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Biomedical subjects

M Grodin

Publications and source records attributed to M Grodin.

8 recordsLinked to original sources
PubMed1988

Ontogenesis of epidermal growth factor in liver of BALB mice.

To characterize the ontogenesis of hepatic epidermal growth factor (EGF) metabolism in normal BALB mice, we measured serum and liver concentrations of EGF and liver concentrations of pre-pro EGF mRNA. Female and male animals were studied at 1, 2, 5, 7, and 10 wk of life. After death, body weight and length were measured, and serum and liver tissues were collected for EGF determinations. Immunoreactive serum EGF (means +/- SE) increased at 7 and 10 wk and was significantly higher (P less than 0.05) in males (465 +/- 58 and 683 +/- 120 pg/ml) than females (188 +/- 52 and 295 +/- 64 pg/ml). Liver EGF concentrations were low at 1, 2, and 5 wk, significantly increasing (P less than 0.01) at 10 wk to 179 +/- 36 vs. 268 +/- 49 pg/mg protein for females and males, respectively (female and male values were significantly different, P less than 0.01). Pre-pro EGF mRNA was examined at 1, 2, 3, 5, 7 and 10 wk. EGF message increased in liver to highest values at 10 wk in both males and females. There was a high correlation between serum and liver EGF concentrations during the first 10 wk (r = 0.97 and 0.85 for males and females, respectively) and a twofold increase in liver EGF mRNA between 3 and 10 wk of postnatal life. These results suggest that liver may be an important source of circulating EGF in developing BALB mice.

Animals↗
PubMed1983

Pediatric cardiac emergencies.

The clinical and laboratory findings in and the treatment of congestive heart failure, supraventricular tachycardia, pericardial disease, and hypoxemic spells are discussed.

Child↗
PubMed1980

Chemically-defined organ culture of embryonic mouse tooth organs: morphogenesis, dentinogenesis and amelogenesis.

Chemically-defined in vitro conditions without serum have been identified which are permissive for embryonic mouse tooth organs to express morphogenesis and cytodifferentiation. In the absence of antibiotics, embryonic extracts, autologous and/or heterologous sera, molar tooth organs from Theiler stage 25 embryos (C57BL/6, A/Jax and Tabby mouse strains) routinely formed discrete dentin and enamel extracellular organic matrices within 10 days of continuous in vitro cultivation. Collagen and non-collagenous protein synthesis and secretion by differentiated cells within explants, between 9 and 10 days in vitro, were studied with light microscopic autoradiography. Finally, explants of embryonic mouse molar tooth organs have been maintained in organ culture for three week without serum supplementation.

Amelogenesis↗