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Biomedical subjects

M Gillner

Publications and source records attributed to M Gillner.

25 records · Page 2Linked to original sources
PubMed1982

High-affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin in cell nuclei from rat liver.

The intranuclear binding of radioactive 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat liver has been studied both in vivo and in vitro. Following the intravenous administration of [1,6-3H]TCDD, a maximum uptake by cell nuclei could be observed at 2 h after injection with a concurrent decrease in the cytosolic uptake. Using linear sucrose density gradient centrifugation, dextran-coated charcoal adsorption assay, DEAE-Sepharose ion-exchange chromatography, competition, enzymatic and saturation studies, a high-affinity binding protein for TCDD in liver cell nuclei could be demonstrated both in vivo and after an exchange in vitro of intravenously administered unlabelled 2,3,7,8- tetrachlorodibenzofuran (TCDBF) for [3H]TCDD. Sucrose density gradient analysis showed a size of 4-5 S for both the cytosolic and nuclear TCDD binding entity. The specific binding of [3H]TCDD to nuclear components was heat labile and saturable and had an equilibrium dissociation constant of 1.05 nM. Based on a differential susceptibility to specific hydrolases, i.e. DNAase, RNAase, trypsin and pronase, the binding entity appears to be a 4-5 S salt-extractable protein.

Animals↗
PubMed1982

The TCDD receptor in rat intestinal mucosa and its possible dietary ligands.

Induction of aryl hydrocarbon hydroxylase (AHH) by polycyclic aromatic hydrocarbons and other inducers such as 2,3,8-tetrachlorodibenzo-p-dioxin (TCDD) is known to occur following binding of the inducer to a soluble receptor protein similar to steroid hormone receptors. This receptor is usually called the TCDD receptor, since TCDD has the highest affinity of all known ligands for the receptor. In the present paper a receptor for TCDD in cytosol from rat intestinal mucosa has been studied, using isoelectric focusing in polyacrylamide gel. This receptor's biochemical properties were found to be similar to those of the TCDD-receptor in rat liver cytosol. The dissociation constant (Kd) of the 3H-TCDD-receptor complex in rat intestinal mucosa was 0.7-3.1 nM, and it was present at a concentration of 70-80 fmol/mg protein. Starvation did not significantly increase the receptor level. The affinities of some potential dietary ligands for the TCDD receptor in rat intestinal mucosa were also studied. Indole-3-carbinol had 1/2,600 of the affinity of TCDD for the receptor protein. Butylated hydroxyanisole (BHA), transstilbene oxide and quercetinpentamethylether competed even more weakly with 3H-TCDD for binding to the receptor. The biological significance of the occurrence of low-affinity ligands of dietary origin for the TCDD receptor is uncertain at the present time.

Animals↗
PubMed1982

[Clinical contribution and cerebrospinal fluid study in reticulo-histiocytic meningoencephalitis].

A case of reticulo-histiocytic meningoencephalitis is reported. Changes in the fluid cell pictures, especially giant cells and mitoses are described. Obviously, reticulo-histiocytic encephalitis and neoplastic reticulosis are different stages of development and malignity of a uniform process, the etiology of which has not been understood until now but in which an immunological reaction in the sense of a delayed type of hypersensitivity plays a considerable part.

Brain↗
PubMed1980

[The social-psychiatric night ward of the nerve clinic of the University of Halle].

The conception of social-psychiatric night-hospital will be showed in a balanced system of psychiatric preventing and rehabilitating measures in the industrial centre of Halle. The care based on a physician-patient contract and will be objectivated by documentation of behavior and psychologic tests. First results confirm expectations and necessity of this special care.

Community Psychiatry↗
PubMed1980

[Treatment of an alcoholic pre-delirium syndrome with chlormethiazol, methophenazin and chlordiazepoxide].

The equal legal of prescription of Chlormethiazol and a combination of Methophenazin and Chlordiazepoxid is demonstrated by a catamnestic examination of 52 patients with predelirant syndroms. In this way it will be shown a practicable way of an efficiently used of medicine treatment by patients with a predelirant syndrom in the out-patient department of practitioners and nerve specialists.

Adult↗
PubMed1977

[Malignant tumours of the female sex organs in the autopsy material (author's transl)].

1209 malignant tumours of the female genital organs were revealed by post-mortem examinations at the Pathological Institute of the Martin-Luther-University, Halle (GDR), in the years 1961 to 1970. They constitute 28.6 per cent of a total of 4232 malignomas including leukemias and other systemic lymphoreticular diseases that were found in females of all age groups during this period. The genital tumours were analysed concerning the localization, age distribution, size, histological classification, invasion of adjacent organs, frequency of metastases, and recurrencies, 729 (= 60.3%) of the tumours originated from the uterus, 399 (= 33.0%) from the ovaries. 95.2 per cent of all tumours were carcinomas and 65.9 per cent developed metastases. The highest frequency of metastases has been found in ovarian tumours (= 89.5%). This was mainly due to the frequency of peritoneal carcinomatosis in patients suffering from these malignomas. It is remarkable that 19.5 per cent of the genital tumours were misdiagnosed by the clinicians.

Adolescent↗