Biomedical subjects

M García-Caballero

Publications and source records attributed to M García-Caballero.

8 recordsLinked to original sources

PubMed1992

Simultaneous fluorometric determination of intracellular polyamines separated by reversed-phase high-performance liquid chromatography.

A reversed-phase HPLC technique in combination with fluorescent detection is described for simultaneous quantification of the precolumn Dansyl derivatives of intracellular amines. The derivatives were stable for at least one week, kept protected from the light at -20 degrees C. The detection limit was between 1 and 5 pmol for all tested polyamines. Serotonin coeluted with tryptamine. The method has a very good reproducibility for both, retention times and chromatographic peak areas. The average recovery of standard amine solutions added to cellular extracts was estimated to be higher than 90%. The described method enables a rapid, reliable and reproducible quantification of biogenic and related polyamines in biological fluids and tissues.

Animals↗

PubMed1990

Evaluation of key gluconeogenic enzymes in experimental biliary obstruction.

In order to evaluate the usefulness of key gluconeogenic enzymes, in relation to the markers commonly used (alkaline phosphatase and gamma-glutamyl transpeptidase) for the diagnose of cholestasis the serum activity of phosphoenolpyruvate carboxykinase, fructose 1,6-bisphosphatase and glucose-6-phosphatase has been measured in rats with bile-duct ligation. Among the gluconeogenic enzymes studied only phosphoenolpyruvate carboxykinase activity increased significantly in the first 48 hours after cholestasis, decreasing thereafter to normal values. Both alkaline phosphatase and gamma-glutamyl transpeptidase activities showed a very significant increase which persisted throughout the experiment. These results seem to indicate that in spite of the high organ specificity of these enzymes they do not appear to be useful for the diagnosis of cholestasis.

Alanine Transaminase↗

PubMed1989

Histamine and serotonin inhibit induction of ornithine decarboxylase by ornithine in perifused Ehrlich ascites tumour cells.

Ornithine induced more than 36-fold the ornithine decarboxylase activity in confined Ehrlich ascites tumour cells after 3.5 h of continuous perifusion with 0.5 mM ornithine; arginine and glutamine also induced the activity 3- and 4-fold, respectively. The addition of cycloheximide or actinomycin D antibiotics to the perifusion medium confirmed that the regulation of the enzyme synthesis takes place at the level of translation. Perifusion in the presence of 0.5 mM ornithine and 55, 25, and 10 microM histamine suppressed the induction by 91, 53, and 35% respectively. Similar results were obtained in the presence of serotonin. Histidine also showed inhibitory effect but 5 mM histidine was required to produce 21% inhibition; other basic amino acids were ineffective.

Amino Acids↗

PubMedDate not supplied

The evolution of postoperative ileus after laparoscopic cholecystectomy. A comparative study with conventional cholecystectomy and sympathetic blockade treatment.

Our study is prompted by the arrival of laparoscopic cholecystectomy in connection with the evolution of postoperative ileus (PI) and by its avoidance of the intraabdominal handling implied in conventional cholecystectomy. With this aim a prospective, controlled, randomized, and blind clinical trial was designed using 100 patients divided into five groups (n = 20): I, conventional cholecystectomy (CC): II, CC+injection of 20 ml bupivacaine 0.5% into the mesentery root; III, CC + 7.5 mg propranolol i.v. and 0.5 mg neostigmine s.c., postoperatively until the first defecation; IV, II+III; and V, laparoscopic cholecystectomy. The shortest period of PI was observed in group V. This period increases notably in group IV (53 h), group II (72 h), and group III (84 h) relative to the control group with (89 h). This reduction in PI time runs parallel with an improvement in the patient's general state of well-being. We concluded that after laparoscopic cholecystectomy PI is nonexistent. Furthermore, this study confirms the correlation between the avoidance of intraabdominal manipulation and the evolution of postoperative ileus.

Autonomic Nerve Block↗

PubMedDate not supplied

[Dysautonomic seizures in patients admitted to an intensive care unit following severe traumatic brain injury].

AIM: To describe clinical and radiologic features of dysautonomic crisis after severe traumatic brain injury and its influence in the clinical situation six months later. PATIENTS AND METHODS: Retrospective, observatory study of seven patients after severe head injury, with dysautonomic crisis, admitted in the Critical Care Unit (CCU) during six months. No interventions. Its clinical features, its association with intracranial pressure and the treatment for Dysautonomic crisis they have received were extracted from the computed clinical report. We have evaluated his neurologic prognosis with the Jennet-Bond scale and his radiologic characteristics with the Gennarelly scale. RESULTS: Dysautonomic crisis began in the first week if the patients didn't received neuromuscular blocks drugs and they continued when the patients were discharged from the CCU in a young population with an initial Glasgow scale coma of 5 points. We didn't wait a special radiologic pattern. After the next six months, crisis were disappeared in 86% of patients and all patients reached a good neurologic level in the Jennet-Bond scale. CONCLUSIONS: Dysautonomic crisis appeared early in young men after severe head injury. We didn't find a radiologic pattern that predisposes the dysautonomic crisis. They didn't interfere the recovering six months after head injury.

Adolescent↗

PubMedDate not supplied

One anastomosis gastric bypass: a simple, safe and efficient surgical procedure for treating morbid obesity.

The One Anastomosis Gastric Bypass has been developed from the Mini Gastric Bypass procedure as originally described by Robert Rutledge. The modification of the original procedure consists of making a latero-lateral gastro-jejunal anastomosis instead of a termino-lateral anastomosis, as is carried out as described in the original procedure. The rationale for these changes is to try to reduce exposure of the gastric mucosa to biliopancreatic secretions because of their potentially carcinogenic effects with longer term exposure, which is the major criticism of the original technique. If we fix the jejunal loop to the gastric pouch some centimetres up to the gastro-jejunal anastomosis the biliopancreatic secretions have less possibility of coming into the gastric cavity (gravity force). Furthermore, if the anastomosis is latero-lateral this possibility is reduced even more. In addition, the intestinal loop reinforces the staple line against disruption, and also the gastric pouch against dilatation.

Anastomosis, Surgical↗

PubMedDate not supplied

Surgery to modify nutritional behaviour.

The main aim of obesity surgery is to change the dietary habits of morbidly obese patients. These are patients whose dietary habits nutritionists and psychologists have not been able to change during previous decades. The history of this surgery can teach us many important lessons. For example, procedures that have focused on effecting a malabsorptive state, but without addressing any behavioural mechanism for weight control, such as jejunoileal bypass, have failed. On the other hand, those that have centered on only addressing behavioural issues, the purely restrictive, are also difficult for patients to comply with and also have a high failure rate. To facilitate a change in the nutritional behaviour of morbidly obese patients which can lead to the loss of an adequate amount of weight, and which could be maintained in the long term is difficult. We need to stimulate changes that can be easily followed by the patient, and at the same time, provoke minimal medium and long term alterations in their nutritional state. To achieve and maintain this aim efficiently, it is necessary that the patients have confidence in and respect the physician, so that they can follow strictly their medical advice.

Bariatrics↗

PubMedDate not supplied

Middle term intestinal adaptation after massive distal small bowel resection in oral feeding dogs.

Intestinal adaptation (IA) consists mainly in a increase of the normal cell proliferative rate in the crypts of the intestinal mucosa. Histidine- and ornithine-decarboxylase (HDC and ODC, respectively) are always involved in the rapid growing tissue process. The present experiment studies the relation of HDC and ODC, the morpho-functional changes of the jejunal mucosa remnant and the nutritive state 2, 3 and 4 weeks after performing a 75% distal small bowel resection (DMSBR) in dogs fed with a standard chow. Each animal was its own control measure in a healthy state measured before provoking the DMSBR. The results demonstrated that 14 days after DMSBR, the mucosa of the jejunal remnant showed a loose of the normal characteristics of the villi and enterocyte morphology ("mucosal microinjuries"), accompanied by an increase in the depth of the crypt and in the HDC and ODC levels, while the D-glucose and L-phenylalanine absorptive capacity did not vary compared with that of the mucosa of the same animals in a healthy state. This correspond with a statistically significant altered nutritive state parameters. However, 21 days after DMSBR, the intestinal remnant mucosa initiates a structural recovery process. It was also observed a significant increase in the HDC and ODC enzymatic levels accompanied by an increased absorptive capacity and an improvement in the nutritive state parameters. Twenty eight days after DMSBR, the findings revealed a similar trend. In conclusion, in our model, between the second and fourth week the IA process is accompanied by a progressively increased HDC and ODC activities and an improvement of the nutritive state. The significance of the "mucosal microinjuries" described needs further investigation.

Adaptation, Physiological↗