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Biomedical subjects

M Grey

Publications and source records attributed to M Grey.

At least 55 records · Page 3Linked to original sources
PubMed1993

The in vivo effects of PIXY321 therapy on human monocyte activity.

In this report we describe the time-dependent effects of PIXY321 (a synthetic hybrid cytokine) treatment (500 and 750 micrograms/m2/day for 14 days) on six sarcoma patients. Blood was taken prior to PIXY321 injection (day 0), on days 1, 7, and 14 of treatment, and 7 days posttreatment (day 21). The number of isolated monocytes quadrupuled by day 7 and sustained a significant increase through day 14. There were significant increases in the percentage of circulating monocytes relative to total mononuclear cells on days 1 and 7 of therapy. There were no significant changes in monocyte cell surface antigens (15 checked), suggesting that the increase in monocyte numbers was not due to increased numbers of immature monocytes. The basal activity of the monocytes was not markedly altered during treatment; however, they were primed for significantly increased phorbol 12,13-dibutyrate-stimulated superoxide anion production and endotoxin-stimulated release of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) on days of 1 and 7 of therapy. There was a significant increase of IL-1 beta mRNA levels (unstimulated cells) on days 1 and 7, but TNF-alpha mRNA levels increased significantly on day 1 only. Consistent with the increase in superoxide anion production, there were increases in monocyte protein kinase C (PKC) levels on all days of therapy. There was a significant increase in PKCII beta mRNA only on the first day of treatment. All significant changes in monocyte number and function produced by PIXY321 infusion were reversible, as there were no sustained effects on day 21 (7 days after therapy). These results indicate that the effects of PIXY321 may be mediated through up-regulation of PKC resulting in monocytes primed for increased functional activity in response to an appropriate second stimulus.

Antigens, CD↗
PubMed1993

Establishing a continence service using AHCPR guidelines.

The Agency for Health Care Policy and Research (AHCPR) has recently released practice guidelines for the management of adult urinary incontinence. The guidelines are designed to enhance the quality, effectiveness and appropriateness of health care in the area of incontinence. The purpose of this article is to describe assessment and management strategies as used in a continence clinic that incorporate AHCPR guidelines. This continence clinic exists within an established gynecology practice, however, continence services can be provided in nursing homes and/or home care agencies.

Female↗
PubMed1993

Stressors and children's health.

This article reviews the literature on the association of stressors and physical and psychological health in childhood. These relationships are considered from a multidisciplinary perspective. Many studies have been published comparing children experiencing a large number of life events with those who experience fewer, with the consistent finding that those with more stressful experiences are significantly more likely to experience illness, hospitalization, or use health services. Although a large body of research exists, a clear picture of the nature and strength of this relationship has not yet emerged. Clarification of the psychophysiological pathways responsible for the associations reported may be important to our understanding of these issues. Such pathways may have their origin in the alteration of immune function. Further research aimed at explication of the complex relationships between the neurological, endocrine, and immune systems and behaviors will need to be multifactorial. Such research has the potential to improve our understanding of psychosomatic phenomena and lead to more effective nursing care.

Child↗
PubMed1992

A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation.

We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.

DNA, Fungal↗
PubMed1992

Rapid and simple isolation of DNA from agarose gels.

We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.

Cloning, Molecular↗
PubMed1992

Children's participation in research: their right to consent.

This article discusses the ethical and legal considerations related to the inclusion of children in the research process. Specific attention is paid to situations in which customary guidelines are inappropriate. In addition, the results of a survey to determine the manner in which Institutional Review Boards are addressing these issues is presented with suggestions to assist nurse researchers in acquiring review board approvals.

Child↗
PubMed1991

Adaptation to chronic illness in childhood: diabetes mellitus.

The purpose of this paper is to discuss a conceptual frame of reference for the study of human responses to chronic illness in childhood using diabetes mellitus as a model case and to review the research findings on physiological and psychological adaptation. In addition, the implications of these studies for nursing practice are presented.

Adaptation, Physiological↗
PubMed1979

Impaired parathyroid response to induced hypocalcemia in thalassemia major.

Ethylene diamine tetra-acetic acid-induced hypocalcemia was used as provocative test of parathyroid reserve in eight normocalcemic patients with thalassemia major (age 8 to 26 years) and five young adult control subjects (age 22 to 35). In response to an intravenous infusion of disodium EDTA (50 mg/kg), serum immunoreactive parathyroid hormone rose by 1.97 +/- 1.93 (SD) microliterEq/ml in the patients, controls showing a rise of 10.6 +/- 3.6 microliterEq/ml (t = 5.46, P less than 0.001). There was no relationship between parathyroid response and total iron burden as measured by serum ferritin- or desferrioxamine-induced urinary iron excretion. Impairment of parathyroid reserve is common in transfused patients with thalassemia major and may serve as a marker of significant iron overload.

Adolescent↗
PubMedDate not supplied

1988 NAPNAP membership survey: characteristics of members' practice.

In 1988 the characteristics of the 2560 members of NAPNAP were determined by means of a mailed survey. Approximately 52% of the members returned usable questionnaires. Members were asked for information about their educational preparation, demographic characteristics, and professional employment status. Data about members' practice, how their practice has changed over time where possible, and implications for the future are reported.

Data Collection↗
PubMedDate not supplied

Future potential, phase I: nurse practitioners look at themselves.

Nurse practitioners identified as successful were interviewed to evaluate factors related to the viability of nurse practitioners in the health care system. The interviewees believed they were successful because they provided a nursing dimension of primary care, developed strategies to market themselves and their services, collaborated with physicians who saw them as colleagues, and had certain personality characteristics. In addition, the interviewees suggested strategies to enhance practice and potential nurse practitioner roles. These results suggest further research in two areas: investigation of health care administrators and management to solicit their ideas about the future strategic issues for the development of nurse practitioner roles and a broader, more formal survey of nurse practitioners regarding their success in the marketplace.

Career Choice↗
PubMedDate not supplied

1988 NAPNAP membership survey. Part II: characteristics of the members.

In 1988, the 2560 members of NAPNAP were surveyed about their educational preparation, demographic characteristics, and professional employment status. Questions were included on members' opinions about issues that should be emphasized by NAPNAP in the future. Approximately 52% of the members returned usable questionnaires. In this second part of the survey results, we report the characteristics of members, how these characteristics have changed over time, and what the members believe NAPNAP's future priorities should be.

Adult↗