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Biomedical subjects

M Gripenberg

Publications and source records attributed to M Gripenberg.

At least 37 records · Page 2Linked to original sources
PubMed1984

Demonstration of anti-keratin antibodies by ELISA using keratin or thiol-containing compounds in urea as antigens.

During the development of an enzyme-linked immunosorbent assay (ELISA) for the demonstration of anti-keratin antibodies (AKA) it was observed that rabbit anti-keratin antisera also reacted with polystyrene surfaces treated with beta-mercaptoethanol in 8 M urea (ME-urea). Sera from non-immunized rabbits or rabbits immunized with antigens unrelated to keratin failed to react. The specificity of the reaction was further assessed by absorption experiments and by testing affinity-purified AKA. IgM activity against ME-urea could be demonstrated in 62.5% of sera from patients with infectious mononucleosis and in 37.5% of sera from patients with rheumatoid arthritis, and there was a good correlation to the presence of AKA. Coating of the solid phase with compounds containing free SH groups in 4-8 M urea generated the antigen of this ELISA. The exact molecular configuration of this presumptive synthetic antigen is obscure, but the ME-urea ELISA seems to provide a simple way to detect anti-keratin antibodies of a certain specificity.

Animals↗
PubMed1984

Enzyme-linked immunosorbent assays for antibodies to poly(A), poly dAT and histones: possibly useful tools for the evaluation of prognosis and disease activity in systemic lupus erythematosus.

In a prospective study 222 sera from 56 patients with systemic lupus erythematosus (SLE) were tested for antibodies to poly(A), poly dAT and histones using enzyme-linked immunosorbent assay (ELISA). Patients with active disease had significantly more often antibodies to poly(A), poly dAT and histones than patients with inactive disease. There was a positive correlation between the activity score of the disease and the levels of poly(A)-antibodies of IgG and IgM class, poly dAT-antibodies and antibodies to histones. Patients with SLE-nephritis had a higher level of poly dAT and IgG class poly(A) antibodies than patients without nephritis. Interestingly, patients with an SLE-nephritis had lower levels of IgM-poly(A)-antibodies than those without nephritis. Attempts to use the ELISAs in predicting the SLE exacerbations were unsuccessful. However, the assays can be used as parameters in the estimation of the disease activity.

Adolescent↗
PubMed1984

The usability of the MRL/1 mouse strain in detection of anti-rheumatic drugs.

D-Penicillamine (5 mg/kg), prednisolone (0.5 mg/kg), tolfenamic acid and thiabendazole (10 mg/kg) were chronically administered to male and female mice of MRL/1 strain. The treatment was started either at 4 weeks of age (prophylactic dosing) or at 12 weeks of age (therapeutic dosing) and continued until the termination at 24 weeks of age. In male mice all the drugs caused a significant retardation in IgM-RF formation with one of the dosing regimes. D-Penicillamine and prednisolone also had an inhibitory effect on lymph node enlargement. In female mice, however, only thiabendazole retarded the RF activity significantly. None of the drugs had any marked effect on anti-ssDNA antibody formation, and none of the treatments affected the development of renal disease or modified the survival of these mice.

Animals↗
PubMed1984

Profiles of antinuclear antibodies in chronic active hepatitis, primary biliary cirrhosis and alcoholic liver disease.

The profiles of specific antinuclear antibodies were determined in sera from 23 patients with the idiopathic type of chronic active hepatitis (CAH), 15 patients with primary biliary cirrhosis (PBC) and 25 patients with alcoholic liver disease (ALD). The indirect immunofluorescence test for antinuclear antibodies using cultured human embryonic fibroblasts as substrate was positive in 78% in CAH, in 73% in PBC and in 24% in ALD. Seventeen percent of CAH sera and 33% of PBC sera stained small speckles in interphase nuclei. This staining pattern probably represents a new subset of ANA as the centromeres (kinetochores) were not stained. Antibodies to native DNA by the Crithidia luciliae test were found in only one serum from a patient with CAH. In addition, 17 percent of the CAH sera reacted with the saline extract of rabbit thymus by double immunodiffusion. Antibodies to the Sm- or RNP-antigens were not found. SS-B antibodies could be demonstrated in 39% of the CAH sera by a sensitive immunoenzymatic technique. Patients with CAH also had significantly higher levels of antibodies against denatured, single-stranded DNA (ss-DNA) and a synthetic RNA molecule, poly(A) as compared to other groups. Patients with an atypical cholestatic CAH had an antinuclear-antibody profile resembling that of the other CAH patients, but different from that of PBC patients. Patients with alcoholic cirrhosis had significantly higher levels of ss-DNA- and poly(A)-antibodies than other patients with ALD. It is concluded that the determination of an antinuclear-antibody profile using the ELISA seems to be clinically useful in the classification of chronic liver diseases.

Adult↗
PubMed1984

Anti-ribonucleoprotein antibodies in inflammatory rheumatic diseases.

Of 472 consecutive patients with inflammatory rheumatic diseases attending a unit responsible for the treatment of rheumatoid diseases, only 12 patients were found to have anti-ribonucleoprotein (RNP) antibodies. Of these, 5 patients fulfilled the criteria for the diagnosis of systemic lupus erythematosus (SLE) and 7 those of rheumatoid arthritis. Joint symptoms were the predominant clinical features and caused severe deformities requiring operations in most cases. One anti-RNP-positive SLE patient died of chronic active hepatitis.

Adult↗
PubMed1984

Circulating immune complexes in Yersinia infection.

The occurrence of circulating immune complexes (CIC) was studied in 9 patients with Yersinia arthritis and 7 with Yersinia enteritis (YE) without extraintestinal symptoms by the platelet-iodinated protein A (PIPA) technique and by a solid phase Clq-binding enzyme-linked immunosorbent assay. CIC were detected by both techniques in all the patients with Yersinia arthritis during the acute disease and in 3 of them after recovery. CIC were also found in 5 of the 7 patients with uncomplicated YE, suggesting that CIC were associated with YE irrespective of the development of arthritis.

Adolescent↗
PubMed1983

Expression of antibody activity measured by ELISA. Anti-ssDNA antibody activity characterized by the shape of the dose-response curve.

To demonstrate the presence or absence of antibodies, results derived from a single serum dilution in an ELISA are sufficient. However, qualitative differences in antibodies are reflected by the shape of dose-response curves. A method based on approximating the absorbance value by a polynomial p(x) = a1x + a2x2, where 1/x is the dilution factor, was used to characterize the dose-response curves in an ELISA for anti=ssDNA antibodies. The parameters used are E = a1 and A = -a2(1)/a2. It can be argues that E gives an estimate of the effective amount of antibodies in the serum and that A is essentially a function of the reaction constants between antibody and antigen.

Antibody Affinity↗
PubMed1982

Purification and characterization of a nuclear SS-B antigen.

A nuclear SS-B antigen was isolated from a saline extract of acetone powder of rabbit thymus by precipitation with ammonium sulphate, affinity chromatography with Blue Sepharose CL-6B, and preparative agarose gel electrophoresis. The mol. wt of the antigen was 68,000. Its electrophoretic mobility was similar to that of pre-albumin, and the iso-electric point was around pH 4.0. The main amino acids of the antigen were glutamic acid, leucine, lysine and alanine. Both histidine and tyrosine were also found. The purified antigen precipitated with anti-SS-B sera but not with any other reference antisera. It resembled La and Ha antigens in susceptibility to proteolytic and nucleolytic enzymes and to heat. The purified SS-B antigen, however, had a higher molecular weight than did the Ha and La antigens. The molecule could not be split into subunits with mercaptoethanol or acid. Counter-electrophoresis showed antibodies to the SS-B antigen in sera from patients with rheumatic diseases, including rheumatoid arthritis, systemic lupus erythematosus and Sjögren's syndrome, but not in amy of the control sera.

Adult↗
PubMed1981

Common serological features in rheumatoid arthritis and yersinia arthritis. Demonstration of rheumatoid factors and antibodies against ssDNA and Yersinia enterocolitica Lipopolysaccharide by ELISA.

Sera from 31 patients with rheumatoid arthritis (RA) and from 49 patients with Yersinia enterocolitica 0:3 infection were examined for the presence of rheumatoid factors (RF) and antibodies against denatured (ss) DNA and Y. enterocolitica 0:3 lipopolysaccharide (LPS), by using enzyme-linked immunosorbent assays. Anti-ssDNA antibodies of IgG class were found equally often in the RA as in yersinia patients. The IgM-anti-ssDNA antibody levels were also elevated in the yersinia patients, whereas the RA patients had elevated levels of IgA-anti-ssDNA antibodies. High levels of RF were seen in the RA patients and an increase in IgA-RF activity was observed during the acute stage in the yersinia arthritis patients. An overlap in the serological profiles of RA and yersinia arthritis was further seen in th results of the anti-Y. enterocolitica LPS determinations, a anti-LPS antibodies of IgG class were present not only in yersiniosis but also in 16% of the RA patients.

Adolescent↗
PubMed1981

Mixed connective tissue disease: a follow-up study of 12 patients with special reference to cold sensitivity and skin manifestations.

A great variety of skin manifestations and the frequent occurrence of cold sensitivity, vascular symptoms with peripheral painful ulcerations, headache of migraine type, and muscle and joint symptoms are described in a series of 12 patients with an overlap syndrome compatible with mixed connective tissue disease (MCTD). The patients have been followed up for an average of 7 years. The peripheral symptoms on the extremities in particular were exacerbated by exposure to cold and caused much inconvenience and early disability for the patients. In addition to the symptoms generally connected with MCTD, some of the patients presented signs of other diseases of autoimmune type as well. For instance 2 of the patients presented autoimmune thyroiditis and one patient developed myasthenia gravis and cold agglutinin syndrome.

Adolescent↗
PubMed1981

Antibodies against polyriboadenylic acid in systemic lupus erythematosus and rheumatoid arthritis demonstrated by ELISA. Prevalence of and relation to antibodies against DNA and extractable nuclear antigens.

An enzyme-linked immunosorbent assay (ELISA) for the demonstration of class-specific antibodies against polyriboadenylic acid (poly-(A)) is described. The results revealed the presence of IgG-anti-poly(A) antibodies in 63% (22/35) of patients with systemic lupus erythematosus (SLE) and in 25% (22/88) of patients with rheumatoid arthritis (RA), the mean antibody level being significantly higher in the SLE patients. Anti-poly(A) antibodies of IgM class were seen in 15 and 17% of the patients, respectively. The prevalence of anti-poly(A) and anti-ssDNA antibodies of IgG class was similar in the patients with SLE, but the occurrence of antibodies against poly(A), DNA and extractable nuclear antigens varied independently of each other. In the RA-patients, a positive correlation was seen between the levels of IgG-anti-ssDNA and IgG-anti-poly(A) antibodies.

Adolescent↗
PubMed1979

Demonstration of antibodies against Yersinia enterocolitica lipopolysaccharide in human sera by enzyme-linked immunosorbent assay.

Antibodies against Yersinia enterocolitica serotype O:3 lipopolysaccharide present in sera from patients with Yersinia infection were studied by using an enzyme-linked immunosorbent assay. Of the sera with significant bacterial agglutination titers against Y. enterocolitica type O:3, 86% contained anti-lipopolysaccharide antibodies of the immunoglobulin G class. With the sera of some patients, we demonstrated increasing anti-lipopolysaccharide antibody levels of immunoglobulin G class in spite of decreasing bacterial agglutination titers. The assay was specific for lipopolysaccharide from Y. enterocolitica type O:3, and in inhibition experiments lipopolysaccharide could be detected in amounts of greater than or equal to 0.5 micrograms/ml.

Agglutination Tests↗
PubMed1978

A solid phase enzyme-linked immunosurbent assay (ELISA) for the demonstration of antibodies against denatured, single-stranded DNA in patient sera.

A solid phase enzyme-linked-immunosorbent assay (ELISA) for the determination of antibodies against denatured, single-stranded (ss-) DNA is described. Polystyrene cuvettes coated with ss-DNA were incubated with serum samples and the anti-ss-DNA antibodies bound were detected by means of an anti-IgG-alkaline phosphatase conjugate. The binding of anti-ss-DNA antibodies in individual sera was expressed as units calculated as % of the absorbance in relation to the absorbance value obtained with a reference pool. Absorption experiments showed that the assay is specific for antibodies against denatured DNA. By using immunologically purified anti-ss-DNA antibodies the assay was shown to detect specific antibodies in concentrations down to 1 ng/ml. Antibodies against DNA could be detected in 94% of sera with antinuclear antibodies.

Antibodies, Antinuclear↗
PubMedDate not supplied

Humoral immune stimulation and antiepithelial antibodies in Yersinia infection.

Results consistent with a general humoral immune stimulation were found when 127 sera from 89 patients with Yersinia enterocolitica infection were studied. Significantly increased gammaglobulin concentrations and elevated isohemagglutinin titers were seen in these sera as compared to sera from normal blood donors and patients with streptococcal infection. Antinuclear and anti-smooth muscle antibodies were demonstrated in both yersinia and streptococcal infection. The prevalence of non-organ specific antiepithelial antibodies reacting with gastrointestinal and thyroid epithelial cells was significantly increased in yersinia infection.

Adolescent↗