PubMed Health⌕ Search

Biomedical subjects

M Griessen

Publications and source records attributed to M Griessen.

33 records · Page 2Linked to original sources
PubMed1981

Significance of duodenal disaccharidases. A comparative study of duodenal and jejunal values.

Endoscopic biopsies at the inferior duodenal flexure from 36 patients were assayed for disaccharidases. Comparative studies in 8 patients were carried out from this location and from the duodenojejunal flexure. 16 patients with lactase values under 20 U/g protein and normal histology also underwent a lactose tolerance test. Evidence is presented that the easily performed duodenal biopsies give the same information as those from the angle of Treitz, at a level about 40% under the latter. Tolerance to lactose was in close relation with the lactase level in 16 patients, the limit being at about 5 U/g protein.

Biopsy↗
PubMed1980

Antibody diversity in amphibians: evidence for the inheritance of idiotypic specificities in isogenic Xenopus.

Two families of isogenic Xenopus and the progeny from one individual of each family were immunized with dinitrophenylated keyhole limpet hemocyanin. Antisera were raised in mice and in guinea pigs against purified antibodies from one individual of the first generation of each family. These were rendered anti-idiotypic by absorption with immunoglobulins of unimmunized isogenic frogs. In the first generation, antibodies from individuals of each family exhibited a high degree of idiotypic cross-reactivity. Antibodies from individuals of the second generation displayed idiotypic cross-reactivity, but to a lesser extent. Some idiotypic cross-reactivity was seen between members of one family with members of the other family: no sharing of idiotypic specificity was detected among anti-DNP antibodies isolated from individual outbred frogs. Isoelectric focusing (IEF) patterns of the anti-DNP antisera showed a high degree of restriction and overlap within members of each family and across two generations. Furthermore, the N-terminal sequence of the L chains from X. laevis anti-DNP antibodies was unique, and there was a low degree of structural heterogeneity in the H chain N-terminal region. A correlation between the IEF banding pattern and idiotypic reactivity was apparent. The data suggest the inheritance of variable region genes through two generations of isogenic Xenopus.

Amino Acid Sequence↗
PubMed1979

[Exhaled hydrogen test in the detection of lactase deficiency].

To determine the validity of breath H2 measurements in detecting lactase deficiency, capillary blood glucose and breath H2 were measured after ingestion of 50 g lactose in 34 patients with abdominal symptoms or diarrhea. 18 patients had biopsy-proven lactase deficiency and 16 normal lactase activity. Blood glucose determination produced 2 false-positive results while breath H2 measurements provided 2 false-negative and 4 false-positive results.

Adult↗
PubMed1978

Effect of Ca2+, Mg2+, NaN3, cholinergic agents, and gastrointestinal hormones on the guanylate cyclase from guinea pig gastric mucosa.

Guanylate cyclase in the guinea pig fundic mucosa occurred in two enzymatic forms: a "soluble" form and a particulate form. The mean basal activity of the soluble fraction measured in the presence of 300 micrometer guanosine-5'-triphosphate and 5 mM MnCl2 was 72.6 +/- 5.3 pmoles of cyclic GMP per mg of protein per min. Guanylate cyclase activity was dependent on Mn2+; it was increased by sodium azide (NaN3), CaCl2, cysteine, secretin, and cholecystokinin, but it was not influenced by gastrin, histamine, cholinergic esters, prostaglandins E1 and A1. NaN3 (1 mM) decreased the apparent Km for MnCl2 and potentiated the effects of MgCl2. The activity of the particulate fraction represented about 14% of that of the supernatant fraction. The guanylate cyclase activity of that fraction was not modified by NaN3, gastrin, cholinergic agents, secretin, or cholecystokinin. Cysteine inhibited its activity. These data do not support the hypothesis that cyclic GMP acts as a second messenger for the action of cholinergic agents and gastrin in the guinea pig gastric mucosa.

Animals↗
PubMed1977

[The intestines: an endocrine gland with multiple secretions].

The endocrinology of the digestive system has been a stimulating area of research for 15 years. Studies have shown that the intestine, with the many hormones it secretes, is to be considered an endocrine gland per se. The questions surrounding the nature, origin and functions of these hormones, and their role in human clinical medicine, are discussed. The answers, although incomplete and provisional, open highly interesting perspectives for the gastroenterologist.

Cholecystokinin↗
PubMed1976

Action of H1 and H2 inhibitors on the response of histamine sensitive adenyly cyclase from guinea-pig mucosa.

In the guinea-pig, it has been shown that homogenates of mucosa from the fundus contain an adenylyl cyclase system that is activated by histamine as well as by prostaglandins PGE1 and PGA1. The effects of burimamide, an H2-inhibitor, and mepyramine and chlorpheniramide, both H1-inhibitors, were tested. Both H1 and H2 inhibitors behaved kinetically as competitive inhibitors of histamine, but the Km derived for burimamide (2.5 - 4.1 . 10(-5)) was significantly lower than that for either chlorpheniramine (0.9 - 1.9 . 10(-4)) or mepyramine (1.3 - 1.4 . 10(-4)). On the other hand none of the three inhibitors influenced the cyclase activation by PGE1 and PGA1. These results suggest that there are at least two types of receptors in the preparation studied, one responsive to histamine and the other to the prostaglandins, and that the specificity of H1- and H2-receptors is not absolute in the broken cell preparation.

Adenylyl Cyclases↗
PubMed1975

A strategy of approaching the fit of a compartmental model applied to the behaviour of aspirins in humans.

A compartmental model has been set up with five parts. Formulation and testing of the model have been performed by simultaneous experiments involving measurement of blood levels of ASA and SA for four different preparations of aspirin investigated in four different patients. The hypotheses of the present study had been based upon a four-compartment model. However, such a model did not appear to be adequate and a new model with five compartments has been substituted for the former one. The five-compartment model has led to the formulation and testing of new physiological hypotheses; it has also provided the development of a fitting method using powerful convergence algorithms. A general analytical formulation has been set up which has made it possible to circumscribe the multiple roots of the characteristic polynomial when they have to be taken into consideration.

Aspirin↗