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M Gould

Publications and source records attributed to M Gould.

11 recordsLinked to original sources
PubMed1990

The internal consistency and concurrent validity of a Spanish translation of the Child Behavior Checklist.

The Child Behavior Checklist's applicability to a Hispanic sample was assessed by an examination of the instrument's internal consistency and concurrent validity. The CBCL and TRF were administered to a community sample representative of children of Puerto Rico aged 4 to 16. Cronbach's alpha was used to assess the internal consistency of empirically derived scales. The relation of CBCL and TRF scores to clinical diagnosis, adaptive functioning, and need for services served as indicators of the concurrent validity of the instrument's Spanish version. The results indicate that the total behavior problem scores on the instruments are good continuous measures of maladjustment for children in Puerto Rico. A child with high values on the scales has a high probability of being classified as a case by a psychiatrist. High levels of internal consistency were found in most subscales. Only scales comprising low prevalence problems showed poor internal consistency.

Adolescent↗
PubMed1989

Elevated intracellular Ca2+ acts through protein kinase C to regulate rabbit ileal NaCl absorption. Evidence for sequential control by Ca2+/calmodulin and protein kinase C.

Calcium/calmodulin is involved in the regulation of basal rabbit ileal active Na and Cl absorption, but the mechanism by which elevated intracellular Ca2+ affects Na and Cl transport is unknown. To investigate the roles of the Ca2+/calmodulin and protein kinase C systems in ileal NaCl transport, two drugs, the isoquinolenesulfonamide, H-7, and the naphthalenesulfonamide, W13, were used in concentrations that conferred specificity in the antagonism of protein kinase C (60 microM H-7) and Ca2+/calmodulin (45 microM W13), respectively, as determined using phosphorylation assays in ileal villus cells. W13 but not H-7 stimulated basal active NaCl absorption. H-7 inhibited changes in Na and Cl absorption caused by maximal concentrations of Ca2+ ionophore A23187 and carbachol and serotonin, secretagogues that act by increasing cytosol Ca2+, while W13 had no effect. In contrast, neither H-7 nor W13 altered the change in NaCl transport caused by the cyclic nucleotides 8-Br-cAMP and 8-Br-cGMP. These data suggest that: (a) basal rabbit ileal NaCl absorption is regulated by the Ca2+/calmodulin complex and not by protein kinase C; (b) the effect of elevating intracellular Ca2+ to decrease NaCl absorption is mediated via protein kinase C but not by Ca2+/calmodulin; (c) the effects of protein kinase C are not overlapping or synergistic with those of Ca2+/calmodulin on either basal absorption or on the effects of increased Ca2+; and (d) neither Ca2+/calmodulin nor protein kinase C are involved in the effects of cAMP and cGMP on ileal active NaCl transport.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗
PubMed1989

Testing for a pulse in seasonal event data.

Most tests for seasonal clustering are sensitive to the peak in incidence in some season (90-day interval) followed by a trough 6 months later. We present two statistics sensitive to a relatively sharp increase in disease incidence for a season superimposed on a constant incidence over the entire year: the ratchet scan, based on the maximum number of events in k consecutive months, and the continuous circular scan, the maximum number of events in d consecutive days. We tabulate the tail distribution of the ratchet scan for N less than or equal to 35 and k = 2 and 3, and find the asymptotic distribution which is applicable for N greater than or equal to 50 when k = 2 or 3. We evaluate an approximation for the distribution of the continuous scan. The statistics are applied to investigate seasonal clustering of adolescent suicide in the United States. An illustration is given of the application of the circular scan, and a small simulation study and the example are used to compare the statistics with others proposed in the literature.

Adolescent↗
PubMed1987

Electrical responses of eggs to acrosomal protein similar to those induced by sperm.

The earliest known response of eggs to sperm in many species is a change in egg membrane potential. However, for no species is it known what components of the sperm cause the opening of the egg plasma membrane channels. Protein isolated from sperm acrosomal granules of the marine worm Urechis caused electrical responses in oocytes with the same form, amplitude, and ion dependence as the fertilization potentials induced by living sperm. Sperm initiated fertilization potentials in oocytes when sperm-oocyte fusion, but not binding, was inhibited by clamping oocyte membrane potentials to positive values. Acrosomal protein also initiated electrical responses in clamped oocytes. These results support the hypothesis that it is the sperm acrosomal protein that opens ion channels in the oocyte membrane.

Acrosome↗
PubMed1986

Advantages of picrate fixation for staining polypeptides in polyacrylamide gels.

When acetic acid-urea polyacrylamide gels with or without Triton X-100 were immersed in 0.1 M Na picrate, pH 7, to which 1/4 vol Coomassie blue staining solution (0.2% in 45% methanol, 10% acetic acid, 45% water) was added, proteins stained rapidly (within a few minutes in gels without Triton and within an hour in gels with Triton) with little or no background staining. Thus protein bands could be observed in a single step with no destaining. The picrate-Coomassie blue method fixed and stained a small peptide (bradykinin, nine amino acids) that was not observed in gels stained with fast green, silver, or Coomassie blue following fixation in 50% trichloroacetic acid. The picrate-Coomassie blue method gave high-contrast bands suitable for densitometry. Gels containing sodium dodecyl sulfate were also stained by the picrate-Coomassie blue method if they were first washed briefly (1 h) in 45% methanol, 10% acetic acid, 45% water, presumably to remove the detergent. These gels also stained rapidly with almost no background.

Acrylic Resins↗
PubMed1986

Fear of death and dying in medical students: effects of clinical experience.

Changes in the perception of death and dying during medical training were assessed, to test the hypothesis that fear is increased by exposure to dying patients. In general, clinically experienced medical students did not consistently differ from clinically inexperienced students in the intensity of fear of death. The two groups did differ in the frequency with which they thought about death. Students with clinical experience reported thinking more frequently about their own dying process and about interacting with a dying patient.

Adult↗
PubMed1986

Computed tomography of abdominal carcinoid tumour.

Ten patients with pathologically proven abdominal carcinoid tumour were assessed by computed tomography (CT). Post-mortem examination correlation was obtained in two cases. Computed tomography demonstrated the extent of intra-abdominal tumour well and is, therefore, a useful staging technique for patients being treated with adjuvant therapy. The appearances of metastatic carcinoid within the mesentery on CT are characteristic and can enable a pre-operative diagnosis to be made.

Abdominal Neoplasms↗