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Biomedical subjects

M H Mills

Publications and source records attributed to M H Mills.

6 recordsLinked to original sources
PubMed1998

Tetanus: critical implications for nursing.

In the United States today, tetanus is a rare disease. However, when it occurs, it can be a highly challenging situation for all members of the healthcare team. The majority of care during the course of the disease is supportive. The availability of personnel trained in managing cardiorespiratory emergencies and sustained mechanical ventilation in the ICU environment can have a beneficial effect on patients' outcomes. An awareness of the potential complications of tetanus can hasten recovery and maintain the patient's preexisting quality of life.

Critical Care↗
PubMed1994

Determination of ketorolac enantiomers in plasma using enantioselective liquid chromatography on an alpha 1-acid glycoprotein chiral stationary phase and ultraviolet detection.

A chirally selective high-performance liquid chromatographic assay was developed to measure the R(+) and S(-) enantiomers of ketorolac in plasma for pharmacokinetic studies. Naproxen sodium [S(+) enantiomer] (10 micrograms) was used as an internal standard. Plasma samples (0.5 ml) were acidified (50 microliters of 4 M H3PO4 to pH 1.5), extracted into 0.4 ml of 10% pentan-2-ol in hexane and back-extracted into 0.15 ml of base (20 mM NaOH pH to 7-8), of which samples (5 microliters) were chromatographed on a 100 x 4 mm I.D. column packed with an HPLC chiral stationary phase based upon immobilized alpha 1-acid glycoprotein (Chiral AGP-CSP) with 4% propan-2-ol in 0.1 M NaH2PO4 pH 5.5, at 0.9 ml/min. Detection was at 325 nm and run time was 10 min. Retention times of R- and S-ketorolac and of S(+)-naproxen were 3.3, 4.8 and 6.4 min, respectively. The metabolite p-hydroxyketorolac was not resolved enantiomerically and had a retention time of 2.2 min. The assay was linear over the range 0.5-10 mg/l, with precisions < 5% C.V. Good separations (alpha > 1.35) and resolutions (Rs > 3.23) between peaks were achieved. The sensitivity could be extended to 35 micrograms/l with less precision by increasing the injection volume to 100 microliters.

Chromatography, High Pressure Liquid↗
PubMed1991

Determination of melatonin and monoamines in rat pineal using reversed-phase ion-interaction chromatography with fluorescence detection.

A method is reported for the ion-interaction, reversed-phase separation of 24 compounds (chiefly monoamines) arising from the metabolism of tyrosine and tryptophan. These compounds were separated as two groups. The first group comprised 3,4-dihydroxyphenylethylene glycol, tyrosine, 3-methoxy-4-hydroxyphenyl glycol, 5-hydroxytryptophan, norepinephrine, 3,4-dihydroxyphenylacetic acid, epinephrine, 5-hydroxyindole-3-acetic acid, homovanillic acid, 5-hydroxytryptophol, dopamine, tryptophan. N-acetylserotonin, N-acetyltryptophan, 5-methoxytryptophan and serotonin. The mobile phase consisted of a 6.8:93.2 (v/v) mixture of acetonitrile and an aqueous solution containing 0.16 M ammonium phosphate, 0.06 M citric acid, 0.15 mM disodium EDTA, 10 mM dibutylamine and 6 mM sodium 1-octanesulphonate at pH 4.50. The second group of compounds comprised 6-hydroxymelatonin, 5-methoxyindole-3-acetic acid, indole-3-acetic acid, 5-methoxytryptamine, tryptamine, 5-methoxytryptophol, melatonin and tryptophol. The mobile phase consisted of a 16:84 (v/v) mixture of acetonitrile and an aqueous solution containing 0.05 M ammonium phosphate, 0.05 M citric acid, 0.15 mM disodium EDTA, 25 mM dibutylamine and 5 mM sodium 1-octanesulphonate at pH 5.30. Detection was by fluorescence measurement (lambda ex = 280 nm, lambda em = 340 nm). The proposed method exhibited linear calibration over the biochemically significant concentration range, with detection limits in the 10-200 pg range. Excellent precision for peak areas and retention times was observed, even over a period of 24 h. The applicability of amperometric detection (at 0.72V) is also demonstrated. The method is applied to the determination of monoamines in individual rat pineals. Low nanogram levels of tyrosine, norepinephrine, 5-hydroxyindole-3-acetic acid, tryptophan, serotonin and 6-hydroxymelatonin, and picogram levels of 5-hydroxytryptophan, 5-hydroxytryptophol, 5-methoxyindole-3-acetic acid, indole 3-acetic acid, 5-methoxytryptophol and melatonin were indicated in most of the samples.

Animals↗
PubMed1991

Melatonin supplementation from early morning auto-urine drinking.

Drinking one's morning urine ('amaroli') is a traditional practice of the yogic religion still widely performed. The pineal hormone melatonin and its conjugated esters are present in morning urine in significant quantities. Drinking the first morning urine restores plasma night-time melatonin levels due to deconjugation of its esters to melatonin. Exogenous melatonin, by either regulation of the sleep-wake cycle or enhancement of the physiological prerequisites for mediation (decreased body awareness (i.e. analgesia) and claimed slowed brain wave activity and heightened visualization ability) may be the mechanism behind the alleged benefits of 'amaroli'.

Humans↗