Biomedical subjects

M G Vos-Maas

Publications and source records attributed to M G Vos-Maas.

3 recordsLinked to original sources

PubMed1982

Optimization and kinetics of in vitro stimulation of carp (Cyprinus carpio L.) leukocytes.

The optimization of a leukocyte stimulation microassay with carp (Cyprinus carpio L.) leukocytes is described. Leukocytes were isolated from the thymus, anterior kidney, spleen, mid-kidney and peripheral blood. Leukocyte cultures were stimulated with PHA-P, LPS (Escherichia coli 055: B5) PWM, ConA and PPD from Mycobacterium fortuitum. The optimum incubation temperature for leukocyte cultures differed 3.5 days for leukocyte cultures derived from lymphoid organs and 4.5 days for peripheral blood lymphocyte cultures. Leukocytes from various organ sources showed similar reactivity patterns to stimulation in vitro by different mitogens. The results of these mitogen stimulations did not present sufficient arguments in favour of compartmentation.

Animals↗

PubMed1982

Presence of antigen sensitized leukocytes in carp (Cyprinus carpio L) following bath immunization against Flexibacter columnaris.

Bath immunization of carp (Cyprinus carpio L) resulted in protection of fish at natural challenge. Stimulation of leukocytes derived from thymus, spleen, anterior kidney and mid-kidney of fish immunized with Flexibacter columnaris bacterin revealed the presence of antigen sensitized cells in all lymphoid tissues except the anterior kidney. After 28 days a response was obtained in thymus and spleen leukocyte cultures.

Animals↗

PubMed1976

A technique for intravenous injection in the red-eared turtle (Pseudemys scripta elegans).

A technique is described for exposure and injection of the jugular vein in the red-eared turtle. Satisfactory anaesthesia and muscular relaxation for this procedure was obtained by intramuscular injection of a mixture of chloral hydrate and sodium pentobarbital at a dose of 2.5 ml/kg bodyweight. Induction time was 30-45 minutes. Time for surgical procedure 30-45 minutes. Recovery time 1-8 hours.

Anesthesia↗