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M Georges

Publications and source records attributed to M Georges.

81 records · Page 5Linked to original sources
PubMed1987

A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA.

The term "DNA fingerprint" has been used to describe the extensive restriction fragment length polymorphism associated with hypervariable minisatellites present in the human genome. Until now, it was necessary to hybridize Southern blots to specific probes cloned from human genomic DNA in order to obtain individual-specific restriction patterns. The present study describes the surprising finding that the insert-free, wild-type M13 bacteriophage detects hypervariable minisatellites in human and in animal DNA, provided no competitor DNA is used during hybridization. The effective sequence in M13 was traced to two clusters of 15-base pair repeats within the protein III gene of the bacteriophage. This unexpected use of M13 renders the DNA fingerprinting technology more readily available to molecular biology laboratories.

Animals↗
PubMed1987

Genetic variation of the bovine thyroglobulin gene studied at the DNA level.

The bovine thyroglobulin gene has been analysed for variation using restriction endonucleases. Six independent restriction fragment length polymorphisms have been identified. One of these results most probably from a 2.5-kb deletion, the others being compatible with point mutations. We determined that an individual taken at random within the Belgian White and Blue breed is, on average, heterozygous for one out of 1700 nucleotides within the thyroglobulin gene.

Animals↗
PubMedDate not supplied

Towards marker assisted selection in livestock.

In recent years, genomic tools have become available for most livestock species and are now being used routinely to map quantitative trait loci underlying the genetic variance for numerous economically important traits. Fine-mapping methods are being devised to refine the initially coarse map positions of the quantitative trait loci to the point required for marker assisted selection and, eventually, the positional cloning of the underlying genes. Mapping information on QTL is beginning to be used to increase genetic response by enhancing genetic variance, selection accuracy, selection intensity and by reducing the generation interval. Optimal use of MAS will require the development of more robust methods for the routine genotyping of preimplantation embryos for multiple markers.

Animals↗
PubMedDate not supplied

The effect of mixed selected and unselected samples on the power of QTL mapping.

It is known from previous work that selection by truncation can lead to substantial loss of power to detect linkage with a QTL We examine the effect of mixed selected and unselected samples. We show that even if a modest proportion of the sample originates from an unselected population, this loss of power is quite effectively neutralized. Moreover, we reach the unexpected conclusion that if the selection intensity in the selected subpopulation is high, the power to detect QTL may actually increase.

Animals↗