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Biomedical subjects

M Gougerot

Publications and source records attributed to M Gougerot.

5 recordsLinked to original sources
PubMed1980

99mTc labelled human leukocytes. An in vitro functional study.

A new method of human leukocyte labelling is described. 20 ml of human blood are allowed to sediment in a dextran solution and the remaining red blood cells are removed using NH4C1. The leukocyte-rich suspension is incubated with a Sn-pyrophosphate solution, and then labelled, using 20 mCi 99mTc pertechnetate. Labelling efficiency is about 30%. Functional activity was shown to be satisfactory: (1) appearance under electron microscopy was quite normal; (2) Klebsiella ingestion, zymosan-stimulated oxygen consumption and iodination were similar in labelled and unlabelled cells, and (3) chemotaxis revealed no alteration in cell migration.

Cell Separation↗
PubMed1980

Human deficiency of the sixth component of complement in a patient with meningococcal meningitis and no haemostasis abnormality.

A case of human complete C6 deficiency is reported. The patient, a 31 year old white male, was seen on the occasion of an isolated episode of meningococcal meningitis. Serum complement hemolytic and bactericidal activities were lacking and could be restored to normal by addition of appropriate amounts of purified C6. No hemostatic abnormalities were observed.

Adult↗
PubMed1980

"In vivo" kinetics of 99m-technetium labelled leucocytes in dogs and the effects of an abscess.

A new labelling method of blood white cells using "cold" pyrophosphate and 99m-technetium pertechnetate was assessed in 6 normal dogs and 6 dogs with turpentine induced abscesses (8). In normal dogs, blood kinetic studies showed a two component exponential disappearance pattern of the radioactivity, the late half-life being from 4 to 20 hours. Direct organ counting and external computer assisted gamma camera counting showed an immediate accumulation in the lungs followed by a progressive decrease. There is a marked uptake into the liver and spleen. In bearing abscesses dogs, there is an obvious increase in local radioactivity, in spite of a decreased local blood supply, allowing scintigraphic demonstration of such foci in a limb. However, the abscess model is far from perfect and further studies are required before there is a routine use in man for the detection of abscesses.

Abscess↗
PubMed1976

Pseudomonas aeruginosa endocarditis. A report of nine cases.

9 cases of Pseudomonas aeruginosa endocarditis are reported and the results of this study are compared with the data of the literature. The source of infection was known in 8 patients: 7 were nosocomial infections (cardiac catheterization in 5 cases, cardiac surgery in 2 cases). The diagnosis was made in 8 patients with left-sided endocarditis. In 1 patient tricuspid endocarditis was diagnosed on postmortem examination. Carbenicillin associated with an aminoglycoside antibiotic appeared to be the most effective treatment when prescribed for several weeks. 6 of 9 patients died of uncontrolled septicemia, 3 of whom underwent surgery which was twice performed because of poor hemodynamic status. In the other 3 patients drug administration was effective at first. However, a relapse occurred in these three cases compelling another effective antibiotic therapy. Surgery was peformed in these three patients. Valve cultures were negative in two cases and positive in 1. These 3 patients survived. They are still alive after a follow-up period of 2 or 3 years.

Adult↗