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M GOLDMAN

Publications and source records attributed to M GOLDMAN.

At least 19 recordsLinked to original sources
PubMed1964

PATHWAYS OF GLUCOSE CATABOLISM IN BACILLUS CEREUS.

Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Pathways of glucose catabolism in Bacillus cereus. J. Bacteriol. 87:377-386. 1964.-Estimates by a radiorespirometric method of the pathways of glucose catabolism of resting-cell suspensions of Bacillus cereus strain terminalis indicate that the Embden-Meyerhof pathway predominates at every stage of development, including the sporogenic and germinative phases. At the filamentous, granular, forespore, and transitional stages, 98% of the glucose was catabolized by the Embden-Meyerhof pathway, and the remainder by the hexose monophosphate oxidative pathway. Estimates of the pathways in resting spore-suspensions arrested at defined stages of development indicate that 20% of the glucose was catabolized through the hexose monophosphate pathway in germinated spores, and 10% in the swollen and elongated stages of postgermination. In cells which had completed the first cell division, the figure fell to about 2%, a level similar to that found for vegetative cells at later stages of development. The key Embden-Meyerhof enzymes, hexokinase, phosphohexoisomerase, phosphofructokinase, and aldolase, as well as several other enzymes, were present at all stages of germination and postgerminative development, supporting the radioisotopic data obtained with whole cells. As indicated by the release of C(14)O(2) from glucose-6-C(14), terminal respiration of resting-cell suspensions operates maximally in vegetative cells at the granular, fore-spore, and transitional stages. There was marked inhibition of terminal respiration during the development of spores into vegetative cells. Only slight activity occurred in the earliest vegetative stages, and maximal operation developed after about ten cell divisions. Fumarase was absent in spores until sometime late in the elongation stage. At this point, a weak but definite activity appeared which increased during later stages of development so that, by the end of about the sixth cell division, fumarase had a specific activity about 80 times that observed at elongation.

Bacillus cereus↗
PubMed1964

CHANGES IN TERMINAL RESPIRATORY PATHWAYS OF INTACT CELLS OF BACILLUS CEREUS AT VARIOUS STAGES OF DEVELOPMENT.

Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Changes in terminal respiratory pathways of intact cells of Bacillus cereus at various stages of development. J. Bacteriol. 87:387-390. 1964.-The conversion of glucose-6-C(14) to C(14)O(2) was used as the indicator of terminal respiration. Resting suspensions of cells that had completed their first division exhibited little terminal respiratory activity, whereas cells that had completed eight to nine divisions exhibited maximal activity. Intermediate values were obtained for resting-cell suspensions at developmental stages between the first and eighth divisions. Growing cells at all stages oxidized considerably less glucose-6-C(14) to C(14)O(2) than did comparable resting cells. Terminal respiration was demonstrable in resting cells harvested from a growth medium still containing relatively large amounts of glucose.

Bacillus cereus↗
PubMed1963

INTERRELATIONSHIP BETWEEN TEMPERATURE AND SODIUM CHLORIDE ON GROWTH OF LACTIC ACID BACTERIA ISOLATED FROM MEAT-CURING BRINES.

Goldman, Manuel (American Meat Institute Foundation, Chicago, Ill.), R. H. Deibel, and C. F. Niven, Jr. Interrelationship between temperature and sodium chloride on growth of lactic acid bacteria isolated from meat-curing brines. J. Bacteriol. 85:1017-1021. 1963.-An elevation of the temperature limit for growth of some Pediococcus homari (Gaffkya homari) and motile Lactobacillus strains could be effected by the addition of sodium chloride to the growth medium. At the optimal temperature for growth, sodium chloride was stimulatory, and as the temperature of incubation was increased a mandatory requirement for sodium chloride was manifested. At the optimal temperature for growth (30 C), the highest sodium chloride concentrations were tolerated; as the temperature was increased, this tolerance decreased, although the optimal sodium chloride concentration increased. No other substances were found that would replace the sodium chloride requirement at higher temperatures of incubation.

Food Preservation↗