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Biomedical subjects

M Greiner

Publications and source records attributed to M Greiner.

At least 73 records · Page 4Linked to original sources
PubMed1993

[Serologic diagnosis of canine leishmaniasis: the dot-ELISA as a simple alternative to conventional ELISA].

Serology is an important tool for the diagnosis of canine leishmaniasis, since the direct detection of the parasite remains negative in many cases. A dot-ELISA was developed, which can be read without using a photometer. For this purpose assay strips were prepared, which were divided into a control field (staining indicates normal function of reagents) and four reaction fields. The reaction fields were coated with four different concentrations of Leishmania antigen and allowed semiquantitative readings of test results. The comparison of the reactions of 73 sera (20 healthy dogs, 24 dogs with leishmaniasis, 15 dogs with trypanosomiasis and 14 dogs with babesiosis) in the dot-ELISA with the results of a conventional microtiter plate ELISA showed a significant correlation between the two methods.

Animals↗
PubMed1991

Fermentation and isolation of herbicolin A, a peptide antibiotic produced by Erwinia herbicola strain A 111.

Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-l stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.

Amino Acid Sequence↗
PubMed1991

Identification of enterobactin and linear dihydroxybenzoylserine compounds by HPLC and ion spray mass spectrometry (LC/MS and MS/MS).

The production of catecholate siderophores was studied in some selected species of Enterobacter (Enterobacteriaceae). The extracted catecholates were separated as iron-free compounds by HPLC on a C18 reversed-phase column using methanol/0.1% phosphoric acid or methanol/0.1% formic acid as a solvent system and identified by ion spray mass spectrometry (LC/MS, MS/MS). Five catecholate compounds were identified which include 2,3-dihydroxybenzoylserine, its linear dimer and trimer, the cyclic enterobactin and an unidentified isomer of enterobactin. In addition, a new large-scale method for the isolation of catecholate siderphores is described which is based on adsorption on XAD-2 and subsequent purification on Sephadex LH20.

Biopolymers↗
PubMed1985

A poison prevention program for new mothers.

A program was developed to teach new parents about poison prevention and to introduce syrup of ipecac into their homes. Although the incidence of accidental poisonings is low in newborns, the objective of the program was to educate the parents before the child reaches the age when poison accidents are more likely to occur. To accomplish this a special packet was created which contained poison prevention literature and a redeemable coupon for a free one ounce bottle of syrup of ipecac. The packets were distributed to the new mothers during the postpartum hospital stay by the obstetrical nursing staff. Over 10,000 new mothers received the packets during a 10-month period. This program was funded by a grant from the Hennepin County Community Health Department and the syrup of ipecac was donated by a local retail pharmacy chain.

Female↗
PubMed1984

Gas transport during oscillatory flow in a network of branching tubes.

A transport coefficient was measured for a range of oscillatory flow conditions in a branching network of tubes. Measurements were made both across the first generation of a three-generation network and the second generation of a four-generation network. The results for these two series of tests were similar, indicating that there was no significant effect due to the system boundaries. The results are cast in terms of an effective axial diffusion coefficient of the form (Formula: see text) where kappa is the molecular diffusivity, Vt is the local stroke volume (cc); and f is the oscillation frequency (Hz). These results are compared to those obtained by other investigators in branching systems of similar geometry. At low frequency, this result is found to be in approximate agreement with the steady flow result of Scherer, et al. [15]. This expression differs from the oscillatory flow results of Tarbell, et al. [19] for liquids, primarily in terms of the effects of oscillation frequency.

Animals↗