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Biomedical subjects

M Ganguly

Publications and source records attributed to M Ganguly.

30 records · Page 2Linked to original sources
PubMed1977

Positive feedback of oestrogen on LH secretion in women in neuroleptic drugs.

The effect of a-adrenergic and dopaminergic blockade on the ability of pituitary-hypothalamic axis to produce an LH surge in response to oestrogen, was studied in eleven women treated with the neuroleptic agents haloperidol, fluphenazine or chlorpromazine. Seven women on no medication were controls. 6.6 mg of oestradiol benzoate (OB) was injected i.m. after control serum was obtained and daily serum samples were obtained for four additional days. Sera were assayed for total oestrogens, LH, FSH and prolactin. Six of seven women in the control group and eight of eleven women treated with neuroleptic agents had LH surges to OB challenge. The mean prolactin of the treated women was 33.1 +/- 3.6(SEM) ng/ml and the mean prolactin of the controls was 14.9 +/- 1.5(SEM) ng/ml, P less than 0.005). Elevated levels of circulating prolactin did not prevent an LH surge to OB challenge. These results indicate that even in subjects with catecholamine blockade, an LH surge can be induced. This contrasts to results obtained in rats and suggests that the control mechanism of LH surge production differs in rodents and humans.

Adolescent↗
PubMed1977

Induction of an LH surge with estradiol benzoate in a patient with incomplete testicular feminization syndrome.

A 31-year-old patient with incomplete testicular feminization syndrome characterized by a 46, XY karyotype in a phenotypic female with absent müllerian structures, marked clitoromegaly, testes and wolffian duct structures, partial labioscrotal fold fusion, and male levels of circulating androgens is described. She was challenged with estradiol benzoate (EB) to simulate the preovulatory surge of estradiol seen in cycling women. This EB challenge resulted in a negative feedback response for FSH but an LH surge similar to that induced with EB in cycling women. This suggests that in the incomplete testicular feminization syndrome there is hypothalamic or pituitary resistance to the blocking effects of androgens on LH surge production.

Adult↗
PubMed1976

Estrogen biosynthesis and 1beta-hydroxylation using C19 and 19-nor steroid precursors.

(1) In order to study the relationship between aromatization (estrogen biosynthesis) and 1beta-hydroxylation, the effects of a variety of factors on these processes were evaluated. (2) Using the C18 substrate, 4-estrene-3,17-dione, it was found that carbon monoxide, SU-4885, amphenone B, potassium cyanide, 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione inhibited the above transformations significantly and to varying degrees. However, within a given experiment the inhibition of each process was similar. (3) SKF-525A did not inhibit either transformation. In addition, phosphate, Tris and barbital buffers, as well as pH changes from 6.9 to 7.7, had no stimulatory or inhibitory effect on the production of estrogen and 1beta-hydroxy compounds. (4) In contrast, several inhibitors affected the aromatization of C19 and C18 steroids differently. These include carbon monoxide, SU-4885 and amphenone B. (5) When a mixture of 4-[7beta-3Hi1estrene-3,17-dione and 19-[4-14C]nortestosterone were incubated together the former was preferentially converted to estrogen. This preference for the 17-keto steroidal form mimics results observed for C19 substrates. (6) We conclude that while estrogen biosynthesis and 1beta-hydroxylation appear to be mediated by the same enzyme system, the same conclusion cannot be drawn for the aromatization of C19 and C18 substrates.

Buffers↗
PubMed1976

Induction of an LH surge with estradiol benzoate. A clinical test of pituitary-hypothalamic axis competence.

Intramuscular injection of 6.6 mg of estradiol benzoate (EB) in oil was used in 34 women as a test of the ability of the pituitary-hypothalamic axis to produce a gonadotropin surge (positive-feedback response). These responses were compared to Clomid responses. All of the normal cycling women, two-thirds of oligo-ovulatory but menstruating women, and one-third of amenorrheic women had LH surges to EB challenge. Estradiol benzoate is a more specific test of positive feedback response than Clomid. It also may be effective in inducing ovulation in some amenorrheic patients refractory to Clomid treatment.

Adult↗
PubMed1975

Serum unconjugated estriol in the menstrual cycle and early pregnancy.

A radioimmunoassay for serum unconjugated estriol in the menstrual cycle with a sensitivity of about 5 pg/ml is described. 8 cycles were studied. In 2 cycles, single spikes of 22 and 30 pg/ml were obtained. In 3 cycles, concentrations of 4-5 pg/ml were found whereas in the other 3 studies, no estriol was detected. In general, peaks of estriol corresponded to peaks in estradiol plus estrone. Patients in 4-12 weeks of gestation were also studied. Concentrations as high as 262 pg/ml were found but in isolated instances, no estriol was detected. The results support the view that in contrast to the pregnant state, in the normal menstrual cycle, the bulk of the estriol produced is conjugated before release into the blood.

Estriol↗