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Biomedical subjects

M Grand

Publications and source records attributed to M Grand.

At least 19 recordsLinked to original sources
PubMed2003

Comparison of media for enumeration of Clostridium perfringens from foods.

Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens (SFP) agar, sulfite cycloserine azide (SCA), differential clostridial agar (DCA), and oleandomycin polymyxin sulfadiazine perfringens (OPSP) agar] were compared in a prestudy, of which four (IS, TSC, SCA, and DCA) were selected for an international collaborative trial. Recovery of 15 pure strains was tested in the prestudy and recovery of one strain from foodstuffs was tested in the collaborative trial. Results from the prestudy did reveal statistical difference of the media but recoveries on all media were within the microbiological limits (+/-30%) of IS, which was set as a reference medium. Recoveries on the media tested in the collaborative trial were statistically different as well, but these differences were of no microbiological-analytical relevance. Food matrices did not affect the recovery of C. perfringens in general. DCA and SCA, in particular, are labor-intensive to prepare and DCA frequently failed to produce black colonies; gray colonies were quite common. Since IS medium is nonselective, it was concluded that TSC was the most favorable medium for the enumeration of C. perfringens from foods.

Animals↗
PubMed1989

[Hypothenar aneurysm of the ulnar artery. Apropos of a case treated by excision and anastomosis].

A case of aneurysm of the ulnar artery, located in the region of the hypothenar eminence is reported. This true traumatic aneurysm had a reconstructive treatment, considering the arterial anatomy of the patient's hand. A resection followed by an end to end anastomosis has been done with a good post-operative result at two years and a half. The unusualness of this pathology, the predominance of its traumatic and occupational etiology are emphasized. True and false traumatic aneurysms are described. The diagnosis of aneurysm is primarily clinical. Arteriography is interesting in the preoperative check-up. Reconstructive surgery is now preferred.

Anastomosis, Surgical↗
PubMed1986

Inorganic pyrophosphatase activity in a plaque calcifying microorganism: Bacterionema matruchotii.

The presence and activity of an alcaline pyrophosphatase of Bacterionema matruchotii, a Gram positive filamentous organism present in the bacterial plaque and calculus was studied. A strong enzymatic activity was demonstrated. The optimal conditions for this activity (alcaline pH, magnesium cofactor) have to be related to the pyrophosphatase conditions found in other calcifiable structures. The activity was shown in intact bacteria as well as in the culture medium devoid of micro-organisms. The strongest activity having been demonstrated in broken cells, it can be postulated that the enzyme is active inside the cell. The hypothesis of a contribution of Bacterionema matruchotii to the mineral phase formation is proposed; it is possible that the pyrophosphatase produced by this microorganism contributes to the mineralization process involved in dental calculus formation.

Actinomycetaceae↗
PubMed1984

[Hepatitis B virus markers, beta 2 microglobulin and anti HTLV in a population of blood donors from a prison environment].

212 male prisoners were collected at the prison in March 1983. Anti-HBc and HBs Ag were detected by a combined test (AUSRIA-CORE, Abbott Laboratories). 67 sera (31,5%) were anti-HBc positive, 7 of them HBs Ag positive. Screening for anti-HTLV (anti-p24) was negative for all the donors. Beta 2 microglobulin levels were determined on 115 sera (B2-micro RIA 100, Pharmacia Laboratories). 8 had levels above 2,6 mg/l (greater than 2 SD). These 8 sera were anti-HBc positive and one of them HBs Ag positive 3 HBs Ag positive donors had non elevated Beta 2 microglobulin levels. This survey confirms that prisoners as blood donors should be regarded as carrying a high risk of transmission of HBV and probably other infectious agents with similar epidemiology. The signification of elevated Beta 2 microglobulin levels deserves further investigations since this determination could be of value as an additive test to increase the safety of blood products.

Antibodies, Viral↗
PubMed1980

[Intraluminal diverticulum of the esophagus. Case report].

Intraluminal diverticulum of the esophagus is exceptive. It characteristic radiograhic appearance is of a smooth ovoid collection of barium surrounded by a smooth, thin radiolucent line. The diverticulum was plainly within the esophageal lumen. The diverticulum was not demonstrated on every barium study. One case of a 79 year old goitred female with intraluminal esophageal diverticulum is reported. Possible causes are considered.

Aged↗
PubMed1979

[Pancreatic pseudocysts simulating a mass in the left kidney. A report on 4 cases (author's transl)].

Pancreatic pseudocysts were observed to simulate a mass in the upper pole of the left kidney in 4 patients. Urographic investigation of these pseudocysts revealed deformities similar in all respects to those produced by a cyst, though in two cases the mass was surrounded by a thick wall. A better definition of the parenchymatous deformity was obtained by the use of true trunk profile nephrotomograms. The value of ultrasonography, using the grey scale, and of transparietal puncture with measurement of amylase levels, is stressed. The authors discuss the problem of establishing the precise location--anterior to, surrounding, or within the kidney--of these pseudocysts.

Adult↗
PubMed1977

[Inversion of intraheptic portal flow in contusions of the liver: a new concept of traumatic arterioportal fistulas (author's transl)].

Inversion of the intrahepatic flow is a phenomenon commonly observed during the initial days of a liver contusion. Its symptomatology combines one direct sign: segmentary, peripheral arterioportal reflux; and two indirect signs: arteriolar hypervascularization and a functional amputation of portal flow on selective arteriography of the superior mesenteric artery in the same territory. The direct sign may be absent, and may not be visualized in the absence of a selective injection. Both indirect signs are always present and enable the diagnosis. In most cases it is a question of a transient phenomenon secondary to a inflammatory reaction released by the contusion of the liver parenchyma; it may be the only sign of liver trauma. It may be a sign of a benign contusion and is not alone an indication for surgery. This concerns a hemodynamic phenomenon which must be clearly differentiated from arterioportal fistulas with which it is often confused.

Adult↗