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Biomedical subjects

M Galvez

Publications and source records attributed to M Galvez.

7 recordsLinked to original sources

Factors in the accumulation of dieldrin in broiler organs: quantity ingested, sex and weight of animal.

The effect of several factors in relation to the quantities of dieldrin accumulated in different organs of broilers was studied. Feedstuff was contaminated at doses of 60, 90, 120, 200 and 240 ppm. With the doses used, the results show an accumulation summit over which the amounts accumulated are not affected by greater quantities of dieldrin ingested. The accumulation of dieldrin in the female is greater than in the male and the weight of the animal does not significantly affect the response.

Animals

Separation by counter-current distribution of rat and chicken erythrocytes of different age, and its application to the assay of enzyme activities.

The separation of cells with different ages from erythrocyte populations of adult rats and young or adult chickens have been achieved by counter-current distribution (CCD). A thin-layer CCD apparatus has been employed. Erythrocytes from blood samples taken at different times after 59Fe i.p. injection were separated by CCD. By compilation in a "composite curve" of the hemoglobin and radioactivity CCD profiles obtained for each erythrocyte population, the distribution of cells according to age can be inferred. Young erythrocytes of rats are located at the right part of the CCD curves, while older cells are distributed towards the left. An opposite distribution has been found for erythrocytes from adult or young chickens. As a first attempt for the application of the CCD procedure to the assay of enzyme activities, it was found a decrease in phytase activity as the age of chicken erythrocytes increases and an increase in phosphoglycerate kinase and phosphofructokinase as the age of rat erythrocytes increases.

6-Phytase

Study of luteal function.

On studying various parameters of luteal function, the authors demonstrated that BTT does not permit the evaluation of luteal phase defects, whereas the E.I. and prolactin levels, as well as the endometrial biopsy, may be considered valid parameters. The TRH test is more efficient than basal prolactin evaluation in this respect. Progesterone should be evaluated in various moments of the luteal phase and not by a single determination. The authors conclude that the abnormal luteal phase is the result of an abnormal follicular maturation and that the central origin of this mechanism may be affirmed.

Biopsy