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Biomedical subjects

M Gan

Publications and source records attributed to M Gan.

17 recordsLinked to original sources
PubMed2005

Chriz, a chromodomain protein specific for the interbands of Drosophila melanogaster polytene chromosomes.

Polytene interphase chromosomes are compacted into a series of bands and interbands reflecting their organization into independent chromosomal domains. In order to understand chromosomal organization, we set out to study the role of proteins that are selective for interbands. Here we describe the Drosophila melanogaster chromodomain protein Chriz that is coimmunoprecipitated with the zinc finger protein Z4. Both proteins colocalize exclusively to the interbands on Drosophila polytene chromosomes. Like Z4, Chriz is ubiquitously expressed throughout development and is associated with chromatin in all interphase nuclei. Following dissociation from chromatin, early in mitosis Chriz binds to the centrosomes and to the mitotic spindle. Newly induced amorphic Chriz alleles are early lethal, and ubiquitous overexpression of Chriz is lethal as well. Available Chriz hypomorphs which survive until pupal stage have a normal chromosomal phenotype. Reducing Z4 protein does not affect Chriz binding to polytene chromosomes and vice versa. Z4 is still chromosomally bound when Chriz protein is depleted by RNA interference.

Alleles↗
PubMed2003

Urogynaecological and obstetric issues in women with the exstrophy-epispadias complex.

OBJECTIVE: To review of the sexual and urogynaecological issues faced by a large cohort of women with the exstrophy-epispadias complex (EEC). PATIENTS AND METHODS: The study comprised 83 women and girls with EEC; a confidential survey was mailed to identify their social and sexual concerns. Fifty-six women had classical bladder exstrophy (CBE), 13 had female epispadias (FE) and 14 had cloacal exstrophy (CE). Data on the initial method of reconstruction and urogynaecological problems were obtained from a review of the hospital records. Information on continence, infection and sexual function was obtained from 34 completed surveys. RESULTS: The bladder was closed in 51 patients with CBE and 13 with CE. Urinary calculi developed in 10 patients with CBE, two with FE and three with CE. Vaginal and uterine prolapse occurred an earlier age in patients with EEC. Eight women had 13 pregnancies, eight of which resulted in normal healthy children. Overall continence was achieved in 85% of the women surveyed. Urinary tract infections remained a frequent problem for women with EEC; only 27% of respondents indicated that they were infection-free. Women aged > 18 years (24) who responded indicated that they had appropriate sexual desire; 16 were sexually active and the mean age for commencing sexual activity was 19.9 years. Six patients had dyspareunia and 10 indicated that they had orgasms. However, five additional patients indicated that they had restricted intercourse, as they were dissatisfied with the cosmesis of their external genitalia. CONCLUSIONS: Sexual and gynaecological issues become increasingly important in patients with EEC as they become adults. Understanding these issues faced by patients with EEC as they mature will permit better counselling of future patients.

Adolescent↗
PubMed2000

[The method of time series modeling and its application in the spectral analysis of lubricating oil].

In this paper, we discuss the applications of time series modeling method in the analysis of lubricating oil of mechanical equipment. We obtained satisfactory results by applying AR model to perform time series modeling and forecasting analysis to the collected spectral analysis data of the air engine. So we have built a practical method for state monitoring and trouble forecasting of mechanical equipment.

English Abstract↗
PubMed1997

[Comparison on pharmacological functions of pheretima].

Hot water extraction of Pheretima have marked actions in antipyretic, hypotensive, antiasthmatic and diuresis. Among them Guang-Pheretima and Hu-Pheretima are stronger than Tu-Pheretima in antipyretic. The difference of the intensity of Pheretima in the other actions is not obvious.

Animals↗
PubMed1995

[A study on antimicrobial resistance of coagulase negative staphylococci].

One hundred and eight strains of coagulase negative staphylococci (CNS) isolated from different clinical specimens (sputum, urine, blood, various catheters etc.) were classified into 9 species of CNS with biochemical technique. Staphylococcus epidermidis and staphylococcus saprophyticus accounted for the majority (66%). Drug susceptibility tests revealed that they were highly resistant to penicillin, oxacillin, erythromycin, chloramphenicol, compound sulfamethoxazole, tetracycline, cephazolin and ++ceftazidim but relatively sensitive to vancomycin, amikacin, gentamicin and rifapen. The MIC of oxacillin ranged from 0.125 mg/L to 128 mg/L. The MIC90 was 64 mg/L. It is suggested that vancomycin is the therapy of first choice in methicillin resistant CNS infection.

Anti-Bacterial Agents↗
PubMed1989

Quantitative iodine-123 IMP imaging of brain perfusion in schizophrenia.

Decreased perfusion in the frontal lobes of patients with chronic schizophrenia has been reported by multiple observes using a variety of techniques. Other observers have been unable to confirm this finding using similar techniques. In this study quantitative single photon emission computed tomography brain imaging was performed using p,5n [123I]IMP in five normal subjects and ten chronically medicated patients with schizophrenia. The acquisition data were preprocessed with an image dependent Metz filter and reconstructed using a ramp filtered back projection technique. The uptake in each of 50 regions of interest in each subject was normalized to the uptake in the cerebellum. There were no significant confirmed differences in the comparable ratios of normal subjects and patients with schizophrenia even at the p = 0.15 level. "Hypofrontality" was not observed.

Adult↗
PubMed1988

Detection of malignant melanoma with iodine-123 iodoamphetamine.

Iodoamphetamine (IMP) was shown by in vitro assay to have a high uptake by human melanotic melanoma cells, as compared to amelanotic melanoma cells. Eleven patients with proven malignant melanoma (MM) and 3 normal subjects were imaged at 2-4 hr and 16-24 hr after the i.v. injection 5 mCi (185 MBq) of [123I]IMP. One patient had a recurrent tumor that was subsequently shown to be squamous cell carcinoma. The index lesion was not visualized in the three patients with amelanotic melanomas. The index lesion/lesions were visualized in six of the seven other patients, except for 4/16 nodules in one patient. The seventh patient had a large, necrotic melanotic tumor that was not visualized, but an unsuspected lesion in the iliac nodes was detected. Multiple unsuspected lesions were detected in a second patient. While many lesions were seen at 2-4 hr, all lesions (other than a patient with small bowel disease) were seen best at 16-24 hr. No eye uptake was observed in any patient or control subject. Testicular uptake was seen in all males at 16-24 hr. Iodine-123 IMP appears to be a useful agent for the detection and follow-up of patients with melanotic MM.

Adult↗
PubMed1968

Improved method for the preparation of malonyl coenzyme A.

Malonyl coenzyme A is synthesized by transacylating S-malonyl-N-decanoyl cysteamine with coenzyme A. A simplified procedure for the preparation of S-malonyl-N-decanoyl cysteamine, using cysteamine as starting material, is described.

Coenzyme A↗
PubMedDate not supplied

Chinese mothers' reactions to their child's chronic illness.

Improvements in health status in the People's Republic of China have led to a shift in the burden of illness from acute, communicable disease to long-term illness, particularly in urban areas. The Chinese family's response to chronic illness is influenced by many factors, including the traditional cultural value of family responsibility as well as economic determinants. Through direct interviews with 75 women, we sought to understand the financial, social, emotional, and physical effects of caring for a chronically ill child on a Chinese mother's life and caregiving ability and to identify the sources of support available to her.

Adolescent↗
PubMedDate not supplied

The experience of the family of children with chronic illness at home in China.

The increasing prevalence of the nuclear family in China challenges the cultural ideal and tradition of the extended family caring for children with chronic illness at home. This study examined and documented the caregiving experiences in a sample of this population of children and their families. A descriptive survey with both quantitative and qualitative questions was developed and used to interview a total of 75 families in three urban areas of China. Factors such as the severity of illness, physical symptoms, the amount of assistance the child needed for daily living, and the emotional and social impact on the child were identified. Descriptions of the family's living circumstances, caregiving situations, and the child's experiences are illustrated through the use of three case studies.

Adaptation, Psychological↗