PubMed Health⌕ Search

Biomedical subjects

M G Wilkins

Publications and source records attributed to M G Wilkins.

5 recordsLinked to original sources
PubMed1988

The coated wire electrode glucose sensor.

Previous techniques for sensing and measuring glucose are briefly reviewed and compared. The genesis of the glucose coated wire electrode lies in liquid membrane technology, and the basic principle of the electrode is described. The electrode utilizes a quaternary ammonium salt with a sparingly soluble metallic salt of glucose, in a matrix such as poly(vinyl chloride). Fabrication procedures are detailed. Typical sensor responses are illustrated with experimental data. The sensor generally shows a voltage that decreases linearly with increasing glucose concentration over approximately 40 mg/dl to 200 mg/dl. The time response of the sensor is of the order of seconds, and the lifetime observed to date is of the order of several months. Initial studies of mechanism are discussed, using liquid NMR. Preliminary interference effect studies to date are summarized, in which common compounds such as ascorbic acid, uric acid, L-cystine, glycine, and bilirubin were used. Singly these compounds show no significant interference effects.

Barium↗
PubMed1983

Implantable glucose sensor.

Two new approaches for construction and testing of a miniature in vivo implantable glucose sensor are described. The first approach is to immobilize glucose oxidase onto a platinum wire, coated with special glass (Corning), and to cover the immobilized enzyme with a thin layer of polymer to prevent large molecules from poisoning the electrode. The second, and more successful, approach is to use the principle of coated wire electrodes by incorporating a quaternary ammonium salt with an insoluble glucose salt and poly(vinyl chloride). Potentiometric measurements were used to test the two electrodes, as well as records of voltage and current responses. Responses of the electrodes to varying glucose concentrations are described. Current work is concerned with the responses of these electrodes to plasma and blood, and in examining interference effects from blood chemicals and electrolytes. Alternative methods for preparation of the coated wire electrodes are under investigation, as is their mechanism of action.

Blood Glucose↗
PubMed1979

Effect of aspirin and vitamins C and E on synovial rheumatoid arthritic and other cells.

Normal and rheumatoid arthritic human synovial cells, normal rat muscle and bone cells, were cultured with combinations of aspirin (acetylsalicytic acid), vitamins C and E. Aspirin reduced percent growth of all cells by about 1/5 relative to controls. High vitamin C eradicated arthritic cells. In combinations, vitamin C was most important in eradicating arthritic cells. A low-vitamin C combination was most effective in reducing arthritic cell populations, while having little effect on normal cells. Vitamin E retarded but did not prevent the action of vitamin C.

Arthritis, Rheumatoid↗