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M Grandien

Publications and source records attributed to M Grandien.

At least 19 recordsLinked to original sources
PubMed1995

Association of serum antibodies against defined epitopes of human papillomavirus L1, E2, and E7 antigens and of HPV DNA with incident cervical cancer.

In order to provide a large-scale evaluation of the association with cervical cancer of antibodies against human papillomavirus (HPV) antigens, sera from 233 patients with primary, untreated cervical cancer and from 157 healthy age- and sex-matched blood donors were analyzed for IgG and IgA antibodies against HPV-derived peptide antigens and against bovine papillomavirus. Several serological responses were strongly associated with cervical cancer, notably the IgG response against the HPV 16 epitopes L1:13 (Relative risk [RR]: 5.3), E2:9 (RR: 2.9), and E7:5 (RR: 4.3), and the IgA response against an HPV 18 E2-derived antigen (245:18, RR: 3.1). HPV DNA in corresponding cervical tumors was analyzed by Southern blotting (SB) and polymerase chain reaction (PCR) in 47 patients. Sixty-six percent of the patients carried HPV DNA as determined by SB, 91% of patients analyzed by PCR. Neither the antibody responses, nor the presence of HPV DNA were significantly associated with the biological properties of the tumors.

Adult↗
PubMed1994

[Quarantine to be abolished for dogs and cats. New regulations for import of pets from EU/EFTA countries].

Owing to its natural geographical borders and strict import restrictions, Sweden has remained free from rabies since 1886. New regulations are to come into force on May 1st, 1994, permitting pet dogs and cats from EU-EFTA countries to enter Sweden without quarantine, but only after vaccination against rabies and subsequent antibody titre control. Dogs should be at least three months old before vaccination, and cats 14 months old. Antibody titres are to be tested no earlier than four months after vaccination, though in cases of re-vaccination they may be checked already after one month for dogs and after four months for cats. The rabies antibody titre should be at least 0.5 IU to permit entry of the animal into Sweden. The owner will be required to show a Swedish import permit at customs, and some means of identifying the animal will be necessary--e.g., a tattoo in the ear. Other zoonoses could also be introduced into the country by imported animals, the most feared being echinococcosis and leptospirosis. The new regulations stipulate that, in addition to being vaccinated against canine distemper, dogs should also have been vaccinated against leptospirosis less than 12 months before entry in Sweden. This does not apply to cats, but both dogs and cats should have been dewormed less than ten days before entry or re-entry into Sweden. Although the new system should effectively prevent the introduction of rabies into Sweden, an increased demand by the public for information and advice is expected.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

[BK and JC viruses--2 polyomaviruses causing disease in immunosuppressed patients].

BK and JC viruses are two polyoma viruses designated by the initials of the patients from whom they were first isolated. After the primary infection, usually occurring in childhood or early school age, the viruses become latent. Reactivation occurs during immunosuppression, and the BK virus has been shown to be the main cause of viral hemorrhagic cystitis in bone marrow transplanted patients, while the JC virus has been found to cause progressive multifocal leukoencephalopathy, PML. The paper consists in a report of results obtained with an established method for the amplification of BK and JC virus DNA. Of 20 urine specimens from patients with hemorrhagic cystitis, 13 were found to be BK virus-positive. Post-transplantation follow-up shows that the virus continues to remain detectable for long periods. JC virus positivity was found in central nervous system material (a cerebrospinal spinal fluid specimen in one case) from two PML patients.

BK Virus↗
PubMed1991

[Influenza and death. High mortality not only due to the great epidemics].

Excess mortality in a population is one of the hallmarks of an influenza epidemic. Apart from the pandemic years, 1918-1919, 1957 and 1968-1969, annual excess mortality has occurred 16 times in Sweden during the period 1911-1988. An unusually high mortality was caused by Influenza A Sichuan 2/87 (H3N2), the unexpected appearance of which at the beginning of November 1988 started an intensive epidemic in Sweden, while other European countries had a relatively mild influenza season. Swedish deaths in December totalled 10,500-2,200 more than expected; age groups over 65 years accounted for 95 per cent of the mortality, and those over 80 (N = 350,000 in Sweden) for 1,400 deaths. 0.4 percent of those over 80 died. The excess mortality is to be explained by the high proportion of the elderly in the Swedish population and the very low rate of influenza vaccination before the outbreak. As the increase in the proportion of the very elderly can be expected to continue, future influenza epidemics will result in even higher mortality unless adequate prophylactic measures are taken.

Adult↗
PubMed1991

Comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions.

Nasopharyngeal secretions obtained from 94 children with acute respiratory illness were examined for the presence of respiratory syncytial virus (RSV), adenovirus, and influenza virus type A by virus culturing (virus isolation technique [VIT]), immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). Similar results were obtained in at least two tests for RSV, influenza virus type A, and adenovirus in 92 (97.9%), 88 (93.6%), and 88 (93.6%) cases, respectively. Both rapid virus detection methods showed good specificity for the diagnosis of these virus infections (greater than or equal to 90.7%) and were more sensitive than was VIT for RSV detection. In a more accurate statistical analysis, the indexes of agreement between VIT and ELISA were substantial for RSV (kappa = 0.69; zeta = 5.5; P less than 0.0001), influenza virus type A (kappa = 0.67; zeta = 5.3; P less than 0.0001), and adenovirus (kappa = 0.71; zeta = 6.0; P less than 0.0001), while it was almost perfect for RSV when ELISA was compared with IFA (kappa = 0.88; zeta = 5.7; P less than 0.0001). Although the observed agreement was good in the comparison of these two tests for these three viruses (89%0, the indexes of agreement were moderate in the comparison of IFA and VIT for RSV (K = 0.55; Z = 2.0; P < 0.05), influenza virus type A (K = 0.42; Z = 9.7; P < 0.0001), and adenovirus (K = 0.41; Z = 6.5; P < 0.0001) and of ELISA and IFA for influenza virus type A (K = 0.55; Z = 7.0; P < 0.0001) and adenovirus (K = 0.59; Z = 6.8; P < 0.0001). All of the statistical evaluations demonstrated better agreement between ELISA and VIT for influenza virus type A and adenovirus.

Adenoviruses, Human↗