Biomedical subjects

M H Curfs

Publications and source records attributed to M H Curfs.

6 recordsLinked to original sources

PubMed1996

Induction of c-fos expression in cervical spinal interneurons after kainate stimulation of the motor cortex in the rat.

The expression of the immediate-early gene c-fos was used as a marker of neuronal activity to investigate the cervical spinal interneuron populations involved in the corticomotoneuronal pathway. Adult rats received unilateral kainate injections in the forelimb area of the primary motor cortex. After a survival period of 90 min, during which the animals showed vehement twitching of the contralateral forelimb, the rats were perfused and their brains and cervical spinal cords processed for Fos-like immunoreactivity. In the cervical spinal cord Fos-like immunoreactive neurons were found bilaterally in the dorsal horn and in the intermediate zone, though contralaterally significantly more labelled nuclei were encountered in two different areas. One area closely resembles the corticospinal terminal field as demonstrated with anterograde horseradish-peroxidase tract-tracing and the other reflecting primary afferent and noxious sensory neurons in the dorsal horn. Thus by monitoring the evoked expression of the immediate-early gene c-fos, structural components of the rat motor system can be identified.

Animals↗

PubMed1996

Direct cortico-motoneuronal synaptic contacts are present in the adult rat cervical spinal cord and are first established at postnatal day 7.

In order to demonstrate direct cortico-motoneuronal synaptic contacts in the cervical spinal cord of the rat and to determine at which postnatal age these contacts are established, an electron microscopic study using double labelling was performed. Corticospinal axons were anterogradely labelled after horseradish peroxidase (HRP)-gel implantation into the cerebral motor cortex and motoneurons were retrogradely labelled after cholera toxin subunit B conjugated to HRP (CTB-HRP) injections into the distal forelimb flexor muscle. With the histochemical procedures used, both tracers yield similar needle-like crystalline deposits. Labelled axons, however, can be well differentiated from labelled motoneuronal dendrites and somata on morphological grounds. In adult rats, direct cortico-motoneuronal contacts were encountered. Experiments in developing postnatal rats demonstrated that these synapses are first present on postnatal day 7.

Afferent Pathways↗

PubMed1995

Transient functional connections between the developing corticospinal tract and cervical spinal interneurons as demonstrated by c-fos immunohistochemistry.

Previous research on the rat corticospinal tract (CST) which develops mainly postnatally revealed that some CST axons grow transiently into the spinal gray matter and are subsequently eliminated. In the present study the question was addressed whether these fibres also form transient functional connections. Rats aged 14 and 60 days postnatally received unilateral injections of the potent glutamate agonist kainate into the cerebral motor cortex. After a survival period of 90 min. the rats were perfused and their brains and spinal cords processed for the immediate early gene c-fos by immunohistochemistry. Increased levels of c-fos as opposed to sham-operated animals was observed in several brain nuclei as well as in the cervical spinal cord. In the spinal gray one population of labelled interneurons in particular appeared to correlate well with the CST projection field. A decrease was noted in the number of c-fos positive neurons from postnatal day 14 to 60, suggesting that during development transient functional connections are formed between the CST and its target.

Aging↗

PubMed1994

Selective elimination of transient corticospinal projections in the rat cervical spinal cord gray matter.

In the present paper a description is given of the development of the rat corticospinal tract (CST) in the lower cervical spinal cord. This area contains, among other cells, the motoneurons innervating the distal forelimb muscles. HRP gels were implanted in the sensorimotor cortex of Wistar rats varying in age from postnatal day 0 (P0) to P60. After a survival period of 48 h, the rats were transcardially perfused, the spinal cords transversely sectioned at 30 microns and the sections reacted for HRP. Labelled CST axons in the dorsal funiculus were first detected at P2, and after a delay of 2 days the first fibres were found in the adjacent gray matter (P4). More labelled fibres were gradually added until maximal number and extension was reached at P10. By then the entire gray matter and large parts of the white matter were covered by labelled CST axons. From P10 onwards, the number of labelled CST fibres as well as their extension decreased. In the adult rat, some areas such as the lateral part of the ventral and dorsal horn and large parts of the ventral and lateral white matter ultimately became devoid of labelled CST axons. It is concluded that a massive overshoot occurs during the development of the terminal field of the rat CST. The results are discussed in conjunction with our previous findings on the development of the motoneurons innervating the rat distal forelimb muscles. The concurrent selective elimination of both CST axons and motoneuron dendrites is suggested to be correlated with progressively more mature, coordinated movements and with high digital skills especially.

Aging↗

PubMed1994

Retrograde neuronal tracing with cholera toxin B subunit: comparison of three different visualization methods.

In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb). CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively. The following results were obtained. (1) Immunochemical detection of CTb with peroxidase and DAB-Ni incubation provides the best labelling of the cell bodies and their processes, whereas immunochemical detection with FITC produces less effective labelling of the dendrites. (2) Histochemical visualization of CTb-HRP conjugate gives results similar to those of CTb immunochemistry but produces a much more granular appearance of the label, which may affect the identification of distal dendrites. In addition, direct electron-microscopic analysis of labelled structures can be achieved. (3) CTb-FITC and CTb-TRITC visualization permit double-labelling experiments but the labelled cells exhibit fluorescence only in their somata and proximal dendrites. (4) Factors other than labelling intensity, e.g. double-labelling, preservation of the label, compatibility with other techniques and even economic reasons must be taken into consideration when a selection of visualization methods is to be made.

Animals↗

PubMed1993

Postnatal maturation of the dendritic fields of motoneuron pools supplying flexor and extensor muscles of the distal forelimb in the rat.

In the rat cervical spinal cord the corticospinal projection on motoneurons either direct or indirect (via interneurons) comes about postnatally making it accessible for experimental research. Therefore, the postnatal developmental changes of motoneurons and in particular their dendritic fields were examined. Motoneurons innervating the two antagonistic muscles in the distal forepaw, the m. flexor digitorum profundus and the m. extensor digitorum communis, were retrogradely labelled by intramuscular injections of cholera toxin subunit B conjugated with horseradish peroxidase in rats of various postnatal ages. Following a 48-72 hour survival period the motoneurons and their dendritic fields were studied in the seventh and eighth cervical spinal cord segments. Both the number and the position of motoneurons were found to remain constant throughout postnatal development. Extensor motoneurons were positioned dorsolaterally in the ventral horn at the border of grey and white matter, flexor motoneurons were in general medial to extensor motoneurons. The results on the dendritic field demonstrate firstly, that during postnatal development the extension of the dendrites of both flexor and extensor motoneurons changes from spreading out in all directions at postnatal day 2 to spreading in only a few, specific directions from postnatal day 21 onwards, with the restriction that both motoneuron pools follow a different time scale to achieve this. Secondly, both pools have a temporal dendritic component extending into the white matter of the lateral funiculus.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗