Biomedical subjects
Publications and source records attributed to M Ghemati.
Anaerobic bacteria need, for its multiplication, a reduce middle devoid of oxygen, who contains growth factors. We have prepared the prereduce middle TGY-V according to the recommendations of M. SEBALD [1]. This middle has been tested for its ability to permit the growth of the most exigent anaerobic bacteria. It could be used as a middle of transport and culture for any monomicrobial sampling that can contain anaerobic bacteria.
The antimicrobial resistance evolution of the Bacteroïdes fragilis group is the subject of international survey. Eighty-six clinical isolates collected in anaerobic service of I.P. A were tested for susceptibility to twelve antimicrobial agents. Chloramphenicol, metronidazole and imipenem proved to be the most active agents. After these agents, amoxicillin-clavulanic acid, carbenicillin and piperacillin were the most effective agents tested with respectively 15%, 17% and 17% of resistant isolates. Clindamicin and cefotaxin were active from only 70% and 65% of clinical isolates, and 71% of them were found resistant to cefotaxin with minimal inhibitory concentration above 32 ug/ml. beta Lactamasic profile determination according to Rolfe and Finegold modified method allowed to show five different beta lactamase types. The isoelectric points (pI) vary between 4.3 and 7.5 according to the enzymatic extracts of the clinical isolates. No transfer and no plasmid were observed with respectively imipenem and metronidazole resistant isolates. But, transconjugants were obtained with TRCcR isolate.
The efficacy of inhibitors (PEA, Ac Nal, Az de Na) in the isolation of strict anaerobes in polymicrobial sample. The efficacy of three inhibitors to select strict anaerobic bacteria in the polymicrobial sample had been studied. First step: The most frequent anaerobes encountered in the infections are isolated in the agar Columbia containing the different inhibitors. This step allowed us to check the inhibition of the germ we have to isolate. Next step: polymicrobial mixtures were made. The composition of which is very similar to the samples we receive in the laboratory. The swarming Proteus is the facultative anaerobic germ which gives us difficulties when isolating strict anaerobic bacteria. Then, the different mixtures were isolated separately in the agar in which the inhibitors were added. The plates containing Azide of Na and PEA gave us the best results.