal agents were clearly influenced by the test medium used. The rank order of amphotericin B MICs according to test medium was as follows: BYNB greater than SAAM-F = Kimmig = CYG = ANTI-3 = TSB. With 5-FC, the following pattern was observed: Kimmig = ANTI-3 greater than SAAM-F = CYG = TSB greater than BYNB. For both imidazoles, ketoconazole and miconazole, the rank order of MICs according to test medium was BYNB = Kimmig = CYG = ANTI-3 = TSB greater than SAAM-F. The results of this investigation suggested that broth dilution susceptibility testing of yeasts is best performed with an incubation period of 48 h. Furthermore, medium composition can significantly influence the results of such testing.

Amphotericin B↗
PubMed1985

Fungal peritonitis caused by Lecythophora mutabilis.

Fungal peritonitis caused by Lecythophora mutabilis, a mold rarely isolated from humans, is described. A patient on continuous peritoneal dialysis developed clinical, microbiological, and serological evidence for peritonitis due to this fungus. In vitro susceptibility testing of the fungus revealed marked differences in the activities of various antifungal agents. Although initially responding to treatment with oral ketoconazole, intraperitoneal miconazole, and catheter replacement, the patient had a documented relapse. The patient was eventually cured following the removal of a second catheter in association with prolonged imidazole treatment.

Antifungal Agents↗
PubMed1983

Mycetoma or pseudomycetoma? A distinctive mycosis caused by dermatophytes.

A 13-year old black girl presented with an unusual scalp lesion felt to be compatible with mycotic granuloma. She had undergone surgery two years earlier for removal of three similar scalp lesions. Material excised during surgical removal of the cyst-like lesion again revealed histopathology compatible with mycotic granuloma. Surgical material cultured out pure growth of a fungus identified as Microsporum canis Bodin. Subsequent histopathological study of the infected tissue resulted in a diagnosis of dermatophytic 'mycetoma'. The disease will be reviewed and discussed as regards its nomenclature, histopathology and salient clinical features.

Adolescent↗
PubMed1983

The microtrabecular lattice and its relationships to other organelles in prokaryotes and eukaryotes--high resolution scanning electron microscopy.

High resolution scanning electron microscopy demonstrated the microtrabecular lattice in bacteria, fungi, plant and animal cells. It is attached to ribosomes and plasma membranes generally, but to other organelles and the nuclear envelope in eukaryotes. The eukaryotic organelle surface substructure is described. Differentiation of real structure from artifacts of fixation, critical point drying and sputter-coating, is discussed.

Animals↗
PubMed1982

Use of potato flakes agar in clinical mycology.

A formulation comprised of commercially available potato flakes, glucose, and agar provides a medium which is inexpensive, is easily prepared, and is particularly useful for the identification of mold fungi encountered in the clinical mycology laboratory. The inclusion of appropriate antimicrobial agents results in a selective medium effective in the isolation and identification of medically important fungi.

Culture Media↗
PubMed1982

Gluteal abscess caused by Phialophora hoffmannii and review of the role of this organism in human mycoses.

Infections caused by members of the Phialophora hoffmannii (Beyma) Schol-Schwarz fungal aggregate (a group of related taxa, especially species of uncertain circumscription) are not reported frequently. This case report concerns the development of a gluteal abscess after multiple intramuscular injections of antimicrobial agents. Microbiological examination of the abscess material yielded a pure growth of the mold P. hoffmannii. Hyphal elements in the purulent exudate were only demonstrated when the specimen was digested by an N-acetyl cysteine-NaOH digestion-decontamination procedure or in stained paraffin block sections of the exudate. Other cases of disease caused by members of this fungal aggregate and the status of the mycology of this organism are reviewed.

Abscess↗
PubMed1978

Disseminated aspergillosis in a dog.

A dog with disseminated Aspergillus terreus infection died after a protracted course of hospitalization. Treatment with amphotericin B methyl ester was without effect. The causative organism was found in bone, myocardium, spleen, kidneys, liver, thymus, lymph nodes, and both eyes. Treatment with antimicrobials and corticosteroids prior to hospitalization may have contributed to dissemination of the fungus.

Animals↗
PubMed1977

Feline brain abscesses due to Cladosporium trichoides.

Cladosporium trichoides was isolated from brain abscesses of two cats. No obvious route of entry was found in either case. The brain was the only organ cultured. No other significant lesions were observed during necropsy. These are the first reported cases of such infection in animals.

Animals↗
PubMed1977

Cutaneous infection in man caused by Fusarium moniliforme.

Fusarium moniliforme was isolated from a pustular lesion on the hand of an otherwise healthy male. Branching hyphae were seen in pus stained by the periodic acid-Schiff method. Pustular lesions containing branching hyphae were produced in mice after subcutaneous injection of microconidia. The experimental infection in mice and the naturally occurring lesion in man healed without treatment in 1 to 2 weeks. Although production of catenulate microconida was abundant on several common laboratory media, the characteristic macroconidia of F. moniliforme were produced only on barley-pea-rice straw agar.

Adult↗
PubMed1976

Acremonium falciforme as a cause of mycetoma in California.

Three cases of mycetoma were studied in the San Francisco Bay area of northern California during the period from 1960 to 1973. In each case the causal agent was identified on the basis of the histological structure of the granules and by isolation of the etiological agent in pure culture. The fungus in all cases was found to be Acremonium falciforme. This is the first report of A. falciforme occurring as a disease agent in the United States.

Adult↗
PubMed1975

Mycotic flora of the interdigital spaces of the human foot: a preliminary investigation.

A qualitative and quantitative study of the mycotic flora of the interdigital spaces of 27 male volunteers yielded 1,291 moulds and 598 yeasts. Concurrently, a study of garden soil was conducted in order to obtain data concerning the transient-resident status of the fungi recovered from the feet. Of the 120 genera and species of fungi isolated, 51 were recovered from the volunteers, 53 from the soil, and 16 from both categories. The most commonly recovered fungi from the toewebs, in order to occurrence, were Torulopsis candida, Mycelia Sterilia, T. maris, Rhodotorula rubra, Cryptococcus albidus, and species of Aspergillus and Penicillium. Without sign of infection, Cryptococcus neoformans was isolated from 5 volunteers. Candida albicans was not recovered from any subject. Trichophyton mentagraphytes was recovered from 7 volunteers and T. rubrum from one.

Adult↗